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Abstract

A novel thermophilic, obligately anaerobic bacterium, strain Cel2f, was isolated from a cellulolytic community enriched from coastal marine sediment. Cells were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Optimal growth temperature and pH of strain Cel2f were 55 °C and pH 7.0, respectively. NaCl was essential for the growth of strain Cel2f and the strain showed enhanced growth in the presence of sea salt; the optimum sea salt concentration for growth was 7 % (w/v). Thiosulfate, sulfate and sulfite were potential electron acceptors. The major fatty acids of strain Cel2f were iso-C, C, and C. Polar lipid analysis indicated the presence of phosphatidylethanolamine and phosphatidylglycerol. Strain Cel2f contained menaquinone MK-7 as the isoprenoid quinone, and the DNA G+C content was 31.3 mol%. Phylogenetic analysis revealed that the nearest relative of strain Cel2f was BM with 93.8 % 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain Cel2f represents a novel species of genus , for which the name sp. nov. is proposed. The type strain is Cel2f ( = JCM 30480 = CGMCC 1.5200).

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2015-09-01
2021-03-08
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