1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_9

sp. nov., an extremely halophilic archaeon isolated from a saltern Free

Abstract

An extremely halophilic archaeon was isolated from a water sample of Isla Bacuta saltern in Huelva, Spain. Strain ASP54 is a novel red-pigmented, motile, rod-shaped, Gram-stain-negative and strictly aerobic haloarchaeon. Strain ASP54 grew in media containing 15–30  % (w/v) salts and optimally with 25  % (w/v) salts. It grew between pH 5.0 and 9.0 (optimally at pH 7.5) and at 20–40 °C (optimally at 37 °C). Phylogenetic analysis based on multi-locus sequence analysis (MLSA) and the comparison of 16S rRNA gene sequences revealed that strain ASP54 is most closely related to the genus . The closest relatives were EB27 (92.1  % 16S rRNA gene sequence similarity), TNN28 (92.1  %), and D108 (92.0  %). The polar lipid pattern of strain ASP54 consisted of biphosphatidylglycerol, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether and a minor-phospholipid. The predominant respiratory quinone was menaquinone-8 (MK-8) (83  %), and a minor amount of MK-8(VIII-H) (17  %) was also detected. The G+C content of the genomic DNA of this strain was 63.1 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain ASP54 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ASP54 ( = CECT 8749 = IBRC-M 10946 = JCM 30072).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000370
2015-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/3016.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000370&mimeType=html&fmt=ahah

References

  1. Amoozegar M.A., Makhdoumi-Kakhki A., Mehrshad M., Fazeli S.A., Spröer C., Ventosa A. ( 2014;). Halorientalis persicus sp. nov., an extremely halophilic archaeon isolated from a salt lake and emended description of the genus Halorientalis. Int J Syst Evol Microbiol 64 940–944. [CrossRef]
    [Google Scholar]
  2. Angelini R., Corral P., Lopalco P., Ventosa A., Corcelli A. ( 2012;). Novel ether lipid cardiolipins in archaeal membranes of extreme haloalkaliphiles. Biochim Biophys Acta 1818 1365–1373 [View Article] [PubMed] .
     [Google Scholar]
  3. Barrow G. I., Feltham R. K. A. ), ( 2003). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn.., Cambridge: Cambridge University Press;.
     [Google Scholar]
  4. Bauer A.W., Kirby W.M.M., Sherris J.C., Turck M. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45 493–496 [PubMed].
     [Google Scholar]
  5. Boucher Y., Douady C.J., Sharma A.K., Kamekura M., Doolittle W.F. ( 2004;). Intragenomic heterogeneity and intergenomic recombination among haloarchaeal rRNA genes. J Bacteriol 186 3980–3990 [View Article] [PubMed] .
     [Google Scholar]
  6. Clarke P.H. ( 1953;). Hydrogen sulphide production by bacteria. J Gen Microbiol 8 397–407 [View Article] [PubMed] .
     [Google Scholar]
  7. Corcelli A., Lobasso S. ( 2006;). Characterization of lipids of halophilic archaea. . In Methods in Microbiology, Extremophiles, pp. 585–613. Edited by Rainey F. A., Oren A. Amsterdam: Elsevier/Academic;.
     [Google Scholar]
  8. Corral P., Gutiérrez M.C., Castillo A.M., Domínguez M., Lopalco P., Corcelli A., Ventosa A. ( 2013;). Natronococcus roseus sp. nov., a haloalkaliphilic archaeon from a hypersaline lake. Int J Syst Evol Microbiol 63 104–108 [View Article] [PubMed] .
     [Google Scholar]
  9. Cui H.L., Yang X., Gao X., Xu X.W. ( 2011;). Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae. Int J Syst Evol Microbiol 61 2682–2689. [CrossRef]
    [Google Scholar]
  10. DeLong E.F. ( 1992;). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89 5685–5689 [View Article] [PubMed] .
     [Google Scholar]
  11. Dyall-Smith M. ( 2009). The halohandbook: protocols for haloarchaeal genetics v.7.2, pp. 118 http://www.haloarchaea.com/resources/halohandbook/.
     [Google Scholar]
  12. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  13. Fernández A.B., Ghai R., Martin-Cuadrado A.B., Sánchez-Porro C., Rodriguez-Valera F., Ventosa A. ( 2014a;). Prokaryotic taxonomic and metabolic diversity of an intermediate salinity hypersaline habitat assessed by metagenomics. FEMS Microbiol Ecol 88 623–635 [View Article] [PubMed] .
     [Google Scholar]
  14. Fernández A.B., Vera-Gargallo B., Sánchez-Porro C., Ghai R., Papke R.T., Rodriguez-Valera F., Ventosa A. ( 2014b;). Comparison of prokaryotic community structure from Mediterranean and Atlantic saltern concentrator ponds by a metagenomic approach. Front Microbiol 5 196 [View Article] [PubMed] .
     [Google Scholar]
  15. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  16. Fullmer M.S., Soucy S.M., Swithers K.S., Makkay A.M., Wheeler R., Ventosa A., Gogarten J.P., Papke R.T. ( 2014;). Population and genomic analysis of the genus Halorubrum . Front Microbiol 5 140 [View Article] [PubMed] .
     [Google Scholar]
  17. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. ), ( 1994). Methods for General and Molecular Bacteriology., Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  18. Ghai R., Pašić L., Fernández A.B., Martin-Cuadrado A.B., Mizuno C.M., McMahon K.D., Papke R.T., Stepanauskas R., Rodriguez-Brito B., other authors. ( 2011;). New abundant microbial groups in aquatic hypersaline environments. Sci Rep 1 135 [View Article] [PubMed] .
     [Google Scholar]
  19. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 95–98.
     [Google Scholar]
  20. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed] .
     [Google Scholar]
  21. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32 1363–1371 [View Article] [PubMed] .
     [Google Scholar]
  22. Makhdoumi-Kakhki A., Amoozegar M.A., Ventosa A. ( 2012;). Halovenus aranensis gen. nov., sp. nov., an extremely halophilic archaeon from Aran-Bidgol salt lake. Int J Syst Evol Microbiol 62 1331–1336 [View Article] [PubMed] .
     [Google Scholar]
  23. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208–218 [View Article].
     [Google Scholar]
  24. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109–118 [View Article] [PubMed] .
     [Google Scholar]
  25. Nelson-Sathi S., Dagan T., Landan G., Janssen A., Steel M., McInerney J.O., Deppenmeier U., Martin W.F. ( 2012;). Acquisition of 1,000 eubacterial genes physiologically transformed a methanogen at the origin of Haloarchaea. Proc Natl Acad Sci U S A 109 20537–20542 [View Article] [PubMed] .
     [Google Scholar]
  26. Oren A., Ventosa A. ( 2013;). Subcommittee on the taxonomy of Halobacteriaceae and Subcommittee on the taxonomy of Halomonadaceae: minutes of the joint open meeting, 24 June 2013, Storrs, Connecticut, USA. Int J Syst Evol Microbiol 63 3540–3544 [View Article] [PubMed] .
     [Google Scholar]
  27. Oren A., Ventosa A., Grant W.D. ( 1997;). Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47 233–238 [View Article].
     [Google Scholar]
  28. Owen R.J., Hill L.R. ( 1979;). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. . In Identification Methods for Microbiologists (Society for Applied Bacteriology Technical Series no. 14), pp. 277–296. Edited by Skinner F. A., Lovelock D. W. , 2nd edn.., London: Academic Press;.
     [Google Scholar]
  29. Papke R.T., White E., Reddy P., Weigel G., Kamekura M., Minegishi H., Usami R., Ventosa A. ( 2011;). A multilocus sequence analysis approach to the phylogeny and taxonomy of the Halobacteriales . Int J Syst Evol Microbiol 61 2984–2995 [View Article] [PubMed] .
     [Google Scholar]
  30. Ram Mohan N., Fullmer M.S., Makkay A.M., Wheeler R., Ventosa A., Naor A., Gogarten J.P., Papke R.T. ( 2014;). Evidence from phylogenetic and genome fingerprinting analyses suggests rapidly changing variation in Halorubrum and Haloarcula populations. Front Microbiol 5 143 [View Article] [PubMed] .
     [Google Scholar]
  31. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  32. Sambrook J., Russell D.W. ( 2001). Molecular Cloning: a Laboratory Manual , 3rd edn.., Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;.
     [Google Scholar]
  33. Scorpio R. ( 2000;). Fundamentals of Acids. . In Bases, Buffers and Their Application to Biochemical Systems., Iowa: Kendall Hunt Publishing Company;.
     [Google Scholar]
  34. Subov N.N. ( 1931). Oceanographical Tables., Moscow: USSR Oceanographic Institute Hydrometeorological Commission;.
     [Google Scholar]
  35. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed] .
     [Google Scholar]
  36. Ventosa A., Quesada E., Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982;). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128 1959–1968.
     [Google Scholar]
  37. Williams D., Gogarten J.P., Papke R.T. ( 2012;). Quantifying homologous replacement of loci between haloarchaeal species. Genome Biol Evol 4 1223–1244 [View Article] [PubMed] .
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000370
Loading
Halovenus salina sp. nov., an extremely halophilic archaeon isolated from a saltern
Int J Syst Evol Microbiol 65, 3016 (2015); https://doi.org/10.1099/ijs.0.000370
/content/journal/ijsem/10.1099/ijs.0.000370
/content/journal/ijsem/10.1099/ijs.0.000370
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error