A Gram-stain positive, strictly aerobic, non-motile and coccus-shaped actinobacterium, designated strain NEAU-ST5-33T, was isolated from saline and alkaline soils in Dechang Township, Zhaodong City, PR China. It formed beige-yellow colonies and grew at NaCl concentrations of 0–5 % (w/v) (optimum 0 %), at pH 6.0–9.0 (optimum pH 7.0) and over a temperature range of 4–50 °C (optimum 35 °C). Based on 16S rRNA gene sequence analysis, strain NEAU-ST5-33T was phylogenetically closely related to the type strains of species of the genus Kocuria, Kocuria polaris CMS 76orT, Kocuria rosea DSM 20447T, Kocuria turfanensis HO-9042T, Kocuria aegyptia YIM 70003T, Kocuria himachalensis K07-05T and Kocuria flava HO-9041T, with respective sequence similarities of 98.8 %, 98.8 %, 98.3 %, 98.1 %, 98.1 % and 97.9 %. DNA–DNA hybridization relatedness values of strain NEAU-ST5-33T with type strains of the closely related species ranged from 54 ± 1 % to 34 ± 1 %. The DNA G+C content was 61.2 mol%. The major fatty acids (>5 %) were C15 : 0 anteiso, C15 : 0 iso and C16 : 1ω7c and/or C16 : 1ω6c. The major menaquinone detected was MK-8 (H2), and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unknown aminolipid and one unknown lipid. On the basis of the genotypic, chemotaxonomic and phenotypic data, we propose that strain NEAU-ST5-33T represents a novel species of the genus Kocuria, with the name Kocuria dechangensis sp. nov. The type strain is NEAU-ST5-33T ( = CGMCC 1.12187T = DSM 25872T).
CernyG.
( 1978;). Studies on aminopeptidase test for the distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol5113–122 [View Article].
De LeyJ.,
CattoirH.,
ReynaertsA.
( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12133–142 [View Article][PubMed].
HussV.A.R.,
FestlH.,
SchleiferK.H.
( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4184–192 [View Article][PubMed].
KimuraM.
( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16111–120 [View Article][PubMed].
MarmurJ.,
DotyP.
( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5109–118 [View Article][PubMed].
MayilrajS.,
KroppenstedtR.M.,
SureshK.,
SainiH.S.
( 2006;). Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol561971–1975 [View Article][PubMed].
RaineyF.A.,
NobreM.F.,
SchumannP.,
StackebrandtE.,
da CostaM.S.
( 1997;). Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol47510–514 [View Article][PubMed].
SehgalS.N.,
GibbonsN.E.
( 1960;). Effect of some metal ions on the growth of Halobacterium cutirubrum
. Can J Microbiol6165–169 [View Article][PubMed].
SmibertR.M.,
KriegN.R.
( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC:
American Society for Microbiology;.
StackebrandtE.,
KochC.,
GvozdiakO.,
SchumannP.
( 1995;). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol45682–692 [View Article][PubMed].
WayneL.G.,
BrennerD.J.,
ColwellR.R.,
GrimontP.A.D.,
KandlerO.,
KrichevskyM.I.,
MooreL.H.,
MooreW.E.C.,
MurrayR.G.E.,
other authors.
( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37463–464 [View Article].