1887

Abstract

A Gram-stain-positive, endospore-forming, rod-shaped bacterium, designated 1ZS3-5, was isolated from rice rhizosphere in Hunan Province, PR China. The isolate was identified as a member of the genus on the basis of phenotypic characteristics and phylogenetic inference analysis. The 16S rRNA and gene (β-subunit of bacterial RNA polymerase) sequences were closely related to those of CGMCC 1.10966 with similarities of 97.2 % and 89.7 %, respectively. The DNA–DNA hybridization value between 1ZS3-5 and CGMCC 1.10966 was 33.4 %. The DNA G+C content of 1ZS3-5 was 47.5 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid and unknown phospholipid. The predominant respiratory quinone was MK-7. The diamino acid found in the cell-wall peptidoglycan was -diaminopimelic acid. The major cellular fatty acids were anteiso-C, iso-C and iso-C. Based on these results, 1ZS3-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1ZS3-5 ( = ACCC 19782 = DSM 29322).

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2015-09-01
2024-03-29
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