RT Journal Article SR Electronic(1) A1 Ren, Yong-Cheng A1 Liu, Si-Tong A1 Li, Ying A1 Hui, Feng-LiYR 2015 T1 Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_9 SP 2875 OP 2881 DO https://doi.org/10.1099/ijs.0.000349 PB Microbiology Society, SN 1466-5034, AB Seven yeast strains were isolated from the gut of insect larvae and decayed wood, which were collected from three localities near Nanyang, Henan Province, China. These strains were identified as two novel species through comparison of sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and other taxonomic characteristics. Pichia dushanensis sp. nov. was closely related to species in the Pichia clade and produced one to four spheroid ascospores in a deliquescent ascus. The D1/D2 sequence of P. dushanensis sp. nov. differed from its closest relative, Issatchenkia (Pichia) sp. NRRL Y-12824, by 3.6  % sequence divergence (16 substitutions and 4 gaps). The species also differed from its four closest known species, Candida rugopelliculosa, Pichia occidentalis, Pichia exigua and Candida phayaonensis, by 4.1–4.4  % sequence divergence (22–24 substitutions and 0–2 gaps) in the D1/D2 sequences. Hyphopichia paragotoi sp. nov. belonged to the Hyphopichia clade, and its nearest phylogenetic neighbours were Candida gotoi, Candida pseudorhagii, Candida rhagii and Hyphopichia heimii with 3.2–4.2  % sequence divergence (16–21substitutions and 1 gap) in the D1/D2 sequences. In comparison with previously established species, H. paragotoi sp. nov. formed one hat-shaped ascospore in a persistent ascus. The type strain of P. dushanensis sp. nov. is NYNU 14658T ( = CICC 33049T = CBS 13912T), and the type strain of H. paragotoi sp. nov. is NYNU 14666T ( = CICC 33048T = CBS 13913T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000349