1887

Abstract

A novel thermophilic, obligately anaerobic bacterium, strain Cel2f, was isolated from a cellulolytic community enriched from coastal marine sediment. Cells were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Optimal growth temperature and pH of strain Cel2f were 55 °C and pH 7.0, respectively. NaCl was essential for the growth of strain Cel2f and the strain showed enhanced growth in the presence of sea salt; the optimum sea salt concentration for growth was 7 % (w/v). Thiosulfate, sulfate and sulfite were potential electron acceptors. The major fatty acids of strain Cel2f were iso-C, C, and C. Polar lipid analysis indicated the presence of phosphatidylethanolamine and phosphatidylglycerol. Strain Cel2f contained menaquinone MK-7 as the isoprenoid quinone, and the DNA G+C content was 31.3 mol%. Phylogenetic analysis revealed that the nearest relative of strain Cel2f was BM with 93.8 % 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain Cel2f represents a novel species of genus , for which the name sp. nov. is proposed. The type strain is Cel2f ( = JCM 30480 = CGMCC 1.5200).

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2015-09-01
2019-10-15
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References

  1. Chenna R. , Sugawara H. , Koike T. , Lopez R. , Gibson T.J. , Higgins D.G. , Thompson J.D. . ( 2003;). Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res 31: 3497–3500 [CrossRef] [PubMed].
    [Google Scholar]
  2. Fang M.-X. , Zhang W.-W. , Zhang Y.-Z. , Tan H.-Q. , Zhang X.-Q. , Wu M. , Zhu X.-F. . ( 2012;). Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater. Int J Syst Evol Microbiol 62: 3018–3023 [CrossRef] [PubMed].
    [Google Scholar]
  3. Gauthier M.J. , Lafay B. , Christen R. , Fernandez L. , Acquaviva M. , Bonin P. , Bertrand J.-C. . ( 1992;). Marinobacter hydrocarbonoclasticus gen. nov. sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42: 568–576 [CrossRef] [PubMed].
    [Google Scholar]
  4. Goodfellow M. , Manfio G. , Chun J. . ( 1997;). Towards a practical species concept for cultivable bacteria. . In Species: the Units of Biodiversity, pp. 25–29. Edited by Clarridge M. F. , Dawah H. A. . London: Chapman and Hall;.
    [Google Scholar]
  5. Huber R. , Langworthy T.A. , König H. , Thomm M. , Woese C.R. , Sleytr U.B. , Stetter K.O. . ( 1986;). Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch Microbiol 144: 324–333 [CrossRef].
    [Google Scholar]
  6. Ji S. , Wang S. , Tan Y. , Chen X. , Schwarz W. , Li F. . ( 2012;). An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions. FEMS Microbiol Lett 335: 39–46 [CrossRef] [PubMed].
    [Google Scholar]
  7. Kawamoto I. , Oka T. , Nara T. . ( 1981;). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146: 527–534 [PubMed].
    [Google Scholar]
  8. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y.S. , Lee J.-H. . otherauthors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  9. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  10. Kuhnert P. , Frey J. , Lang N.P. , Mayfield L. . ( 2002;). Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia, Porphyromonas gingivalis clinical strains reveals a clear species clustering. Int J Syst Evol Microbiol 52: 1391–1395 [CrossRef] [PubMed].
    [Google Scholar]
  11. Mesbah M. , Premachandran U. , Whitman W.B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  12. Minnikin D.E. , O'Donnell A.G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J.H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  13. Moore L.V. , Bourne D.M. , Moore W.E. . ( 1994;). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacteriol 44: 338–347 [CrossRef] [PubMed].
    [Google Scholar]
  14. Ogg C.D. , Patel B.K. . ( 2009;). Thermotalea metallivorans gen. nov., sp. nov., a thermophilic, anaerobic bacterium from the Great Artesian Basin of Australia aquifer. Int J Syst Evol Microbiol 59: 964–971 [CrossRef] [PubMed].
    [Google Scholar]
  15. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  16. Smibert R.M. , Krieg N.R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . .
    [Google Scholar]
  17. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  18. West M. , Burdash N.M. , Freimuth F. . ( 1977;). Simplified silver-plating stain for flagella. J Clin Microbiol 6: 414–419 [PubMed].
    [Google Scholar]
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