1887

Abstract

Two novel Gram-staining-positive bacteria, designated DCY60 and DCY90, were isolated from soil of a ginseng field in the Republic of Korea. 16S rRNA gene sequence comparisons showed the two novel strains were closely related to members of the genus with greatest similarity to KCTC 33009 (98.8 and 98.4 % for DCY60 and DCY90, respectively). The predominant menaquinones present were MK-11 and MK-12. The major fatty acids were anteiso-C and summed feature 8 containing Cω7 and/or Cω6. The DNA G+C contents of strains DCY60 and DCY90 were 62.8 and 66.8 mol%, respectively. The peptidoglycan of both strains contained the amino acids ornithine, 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. The cell-wall sugars of strain DCY60 comprised glucose, galactose, rhamnose and xylose, while strain DCY90 contained glucose, galactose, rhamnose and ribose. The major polar lipids of both strains were phosphatidylglycerol, an unidentified glycolipid, and an unknown phospholipid. On the basis of the phenotypic analysis strains DCY60 and DCY90 represent novel species of the genus for which names sp. nov. (type strain DCY60 = KCTC 33520 = JCM 30079) and sp. nov. (type strain DCY90 = KCTC 33521 = JCM 30080) are proposed.

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2015-08-01
2019-10-16
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