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Abstract

A novel actinomycete, designated strain NEAU-Jh3-20, was isolated from river sediment collected from South river in Jilin Province, north China and characterized using a polyphasic approach. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain NEAU-Jh3-20 should be assigned to the genus and forms a distinct branch with its closest neighbour DSM 41686(97.09 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain NEAU-Jh3-20 to the genus . The cell wall contained -diaminopimelic acid and the whole-cell hydrolysates were glucose and ribose. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were C, C, anteiso-C and anteiso-C. The DNA G+C content was 72.2 mol%. A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-Jh3-20 could be distinguished from its closest phylogenetic relative. Therefore, it is proposed that strain NEAU-Jh3-20 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-Jh3-20 ( = CGMCC 4.7201 = DSM 42170).

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2015-09-01
2021-04-16
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