1887

Abstract

A Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain JM-1396) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (, cultivar ‘DES-119’) grown at the Plant Breeding Unit at the E. V. Smith Research Center in Tallassee (Macon county), AL, USA. 16S rRNA gene sequence analysis of strain JM-1396 showed high sequence similarity values to the type strains of (both 98.6 %) and (98.5 %); sequence similarities to all other type strains of species of the genus were below 98.3 %. DNA–DNA pairing experiments of the DNA of strain JM-1396 and SM117, SM16 and DSM 25411 showed low relatedness values of 8 % (reciprocal 7 %), 24 % (reciprocal 26 %) and 19 % (reciprocal 25 %), respectively. Ubiquinone Q-10 was detected as the dominant quinone; the fatty acids Cω7 (71.0 %) and the typical 2-hydroxy fatty acid, C 2-OH (11.7 %), were detected as typical components. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. The polyamine pattern contained the major compound spermidine and only minor amounts of other polyamines. All these data revealed that strain JM-1396 represents a novel species of the genus For this reason we propose the name sp. nov. with the type strain JM-1396 ( = LMG 28605 = CCM 8569 = CIP 110884).

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2015-09-01
2019-10-17
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