1887

Abstract

A Gram-staining-negative, facultatively aerobic bacterium, strain XCD-X85, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9  %, w/v) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85 were non-endospore-forming rods, 0.4–0.6 μm wide and 1.0–1.6 μm long, and motile by means of a single polar flagellum. Strain XCD-X85 was catalase- and oxidase-positive. Growth was observed in the presence of 0–12.0  % (w/v) NaCl (optimum, 1.0–2.0  %) and at 4–35 °C (optimum, 25–30 °C) and pH 6.5–10.5 (optimum, pH 8.0–8.5). Strain XCD-X85 contained (>10  %) summed feature 8 (Cω7 and/or Cω6), C, C and summed feature 3 (Cω7 and/or Cω6) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (Q-9). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 57.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X85 was associated with the genus , and showed highest 16S rRNA gene sequence similarities to CL-AP6 (99.0  %) and BZ93 (96.8  %). DNA–DNA relatedness of strain XCD-X85 to JCM 15562 was 19 ± 1  %. On the basis of the data presented above, it is concluded that strain XCD-X85 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XCD-X85 ( = CGMCC 1.12482 = JCM 19469).

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2015-09-01
2019-12-15
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References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J.. ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Anzai Y., Kim H., Park J.Y., Wakabayashi H., Oyaizu H.. ( 2000;). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50: 1563–1589 [CrossRef] [PubMed].
    [Google Scholar]
  3. Collins M.D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;.
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  5. Dong X.Z., Cai M.Y.. ( 2001;). Determinative Manual for Routine Bacteriology Beijing: Scientific Press; (English translation).
    [Google Scholar]
  6. Eguchi M., Nishikawa T., Macdonald K., Cavicchioli R., Gottschal J.C., Kjelleberg S.. ( 1996;). Responses to stress and nutrient availability by the marine ultramicrobacterium Sphingomonas sp. strain RB2256. Appl Environ Microbiol 62: 1287–1294 [PubMed].
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Fitch W.M.. ( 1971;). Toward defining course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  9. Gerhardt P., Murray R.G.E., Wood W.A., Krieg N.R.(editors). ( 1994;). Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  10. Huss V.A., Festl H., Schleifer K.H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  11. Hwang C.Y., Zhang G.I., Kang S.H., Kim H.J., Cho B.C.. ( 2009;). Pseudomonas pelagia sp. nov. isolated from a culture of the Antarctic green alga Pyramimonas gelidicola. Int J Syst Evol Microbiol 59: 3019–3024 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kates M.. ( 1986;). Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;.
    [Google Scholar]
  13. Kim K.H., Roh S.W., Chang H.W., Nam Y.D., Yoon J.H., Jeon C.O., Oh H.M., Bae J.W.. ( 2009;). Pseudomonas sabulinigri sp. nov. isolated from black beach sand. Int J Syst Evol Microbiol 59: 38–41 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution Cambridge: [CrossRef] Cambridge University Press;.
    [Google Scholar]
  16. Lai Q., Shao Z.. ( 2008;). Pseudomonas xiamenensis sp. nov. a denitrifying bacterium isolated from activated sludge. Int J Syst Evol Microbiol 58: 1911–1915 [CrossRef] [PubMed].
    [Google Scholar]
  17. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., other authors. ( 2007;). Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948 [CrossRef] [PubMed].
    [Google Scholar]
  18. Lin S.Y., Hameed A., Liu Y.C., Hsu Y.H., Lai W.A., Young C.C.. ( 2013;). Pseudomonas formosensis sp. nov. a gamma-proteobacteria isolated from food-waste compost in Taiwan. Int J Syst Evol Microbiol 63: 3168–3174 [CrossRef] [PubMed].
    [Google Scholar]
  19. Liu M., Luo X., Zhang L., Dai J., Wang Y., Tang Y., Li J., Sun T., Fang C.. ( 2009;). Pseudomonas xinjiangensis sp. nov. a moderately thermotolerant bacterium isolated from desert sand. Int J Syst Evol Microbiol 59: 1286–1289 [CrossRef] [PubMed].
    [Google Scholar]
  20. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  21. Migula W.. ( 1894;). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1: 235–238 (in German).
    [Google Scholar]
  22. Moore E.R.B., Mau M., Arnscheidt A., Bottger E.C., Hutson R.A., Collins M.D., VandePeer Y., DeWachter R., Timmis K.N.. ( 1996;). The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Syst Appl Microbiol 19: 478–492 [CrossRef].
    [Google Scholar]
  23. Mulet M., Lalucat J., García-Valdés E.. ( 2010;). DNA sequence-based analysis of the Pseudomonas species. Environ Microbiol 12: 1513–1530 [PubMed].
    [Google Scholar]
  24. Nokhal T.H., Schlegel H.G.. ( 1983;). Taxonomic study of Paracoccus denitrificans. Int J Syst Bacteriol 33: 26–37 [CrossRef].
    [Google Scholar]
  25. Oyaizu H., Komagata K.. ( 1983;). Grouping of Pseudomonas species on the basis of cellular fatty-acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty-acids. J Gen Appl Microbiol 29: 17–40 [CrossRef].
    [Google Scholar]
  26. Palleroni N.J.. ( 1984;). Genus I. Pseudomonas Migula 1894, 237AL. . In Bergey's Manual of Systematic Bacteriologyvol. 1, pp. 141–199. Edited by Krieg N. R., Holt J. G.. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  27. Palleroni N.J.. ( 2005;). Genus I. Pseudomonas Migula 1894, 237AL. . In Bergey's Manual of Systematic Bacteriology, pp. 323–379. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M..vol. 2, part B, 2nd edn. New York: Springer;.
    [Google Scholar]
  28. Pascual J., Lucena T., Ruvira M.A., Giordano A., Gambacorta A., Garay E., Arahal D.R., Pujalte M.J., Macián M.C.. ( 2012;). Pseudomonas litoralis sp. nov. isolated from Mediterranean seawater. Int J Syst Evol Microbiol 62: 438–444 [CrossRef] [PubMed].
    [Google Scholar]
  29. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  30. Sneath P.H.A., Stevens M., Sackin M.J.. ( 1981;). Numerical taxonomy of Pseudomonas based on published records of substrate utilization. Antonie van Leeuwenhoek 47: 423–448 [CrossRef] [PubMed].
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  32. Vancanneyt M., Witt S., Abraham W.R., Kersters K., Fredrickson H.L.. ( 1996;). Fatty acid content in whole-cell hydrolysates and phospholipid fractions of pseudomonads: a taxonomic evaluation. Syst Appl Microbiol 19: 528–540 [CrossRef].
    [Google Scholar]
  33. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  34. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697–703 [PubMed].
    [Google Scholar]
  35. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  36. Yang G., Han L., Wen J., Zhou S.. ( 2013;). Pseudomonas guangdongensis sp. nov. isolated from an electroactive biofilm, and emended description of the genus Pseudomonas Migula 1894. Int J Syst Evol Microbiol 63: 4599–4605 [CrossRef] [PubMed].
    [Google Scholar]
  37. Zhang D.C., Liu H.C., Zhou Y.G., Schinner F., Margesin R.. ( 2011;). Pseudomonas bauzanensis sp. nov. isolated from soil. Int J Syst Evol Microbiol 61: 2333–2337 [CrossRef] [PubMed].
    [Google Scholar]
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