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Abstract

A novel, aerobic, psychrotolerant, Gram-stain-positive, endospore-forming strain, NHI-2, was isolated from oil-contaminated soil near a gas station in Mongolia. This strain was characterized by motile rods and grew over a wide range of temperatures ( − 2 to 40 °C) with optimal growth at 28–30 °C. It tolerated salt concentrations of up to 7 % over a five-day incubation period. Analysis of 16S rRNA gene sequence indicated that strain NHI-2 belongs to the genus . Sequence similarity between NHI-2 and members of the genus with validly published names ranged from 97.83 to 98.18 %. DNA–DNA hybridization indicated less than 70 % relatedness to reference strains within the genus. The G+C content of the genomic DNA was 36 mol%. This strain contained MK-8 as a predominant isoprenoid menaquinone. NHI-2 had ornithine in the cell wall similar to reference strains of the genus . The major fatty acids present in NHI-2 were anteiso-C (51.0 %), iso-C (9.1 %) and anteiso-C (8.0 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. These data highlight that the phenotype of strain NHI-2 differs from that of related species in terms of chemotaxonomic properties and genotype characteristics. Therefore, this strain is proposed as a representative of a novel species, named . The type strain is NHI-2 ( = KEMB 9005-135 = KACC 18243 = NBRC 110600).

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2015-09-01
2020-08-12
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