1887

Abstract

Three neutrotolerant, acidophilic actinomycete strains, designated FXJ1.526, FXJ1.725 and FXJ1.726, were isolated from red soil collected from Liujiazhan, Jiangxi Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the three strains clustered together and their closest relative was CGMCC 4.1985 (98.9–99.0 % similarity). Multilocus sequence analysis confirmed their relationship to and separated these strains as representing a novel species. Mean DNA–DNA hybridization values among strains FXJ1.526, FXJ1.725 and FXJ1.726 were 81.6 ± 3.5–87.2 ± 3.8 %, and the values between the three strains and CGMCC 4.1985 were well below 70 %. The three strains also shared several phenotypic characteristics that were distinct from the closely related species. They grew at 21–50 °C, at pH 4.0–9.0 (with an optimal pH of 5.0) and with 0–3 % (w/v) NaCl, and the major cellular fatty acids were anteiso-C, iso-C and iso-C. On the basis of data from this polyphasic taxonomic study, it is proposed that strains FXJ1.526, FXJ1.725 and FXJ1.726 be classified as representatives of a novel species of the genus , with the name sp. nov. The type strain is FXJ1.725 ( = CGMCC 4.7025 = DSM 42083).

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2015-09-01
2019-12-07
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