1887

Abstract

Four strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16S rRNA and 23S rRNA gene sequence analyses and DNA–DNA reassociation data revealed that the strains constituted a novel group within the phylum ‘’ and were most closely related to . Two libraries of randomly cloned DNA were prepared from strain W5455 and were sequenced to provide a genome survey as a resource for metagenomic studies. A new genus and novel species, gen. nov., sp. nov., is proposed to accommodate these strains. The genus comprises strains that are anaerobic, non-motile, asaccharolytic bacilli that produce acetic and isovaleric acids and minor to trace amounts of propionic, isobutyric, succinic and phenylacetic acids as end products of metabolism. gen. nov., sp. nov. produced hydrogen sulphide but was otherwise largely biochemically unreactive. Growth was stimulated by the addition of glycine to broth media. The G+C content of the DNA of the type strain was 59 mol%. The type strain of sp. nov. is W5455 (=DSM 21147=CCUG 55836).

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2009-05-01
2019-10-16
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16S-23S rRNA gene intervening sequences and 23S rRNA primers. [PDF](42 KB)

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Fatty acid methyl ester compositions of strains W5455 and W5706 and profiles for related members of the phylum ' '. [PDF](14 KB)

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Static annotation of partial and full-length open-reading frames in genomic library 1. [excel file]

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Static annotation of partial and full-length open-reading frames in genomic library 2. [excel file]

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