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Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK2-2, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK2-2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain SSK2-2 fell within the clade comprising the type strains of species of the genus , joining the type strain of with which it shared 99.4 % similarity. Sequence similarities to the type strains of other species were 94.6–98.2 %. Strain SSK2-2 contained MK-6 as the predominant menaquinone and iso-C 3-OH, iso-C, iso-C G, summed feature 3 (Cω7 and/or Cω6) and summed feature 9 (iso-Cω9 and/or 10-methyl C) as the major fatty acids. The major polar lipids of strain SSK2-2 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-2 was 38.2 mol% and mean levels of DNA–DNA relatedness with the type strains of four phylogenetically related species of the genus were 11–24 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK2-2 is separate from other species. On the basis of the data presented, strain SSK2-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSK2-2 ( = KCTC 42604 = CECT 8869).

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2015-09-01
2019-12-05
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