1887

Abstract

A Gram-staining-negative, non-motile, catalase- and oxidase-positive, facultatively aerobic bacterium, designated IMCC1962 was isolated from a surface seawater sample from the Yellow Sea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC1962 belonged to the genus , forming a robust clade with members of the genus, and was most closely related to (97.3  % similarity). DNA–DNA relatedness between strain IMCC1962 and DSM 19752 was 21.8–26.3  %, which indicated strain IMCC1962 represents a novel genomic species of the genus . The G+C content of the DNA of strain IMCC1962 was 48.7 mol%. The major isoprenoid quinone was ubiquinone Q-8 and major fatty acids were Cω7 and/or Cω6 (43.4  %), Cω7 and/or Cω6 (19.3  %) and C (17.2  %). The polar lipids found in strain IMCC1962 were phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid, unknown phospholipid, and four unknown polar lipids. Strain IMCC1962 and DSM 19752 differed from each other in diverse phenotypic characteristics including motility, colony colour and enzyme activities. On the basis of phenotypic and genotypic data, strain IMCC1962 ( = KACC 17481 = NBRC 109703) represents a novel species of the genus for which the name sp. nov. is proposed. An emended description of the genus is also provided.

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2015-09-01
2019-10-16
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References

  1. Bernardet J.-F. , Nakagawa Y. , Holmes B. . & Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [CrossRef] [PubMed].
    [Google Scholar]
  2. Cho J.-C. , Giovannoni S.J. . ( 2003;). Parvularcula bermudensis gen. nov. sp. nov., a marine bacterium that forms a deep branch in the. α-Proteobacteria. Int J Syst Evol Microbiol 53: 1031–1036 [CrossRef] [PubMed].
    [Google Scholar]
  3. Collins M.D. . ( 1985;). Analysis of isoprenoid quinones. Methods Microbiol 18: 329–363.[CrossRef]
    [Google Scholar]
  4. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  5. Fitch W.M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 27: 401–410.
    [Google Scholar]
  6. Haygood M.G. , Davidson S.K. , Small-subunit . ( 1997;). rRNA genes and in situ hybridization with oligonucleotides specific for the bacterial symbionts in the larvae of the bryozoan Bugula neritina and proposal of “Candidatus endobugula sertula. ”. Appl Environ Microbiol 63: 4612–4616 [PubMed].
    [Google Scholar]
  7. Jukes T.H. , Cantor C.R. . ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–32. Edited by Munro H. N. . New York: [CrossRef] Academic Press;.
    [Google Scholar]
  8. Kim O.S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y.S. , Lee J.-H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  9. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19: 161–206.[CrossRef]
    [Google Scholar]
  10. Lim G.E. , Haygood M.G. . ( 2004;). Candidatus Endobugula glebosa,” a specific bacterial symbiont of the marine bryozoan Bugula simplex . Appl Environ Microbiol 70: 4921–4929 [CrossRef] [PubMed].
    [Google Scholar]
  11. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar Buchner A. , Lai T. , Steppi S. , other authors . ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32: 1363–1371 [CrossRef] [PubMed].
    [Google Scholar]
  12. Lyman J. , Fleming R.H. . ( 1940;). Composition of sea water. J Mar Res 3: 134–146.
    [Google Scholar]
  13. Mesbah M. , Premachandran U. , Whitman W.B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  14. Minnikin D.E. , O'Donnell A.G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J.H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  15. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  16. Stackebrandt E. , Goebel B.M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  17. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). “mega”6: molecular evolutionary genetics analysis version 6.0.. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  18. Tindall B.J. , Sikorski J. , Smibert R.M. , Krieg N.R. . ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology , 3rd edn.., pp. 330–393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. A. . Washington, DC: [CrossRef] American Society for Microbiology;.
    [Google Scholar]
  19. Urios L. , Intertaglia L. , Lesongeur F. , Lebaron P. . ( 2011;). Eionea nigra gen. nov. sp. nov., a gammaproteobacterium from the Mediterranean Sea. Int J Syst Evol Microbiol 61: 1677–1681 [CrossRef] [PubMed].
    [Google Scholar]
  20. Wayne L.G. , Brenner D.J. , Colwell R.R. , Grimont P.A. , Kandler O. , Krichevsky M.I. , Moore L.H. , Moore W.E.C. , Murray R.G.E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
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