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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_9

sp. nov., a thermophilic actinomycete isolated from mushroom compost Free

Abstract

A novel thermophilic actinomycete, designated strain CD-1, was isolated from mushroom compost in Nanning, Guangxi province, China. The strain grew at 37–55 °C (optimum 45–50 °C), pH 6.0–11.0 (optimum pH 7.0–9.0) and with 0–2.0  % NaCl (optimum 0–1.0  %), formed well-developed white aerial mycelium and pale-yellow vegetative mycelium, and single endospores (0.8–1.0 μm diameter) were borne on long sporophores (2–3 μm length). The endospores were spherical-polyhedron in shape with smooth surface. Based on its phenotypic and phylogenetic characteristics, strain CD-1 is affiliated to the genus . It contained -diaminopimelic acid as the diagnostic diamino acid; the whole-cell sugars were ribose and glucose. Major fatty acids were iso-C, C, anteiso-C and iso-C. MK-7 was the predominant menaquinone. The polar phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylethanolamine containing hydroxylated fatty acids, ninhydrin-positive glycophospholipid, an unknown phospholipid and glycolipids. The G+C content of the genomic DNA was 48.8  %. 16S rRNA gene sequence analysis showed that the organism was closely related to YIM 77831 (95.69  % sequence similarity), H-18 (95.49  %), KCTC 3666 (95.05  %), KCTC 9076 (95.01  %) and JCM 3312 (94.55  %). Levels of DNA–DNA relatedness between strain CD-1 and JCM 18059, DSM 45914, JCM 8091, JCM 3162 and JCM 3312 were low (22.8, 33.3, 24.7, 29.4 and 30.0  %, respectively). A battery of phenotypic, genotypic and DNA–DNA relatedness data indicated that strain CD-1 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CD-1 ( = ATCC BAA-2630 = CGMCC 4.7156).

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2015-09-01
2024-04-19
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Thermoactinomyces guangxiensis sp. nov., a thermophilic actinomycete isolated from mushroom compost
Int J Syst Evol Microbiol 65, 2859 (2015); https://doi.org/10.1099/ijs.0.000342
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