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Abstract

An extremely halophilic archaeon was isolated from a water sample of Isla Bacuta saltern in Huelva, Spain. Strain ASP54 is a novel red-pigmented, motile, rod-shaped, Gram-stain-negative and strictly aerobic haloarchaeon. Strain ASP54 grew in media containing 15–30  % (w/v) salts and optimally with 25  % (w/v) salts. It grew between pH 5.0 and 9.0 (optimally at pH 7.5) and at 20–40 °C (optimally at 37 °C). Phylogenetic analysis based on multi-locus sequence analysis (MLSA) and the comparison of 16S rRNA gene sequences revealed that strain ASP54 is most closely related to the genus . The closest relatives were EB27 (92.1  % 16S rRNA gene sequence similarity), TNN28 (92.1  %), and D108 (92.0  %). The polar lipid pattern of strain ASP54 consisted of biphosphatidylglycerol, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether and a minor-phospholipid. The predominant respiratory quinone was menaquinone-8 (MK-8) (83  %), and a minor amount of MK-8(VIII-H) (17  %) was also detected. The G+C content of the genomic DNA of this strain was 63.1 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain ASP54 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ASP54 ( = CECT 8749 = IBRC-M 10946 = JCM 30072).

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2015-09-01
2020-08-10
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