1887

Abstract

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterium, designated CAU 1183, was isolated from marine sand and its taxonomic position was investigated by using a polyphasic approach. The bacterium grew optimally at 30 °C, at pH 8.5 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1183 formed a distinct lineage within the genus and exhibited the highest similarity to CAU 1051 (97.6 %). The strain contained MK-7 as the predominant isoprenoid quinone and anteiso-C :  was the major cellular fatty acid. The cell-wall peptidoglycan contained -diaminopimelic acid. The polar lipid pattern of strain CAU 1183 consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified lipids, including two phospholipids, two glycolipids, a phosphoglycolipid and two lipids. The G+C content of the genomic DNA was 37.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CAU 1183 should be assigned to a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CAU 1183 ( = KCTC 33037 = CECT 8560).

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2015-09-01
2024-12-14
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References

  1. Amoozegar M.A., Bagheri M., Makhdoumi-Kakhki A., Didari M., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. ( 2014;). Oceanobacillus limi sp. nov., a moderately halophilic bacterium from a salt lake. Int J Syst Evol Microbiol 64 12841289 [View Article] [PubMed].
    [Google Scholar]
  2. Bowman J.P. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50 18611868 [PubMed]. [CrossRef]
    [Google Scholar]
  3. Cappuccino J.G., Sherman N. ( 2002). Microbiology: a Laboratory Manual , 6th edn.., Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  4. Cho S.-L., Nam S.-W., Yoon J.-H., Lee J.-S., Sukhoom A., Kim W. ( 2008;). Lactococcus chungangensis sp. nov., a lactic acid bacterium isolated from activated sludge foam. Int J Syst Evol Microbiol 58 18441849 [PubMed]. [CrossRef]
    [Google Scholar]
  5. Conn H.J., Bartholomew J.W., Jennison M.W. ( 1957). Staining methods. In Manual of Microbiological Methods, pp. 30–36. Edited by the Society of American Bacteriologists; New York: McGraw-Hill:.
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  7. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  8. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  9. Felsenstein J. ( 1989;). phylip – phylogeny inference package (version 3.2). Cladistics 5 164166.
    [Google Scholar]
  10. Fitch W.M., Margoliash E. ( 1967;). Construction of phylogenetic trees. Science 155 279284 [View Article] [PubMed].
    [Google Scholar]
  11. Gordon R.E., Mihm J.M. ( 1962;). Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98 628636. [CrossRef]
    [Google Scholar]
  12. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. ( 1998;). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44 11481153 [View Article].
    [Google Scholar]
  13. Heyrman J., Logan N.A., Busse H.-J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. ( 2003;). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53 501511 [View Article] [PubMed].
    [Google Scholar]
  14. Hiraishi A., Hoshino Y., Kitamura H. ( 1984;). Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 30 197210 [View Article].
    [Google Scholar]
  15. Hirota K., Aino K., Nodasaka Y., Yumoto I. ( 2013a;). Oceanobacillus indicireducens sp. nov., a facultative alkaliphile that reduces an indigo dye. Int J Syst Evol Microbiol 63 14371442 [View Article] [PubMed].
    [Google Scholar]
  16. Hirota K., Hanaoka Y., Nodasaka Y., Yumoto I. ( 2013b;). Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid. Int J Syst Evol Microbiol 63 33073312 [View Article] [PubMed].
    [Google Scholar]
  17. Jukes T.H., Cantor C.R. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. H. 3 New York: Academic Press;. [CrossRef]
    [Google Scholar]
  18. Kim Y.-G., Choi D.H., Hyun S., Cho B.C. ( 2007;). Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol 57 409413 [View Article] [PubMed].
    [Google Scholar]
  19. Lányi B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 167. [CrossRef]
    [Google Scholar]
  20. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., other authors. ( 2007;). clustal w clustal_x version 2.0. Bioinformatics 23 29472948 [View Article] [PubMed].
    [Google Scholar]
  21. Lee S.-Y., Oh T.-K., Kim W., Yoon J.H. ( 2010;). Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 60 27582762 [View Article] [PubMed].
    [Google Scholar]
  22. Lee D.C., Kang H., Weerawongwiwat V., Kim B., Choi Y.-W., Kim W. ( 2013;). Oceanobacillus chungangensis sp. nov., isolated from a sand dune. Int J Syst Evol Microbiol 63 36663671 [View Article] [PubMed].
    [Google Scholar]
  23. Lu J., Nogi Y., Takami H. ( 2001;). Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205 291297 [View Article] [PubMed].
    [Google Scholar]
  24. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  25. Minnikin D.E., Hutchinson I.G., Caldicott A.B., Goodfellow M. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188 221233. [CrossRef]
    [Google Scholar]
  26. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  27. Nam J.-H., Bae W., Lee D.-H. ( 2008;). Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. Int J Syst Evol Microbiol 58 11091113 [View Article] [PubMed].
    [Google Scholar]
  28. Namwong S., Tanasupawat S., Lee K.C., Lee J.-S. ( 2009;). Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 59 22542259 [View Article] [PubMed].
    [Google Scholar]
  29. Nicholson W.L., Setlow P. ( 1990;). Sporulation, germination and outgrowth. . In Molecular Biological Methods for Bacillus, pp. 391450. Edited by Harwood C. R., Cutting S. M. Chichester: Wiley;.
    [Google Scholar]
  30. Raats D., Halpern M. ( 2007;). Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int J Syst Evol Microbiol 57 255259 [View Article] [PubMed].
    [Google Scholar]
  31. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  32. Schleifer K.H., Seidl P.H. ( 1985;). Chemical composition and structure of murein. . In Chemical Methods in Bacterial Systematics, pp. 201219. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  33. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  34. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128. [CrossRef]
    [Google Scholar]
  35. Tominaga T., An S.Y., Oyaizu H., Yokota A. ( 2009;). Oceanobacillus soja sp. nov. isolated from soy sauce production equipment in Japan. J Gen Appl Microbiol 55 225232 [View Article] [PubMed].
    [Google Scholar]
  36. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  37. Whon T.W., Jung M.-J., Roh S.W., Nam Y.-D., Park E.-J., Shin K.-S., Bae J.-W. ( 2010;). Oceanobacillus kimchii sp. nov. isolated from a traditional Korean fermented food. J Microbiol 48 862866 [View Article] [PubMed].
    [Google Scholar]
  38. Wu M., Yang G., Yu Z., Zhuang L., Jin Y., Zhou S. ( 2014;). Oceanobacillus luteolus sp. nov., isolated from soil. Int J Syst Evol Microbiol 64 14951500 [View Article] [PubMed].
    [Google Scholar]
  39. Yang J.-Y., Huo Y.-Y., Xu X.-W., Meng F.-X., Wu M., Wang C.-S. ( 2010;). Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor. Int J Syst Evol Microbiol 60 24092414 [View Article] [PubMed].
    [Google Scholar]
  40. Yu C., Yu S., Zhang Z., Li Z., Zhang X.-H. ( 2014;). Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment. Int J Syst Evol Microbiol 64 12781283 [View Article] [PubMed].
    [Google Scholar]
  41. Yumoto I., Hirota K., Nodasaka Y., Nakajima K. ( 2005;). Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus . Int J Syst Evol Microbiol 55 15211524 [View Article] [PubMed].
    [Google Scholar]
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