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Volume 65, Issue Pt_9

sp. nov., isolated from a salt lake Free

Abstract

A Gram-staining-negative, facultatively aerobic bacterium, strain XCD-X85, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9  %, w/v) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85 were non-endospore-forming rods, 0.4–0.6 μm wide and 1.0–1.6 μm long, and motile by means of a single polar flagellum. Strain XCD-X85 was catalase- and oxidase-positive. Growth was observed in the presence of 0–12.0  % (w/v) NaCl (optimum, 1.0–2.0  %) and at 4–35 °C (optimum, 25–30 °C) and pH 6.5–10.5 (optimum, pH 8.0–8.5). Strain XCD-X85 contained (>10  %) summed feature 8 (Cω7 and/or Cω6), C, C and summed feature 3 (Cω7 and/or Cω6) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (Q-9). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 57.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X85 was associated with the genus , and showed highest 16S rRNA gene sequence similarities to CL-AP6 (99.0  %) and BZ93 (96.8  %). DNA–DNA relatedness of strain XCD-X85 to JCM 15562 was 19 ± 1  %. On the basis of the data presented above, it is concluded that strain XCD-X85 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XCD-X85 ( = CGMCC 1.12482 = JCM 19469).

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2015-09-01
2024-04-19
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Pseudomonas salina sp. nov., isolated from a salt lake
Int J Syst Evol Microbiol 65, 2846 (2015); https://doi.org/10.1099/ijs.0.000341
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