1887

Abstract

A bacterial strain, YYQ-30, isolated from a mixed water–sand–sediment sample collected from a terrestrial spring located in Dunhuang, China, was characterized with respect to its morphology, physiology and taxonomy. Cells of the strain were Gram-stain-negative, aerobic, oxidase- and catalase-positive, non-flagellated, oval to rod-shaped (0.5–1.0 μm wide and 1.1–6.6 μm long) and divided by binary fission. Growth was observed in the presence of 0–10.0 % (w/v) NaCl with optimal growth at 0–3.0 %, at pH 6.0–9.0 (optimum pH 7.0–8.5) and at 10–45 °C (optimum 30–37 °C). The isolate could reduce nitrate to nitrite and hydrolyse aesculin and gelatin (weakly), but was unable to degrade Tween 80 or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YYQ-30 belongs to the family and forms a distinct lineage with the type strain of and forms a branch within a cluster constituted by the type strains of species of the genera , , , and ; levels of 16S rRNA gene sequence similarity between strain YYQ-30 and members of related genera ranged from 94.1 to 89.7  %. Strain YYQ-30 contained Q-10 as the predominant ubiquinone and summed feature 8 (Cω7 and/or Cω6; 70.0  %), C (9.5  %), summed feature 2 (one or more of C 3-OH, iso-C I and C aldehyde; 6.9  %) and 11-methyl Cω7 (6.0  %) as the principal fatty acids. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, two unidentified aminolipids and five unknown lipids. The gene was detected. The G+C content of the genomic DNA was 71.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain YYQ-30 is considered to represent a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is YYQ-30 ( = LMG 27418 = MCCC 1A06483).

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2015-09-01
2022-01-26
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References

  1. Albuquerque L., Santos J., Travassos P., Nobre M.F., Rainey F.A., Wait R., Empadinhas N., Silva M.T., da Costa M.S. ( 2002;). Albidovulum inexpectatum gen. nov., sp. nov., a nonphotosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to members of the photosynthetic genus Rhodovulum . Appl Environ Microbiol 68 42664273 [View Article] [PubMed] .
    [Google Scholar]
  2. Anil Kumar P., Srinivas T.N., Sasikala Ch., Ramana Ch.V. ( 2007;). Rhodobacter changlensis sp. nov., a psychrotolerant, phototrophic alphaproteobacterium from the Himalayas of India. Int J Syst Evol Microbiol 57 25682571 [View Article] [PubMed] .
    [Google Scholar]
  3. Ausubel F., Brent R., Kingston R., Moore D., Seidman J., Smith J., Struhl K. ( 1995). Short Protocols in Molecular Biology, 3rd edn New York: Wiley;.
    [Google Scholar]
  4. Béjà O., Suzuki M.T., Heidelberg J.F., Nelson W.C., Preston C.M., Hamada T., Eisen J.A., Fraser C.M., DeLong E.F. ( 2002;). Unsuspected diversity among marine aerobic anoxygenic phototrophs. Nature 415 630633 [View Article] [PubMed] .
    [Google Scholar]
  5. Biebl H., Allgaier M., Tindall B.J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. ( 2005;). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. Int J Syst Evol Microbiol 55 10891096 [View Article] [PubMed] .
    [Google Scholar]
  6. Boettcher K.J., Geaghan K.K., Maloy A.P., Barber B.J. ( 2005;). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55 15311537 [View Article] [PubMed] .
    [Google Scholar]
  7. Chakravarthy S.K., Sucharitha K., Sasikala Ch., Ramana Ch.V. ( 2009;). Rhodovulum lacipunicei sp. nov., an obligate sulfide-demanding phototrophic alphaproteobacterium isolated from a purple pond in India. Int J Syst Evol Microbiol 59 16151619 [View Article] [PubMed] .
    [Google Scholar]
  8. Chen W.-M., Cho N.-T., Huang W.-C., Young C.-C., Sheu S.-Y. ( 2013;). Description of Gemmobacter fontiphilus sp. nov., isolated from a freshwater spring, reclassification of Catellibacterium nectariphilum as Gemmobacter nectariphilus comb. nov., Catellibacterium changlense as Gemmobacter changlensis comb. nov., Catellibacterium aquatile as Gemmobacter aquaticus nom. nov., Catellibacterium caeni as Gemmobacter caeni comb. nov., Catellibacterium nanjingense as Gemmobacter nanjingensis comb. nov., and emended description of the genus Gemmobacter and of Gemmobacter aquatilis . Int J Syst Evol Microbiol 63 470478 [View Article] [PubMed] .
    [Google Scholar]
  9. Choi E.J., Lee H.J., Kim J.M., Jeon C.O. ( 2013;). Roseovarius lutimaris sp. nov., isolated from a marine tidal flat. Int J Syst Evol Microbiol 63 38353840 [View Article] [PubMed] .
    [Google Scholar]
  10. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed] .
    [Google Scholar]
  11. Greub G., Raoult D. ( 2003;). Rhodobacter massiliensis sp. nov., a new amoebae-resistant species isolated from the nose of a patient. Res Microbiol 154 631635 [View Article] [PubMed] .
    [Google Scholar]
  12. Harwati T.U., Kasai Y., Kodama Y., Susilaningsih D., Watanabe K. ( 2009;). Tropicimonas isoalkanivorans gen. nov., sp. nov., a branched-alkane-degrading bacterium isolated from Semarang Port in Indonesia. Int J Syst Evol Microbiol 59 388391 [View Article] [PubMed] .
    [Google Scholar]
  13. Helsel L.O., Hollis D., Steigerwalt A.G., Morey R.E., Jordan J., Aye T., Radosevic J., Jannat-Khah D., Thiry D., other authors. ( 2007;). Identification of “Haematobacter,” a new genus of aerobic Gram-negative rods isolated from clinical specimens, and reclassification of Rhodobacter massiliensis as “Haematobacter massiliensis comb. nov”. J Clin Microbiol 45 12381243 [View Article] [PubMed] .
    [Google Scholar]
  14. Hiraishi A., Ueda Y. ( 1994;). Intrageneric structure of the genus Rhodobacter: transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov. Int J Syst Bacteriol 44 1523 [View Article].
    [Google Scholar]
  15. Imhoff J., Trüper H., Pfennig N. ( 1984;). Rearrangement of the species and genera of the phototrophic “purple nonsulfur bacteria”. Int J Syst Bacteriol 34 340343 [View Article].
    [Google Scholar]
  16. Jung Y.-T., Park S., Yoon J.-H. ( 2012;). Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula. Antonie van Leeuwenhoek 102 141148 [View Article] [PubMed] .
    [Google Scholar]
  17. Kates M. ( 1986). Techniques of Lipidology Amsterdam: Elsevier;.
    [Google Scholar]
  18. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012a;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  19. Kim Y.-O., Kong H.J., Park S., Kang S.-J., Kim W.-J., Kim K.-K., Oh T.-K., Yoon J.-H. ( 2012b;). Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi . Int J Syst Evol Microbiol 62 931936 [View Article] [PubMed] .
    [Google Scholar]
  20. Labrenz M., Collins M.D., Lawson P.A., Tindall B.J., Schumann P., Hirsch P. ( 1999;). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49 137147 [View Article] [PubMed] .
    [Google Scholar]
  21. Lai Q., Yuan J., Gu L., Shao Z. ( 2009a;). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59 12781281 [View Article] [PubMed] .
    [Google Scholar]
  22. Lai Q., Yuan J., Wu C., Shao Z. ( 2009b;). Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59 17331737 [View Article] [PubMed] .
    [Google Scholar]
  23. Lai Q., Zhong H., Wang J., Yuan J., Sun F., Wang L., Zheng T., Shao Z. ( 2011;). Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean. Int J Syst Evol Microbiol 61 20402044 [View Article] [PubMed] .
    [Google Scholar]
  24. Lakshmi K.V., Sasikala C., Venkata Ramana V., Ramaprasad E.V., Venkata Ramana C. ( 2011;). Rhodovulum phaeolacus sp. nov. a phototrophic alphaproteobacterium isolated from a brown pond. J Gen Appl Microbiol 57 145151 [View Article] [PubMed] .
    [Google Scholar]
  25. Li G.-W., Zhang X.-Y., Wang C.-S., Zhang Y.-J., Xu X.-W., Liu C., Li H., Liu A., Xu Z., other authors. ( 2013a;). Albimonas pacifica sp. nov., isolated from seawater of the Pacific, and emended description of the genus Albimonas . Int J Syst Evol Microbiol 63 35973601 [View Article] [PubMed] .
    [Google Scholar]
  26. Li Z., Zhao R., Ji S., Shi X., Zhang X.-H. ( 2013b;). Roseovarius marisflavi sp. nov., isolated from an amphioxus breeding zone in the coastal region of the Yellow Sea, China. Antonie van Leeuwenhoek 104 413421 [View Article] [PubMed] .
    [Google Scholar]
  27. Lim J.-M., Jeon C.O., Jang H.H., Park D.-J., Shin Y.K., Yeo S.-H., Kim C.-J. ( 2008;). Albimonas donghaensis gen. nov., sp. nov., a non-photosynthetic member of the class Alphaproteobacteria isolated from seawater. Int J Syst Evol Microbiol 58 282285 [View Article] [PubMed] .
    [Google Scholar]
  28. Liu C., Shao Z. ( 2005;). Alcanivorax dieselolei sp. nov. a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55 11811186 [View Article] [PubMed] .
    [Google Scholar]
  29. Liu Y., Xu C.-J., Jiang J.-T., Liu Y.-H., Song X.-F., Li H., Liu Z.-P. ( 2010;). Catellibacterium aquatile sp. nov., isolated from fresh water, and emended description of the genus Catellibacterium Tanaka et al. 2004. Int J Syst Evol Microbiol 60 20272031 [View Article] [PubMed] .
    [Google Scholar]
  30. Liu J.-J., Zhang X.-Q., Chi F.-T., Pan J., Sun C., Wu M. ( 2014;). Gemmobacter megaterium sp. nov., isolated from coastal planktonic seaweeds. Int J Syst Evol Microbiol 64 6671 [View Article] [PubMed] .
    [Google Scholar]
  31. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+ cytosine of DNA. J Chromatogr A 479 297306 [View Article] [PubMed] .
    [Google Scholar]
  32. Nupur P., Srinivas T.N., Takaichi S., Anil Kumar P. ( 2014;). Rhodovulum mangrovi sp. nov. a phototrophic alphaproteobacterium isolated from a mangrove forest sediment sample. Int J Syst Evol Microbiol 64 31683173 [View Article] [PubMed] .
    [Google Scholar]
  33. Oh Y.-S., Lim H.-J., Cha I.-T., Im W.-T., Yoo J.-S., Kang U.G., Rhee S.-K., Roh D.-H. ( 2009;). Roseovarius halotolerans sp. nov., isolated from deep seawater. Int J Syst Evol Microbiol 59 27182723 [View Article] [PubMed] .
    [Google Scholar]
  34. Oh K.-H., Choi W.-C., Jung Y.-T., Kang S.-J., Oh T.-K., Yoon J.-H. ( 2012;). Tropicimonas aquimaris sp. nov., isolated from seawater, and emended description of the genus Tropicimonas Harwati et al. 2009. Int J Syst Evol Microbiol 62 688692 [View Article] [PubMed] .
    [Google Scholar]
  35. Park S., Yoon J.-H. ( 2013;). Roseovarius sediminilitoris sp. nov., isolated from seashore sediment. Int J Syst Evol Microbiol 63 17411745 [View Article] [PubMed] .
    [Google Scholar]
  36. Rothe B., Fischer A., Hirsch P., Sittig M., Stackebrandt E. ( 1987;). The phylogenetic position of the budding bacteria Blastobacter aggregatus and Gemmobacter aquatilis gen., nov. sp. nov. Arch Microbiol 147 9299 [View Article].
    [Google Scholar]
  37. Rzhetsky A., Nei M. ( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35 367375 [View Article] [PubMed] .
    [Google Scholar]
  38. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  39. Sambrook J., Fritsch E.F., Maniatis T. ( 1989). Molecular Cloning New York: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  40. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI;.
    [Google Scholar]
  41. Shin N.-R., Roh S.W., Kim M.-S., Yun B., Whon T.W., Kim Y.-O., Bae J.-W. ( 2012;). Tropicimonas sediminicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 62 24242429 [View Article] [PubMed] .
    [Google Scholar]
  42. Srinivas A., Rahul K., Ramaprasad E.V., Sasikala Ch., Ramana Ch.V. ( 2012;). Rhodovulum bhavnagarense sp. nov. a phototrophic alphaproteobacterium isolated from a pink pond. Int J Syst Evol Microbiol 62 25282532 [View Article] [PubMed] .
    [Google Scholar]
  43. Srinivas A., Vinay Kumar B., Divya Sree B., Tushar L., Sasikala Ch., Ramana Ch.V. ( 2014;). Rhodovulum salis sp. nov. and Rhodovulum viride sp. nov. phototrophic alphaproteobacteria isolated from marine habitats. Int J Syst Evol Microbiol 64 957962 [View Article] [PubMed] .
    [Google Scholar]
  44. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed] .
    [Google Scholar]
  45. Tanaka Y., Hanada S., Manome A., Tsuchida T., Kurane R., Nakamura K., Kamagata Y. ( 2004;). Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth. Int J Syst Evol Microbiol 54 955959 [View Article] [PubMed] .
    [Google Scholar]
  46. Wang D., Liu H., Zheng S., Wang G. ( 2014;). Paenirhodobacter enshiensis gen. nov., sp. nov., a non-photosynthetic bacterium isolated from soil, and emended descriptions of the genera Rhodobacter and Haematobacter . Int J Syst Evol Microbiol 64 551558 [View Article] [PubMed] .
    [Google Scholar]
  47. Yarza P., Yilmaz P., Pruesse E., Glöckner F.O., Ludwig W., Schleifer K.-H., Whitman W.B., Euzéby J., Amann R., Rosselló-Móra R. ( 2014;). Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 12 635645 [View Article] [PubMed] .
    [Google Scholar]
  48. Yin D., Xiao J., Ao J., Ai C., Chen X. ( 2012;). Albidovulum xiamenense sp. nov. a moderately thermophilic bacterium from a terrestrial hot spring. Int J Syst Evol Microbiol 62 16091612 [View Article] [PubMed] .
    [Google Scholar]
  49. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2008;). Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 58 11981202 [View Article] [PubMed] .
    [Google Scholar]
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