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Volume 59, Issue 3

sp. nov. and sp. nov., xylanase-producing bacteria isolated from soil Free

Abstract

Two strains of xylanase-producing bacteria, S3-4A and MX2-3, isolated from soils in Thailand, were characterized on the basis of their phenotypic and chemotaxonomic characteristics, DNA–DNA relatedness and 16S rRNA gene sequences. The novel strains were Gram-positive, facultatively anaerobic, spore-forming, rod-shaped bacteria. They contained -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C contents of strains S3-4A and MX2-3 were 52.7 and 52.9 mol%, respectively. The major isoprenoid quinone was MK-7. The dominant cellular fatty acids were anteiso-C and iso-C. Phylogenetic analyses using 16S rRNA gene sequences showed that both novel strains were affiliated to the genus . Strains S3-4A and MX2-3 were closely related to DSM 1355 with 97 % and 97.3 % gene sequence similarities, respectively. The DNA–DNA relatedness between strains S3-4A, MX2-3 and DSM 1355 was low (6.0–30.3 %). The novel strains could be clearly distinguished from DSM 1355 by physiological and biochemical characteristics. Therefore, these two strains represent novel species of the genus , for which the names sp. nov. (type strain S3-4A=KCTC 13043=PCU 275=TISTR 1827) and sp. nov. (type strain MX2-3=KCTC 13044=PCU 276=TISTR 1828) are proposed.

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Keyword(s): NJ, neighbour-joining
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2009-03-01
2023-12-01
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Paenibacillus thailandensis sp. nov. and Paenibacillus nanensis sp. nov., xylanase-producing bacteria isolated from soil
Int J Syst Evol Microbiol 59, 564 (2009); https://doi.org/10.1099/ijs.0.000406-0
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vol. , part 3, pp. 564 - 568

Xylanase activity around colonies of strains S3-4A and MX2-3 on XC medium flooded with Congo red.

Scanning electron micrographs of endospores of strains S3-4A and MX2-3 from 2 day cultures.

Neighbour-joining tree of 16S rRNA gene sequences showing the phylogenetic relationships between strains S3-4A , MX2-3 and all recognized species of the genus .

Maximum-parsimony tree of 16S rRNA gene sequences showing the phylogenetic relationships between strains S3-4A , MX2-3 and all recognized species of the genus .

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