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Volume 65, Issue Pt_9

sp. nov., a halophilic bacterium isolated from a salt lake Free

Abstract

A Gram-staining-negative bacterium, strain XCD-X12, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X12 were non-spore-forming rods, 0.4–0.7 μm wide, 2.1–3.2 μm long and motile with a single polar flagellum. Strain XCD-X12 was strictly aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–20.0 % (w/v) NaCl (optimum, 4.0–8.0 %), at 4–35 °C (optimum, 30 °C) and at pH 6.5–10.5 (optimum, pH 8.5). It contained Q-9 as the predominant respiratory quinone. The major fatty acids (>10.0 %) were C, Cω9 and Cω9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and an uncharacterized aminophospholipid. The DNA G+C content was 55.6 mol% ( ). Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X12 was associated with the genus , and showed the highest 16S rRNA gene sequence similarity to ATCC 49840 (97.4 %), FB1 (96.8 %), KMM 3809 (96.8 %) and ZS2-30 (96.7 %). DNA–DNA relatedness of strain XCD-X12 to CGMCC 1.7683 was 34 ± 5 %. Based on these data, it is concluded that strain XCD-X12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XCD-X12 ( = CGMCC 1.12481 = JCM 30472).

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2015-09-01
2024-04-25
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Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake
Int J Syst Evol Microbiol 65, 2838 (2015); https://doi.org/10.1099/ijs.0.000338
/content/journal/ijsem/10.1099/ijs.0.000338
/content/journal/ijsem/10.1099/ijs.0.000338
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