1887

Abstract

A novel, aerobic, psychrotolerant, Gram-stain-positive, endospore-forming strain, NHI-2, was isolated from oil-contaminated soil near a gas station in Mongolia. This strain was characterized by motile rods and grew over a wide range of temperatures ( − 2 to 40 °C) with optimal growth at 28–30 °C. It tolerated salt concentrations of up to 7 % over a five-day incubation period. Analysis of 16S rRNA gene sequence indicated that strain NHI-2 belongs to the genus . Sequence similarity between NHI-2 and members of the genus with validly published names ranged from 97.83 to 98.18 %. DNA–DNA hybridization indicated less than 70 % relatedness to reference strains within the genus. The G+C content of the genomic DNA was 36 mol%. This strain contained MK-8 as a predominant isoprenoid menaquinone. NHI-2 had ornithine in the cell wall similar to reference strains of the genus . The major fatty acids present in NHI-2 were anteiso-C (51.0 %), iso-C (9.1 %) and anteiso-C (8.0 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. These data highlight that the phenotype of strain NHI-2 differs from that of related species in terms of chemotaxonomic properties and genotype characteristics. Therefore, this strain is proposed as a representative of a novel species, named . The type strain is NHI-2 ( = KEMB 9005-135 = KACC 18243 = NBRC 110600).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000375
2015-09-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/3046.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000375&mimeType=html&fmt=ahah

References

  1. Abd El-Rahman H.A. , Fritze D. , Spröer C. , Claus D. . ( 2002;). Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. Int J Syst Evol Microbiol 52: 2127–2133 [CrossRef] [PubMed].
    [Google Scholar]
  2. Barrow G. I. , Feltham R. K. A. . , ( 1993;). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press; [CrossRef].
    [Google Scholar]
  3. Breznak J.A. , Costilow R.N. . ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P. , Murray R. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  4. Darsa K.V. , Thatheyus A.J. , Ramya D. . ( 2014;). Biodegradation of petroleum compound using the bacterium Bacillus subtilis . Science International 2: 20–25.[CrossRef]
    [Google Scholar]
  5. Felsenstein J. . ( 1985;). Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  6. Gudiña E.J. , Pereira J.F.B. , Rodrigues L.R. , Coutinho J.A.P. , Texeira J.A. . ( 2012;). Isolation and study of microorganisms from oil samples for application in Microbial Enhanced Oil Recovery. Int Biodeterior Biodegradation 68: 56–64 [CrossRef].
    [Google Scholar]
  7. Gudiña E.J. , Pereira J.F.B. , Costa R. , Coutinho J.A.P. , Teixeira J.A. , Rodrigues L.R. . ( 2013;). Biosurfactant-producing and oil-degrading Bacillus subtilis strains enhance oil recovery in laboratory sand-pack columns. J Hazard Mater 261: 106–113 [CrossRef] [PubMed].
    [Google Scholar]
  8. Hairaishi A. , Ueda Y. , Ishihara J. , Mori T. . ( 1996;). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42: 457–469 [CrossRef].
    [Google Scholar]
  9. Kempf M.J. , Chen F. , Kern R. , Venkateswaran K. . ( 2005;). Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility. Astrobiology 5: 391–405 [CrossRef] [PubMed].
    [Google Scholar]
  10. Khan J.A. , Asthana A. . ( 2011;). A study on oil degradation potential of Bacillus megaterium isolated from oil contaminated sites in Lucknow. Arch Appl Sci Res 3: 513–517.
    [Google Scholar]
  11. Kim O.S. , Cho Y.J. , Lee K. , Yoon S.H. , Kim M. , Na H. , Park S.C. , Jeon Y.S. , Lee J.H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  12. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  13. Krishnamurthi S. , Ruckmani R. , Pukall R. , Chakrabarti T. . ( 2010;). Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov.. Syst Appl Microbiol 33: 367–373 [CrossRef].
    [Google Scholar]
  14. Larkin J.M. , Stokes J.L. . ( 1967;). Taxonomy of psychrophilic strains of Bacillus . J Bacteriol 94: 889–895 [PubMed].
    [Google Scholar]
  15. Mehlen A. , Goeldner M. , Ried S. , Stindl S. , Ludwig W. , Schleifer K.H. . ( 2004;). Development of a fast DNA-DNA hybridization method based on melting profiles in microplates. Syst Appl Microbiol 27: 689–695 [CrossRef] [PubMed].
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W.B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  17. Minnikin D.E. , O'Donnell A.G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J.H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  18. Moore E.R.B. , Arnscheidt A. , Krüger A. , Strömpl C. , Mau M. . ( 2004;). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Molecular Microbial Ecology Manual , 2nd edn.. pp. 3–18. Edited by Kowalchuk G. A. , de Bruijn F. J. , Head I. M. , Akkermans A. D. L. , van Elsas J. D. . Dordrecht: Kluwer;.
    [Google Scholar]
  19. Murray R.G.E. , Doetsch R.N. , Robinow C.F. . ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  20. Nei M. , Kumar S. . ( 2000;). Molecular Evolution and Phylogenetics New York: Oxford University Press;.
    [Google Scholar]
  21. Nwaogu L.A. , Onyeze G.O.C. , Nwabueze R.N. . ( 2008;). Degradation of diesel oil in a polluted soil using Bacillus subtilis . African Journal of Biotechnology 7: 1939–1943.
    [Google Scholar]
  22. Ouoba L.I.I. , Cantor M.D. , Diawara B. , Traoré A.S. , Jakobsen M. . ( 2003;). Degradation of African locust bean oil by Bacillus subtilis and Bacillus pumilus isolated from soumbala, a fermented African locust bean condiment. J Appl Microbiol 95: 868–873 [CrossRef] [PubMed].
    [Google Scholar]
  23. Pereira J.F.B. , Gudiña E.J. , Costa R. , Vitorino R. , Teixeira J.A. , Coutinho J.A.P. , Rodrigues L.R. . ( 2013;). Optimization and characterization of biosurfactant production by Bacillus subtilis isolated towards microbial enhanced oil recovery applications. Fuel 111: 259–268.[CrossRef]
    [Google Scholar]
  24. Pham H.T.V. , Kim J. . ( 2014;). Bacillus thaonhiensis sp. nov., a new species, was isolated from the forest soil of Kyonggi University by using a modified culture method. Curr Microbiol 68: 88–95 [CrossRef] [PubMed].
    [Google Scholar]
  25. Rautela G.S. , Cowling E.B. . ( 1966;). Simple cultural test for relative cellulolytic activity of fungi. Appl Microbiol 14: 892–898 [PubMed].
    [Google Scholar]
  26. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  27. Schumann P. . ( 2011;). Peptidoglycan structure. Methods Microbiol 38: 101–129.[CrossRef]
    [Google Scholar]
  28. Smibert R.M. , Krieg N.R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  29. Swan A. . ( 1954;). The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci). J Clin Pathol 7: 160–163 [CrossRef] [PubMed].
    [Google Scholar]
  30. Tamaoka J. , Komagata K. . ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25: 125–128 [CrossRef].
    [Google Scholar]
  31. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  32. Vermeulen J. . ( 2007;). Ripening of PAH and TPH polluted sediments: determination and qualification of bioremediation parameters. PhD thesis, Wageningen University, Wageningen, The Netherlands.
  33. Yan S. , Wang Q. , Qu L. , Li C. . ( 2013;). Characterization of oil-degrading bacteria from oil-contaminated soil and activity of their enzymes. Biotechnol Biotechnol Equip 27: 3932–3938 [CrossRef].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000375
Loading
/content/journal/ijsem/10.1099/ijs.0.000375
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error