1887

Abstract

A bacterial strain, YYQ-30, isolated from a mixed water–sand–sediment sample collected from a terrestrial spring located in Dunhuang, China, was characterized with respect to its morphology, physiology and taxonomy. Cells of the strain were Gram-stain-negative, aerobic, oxidase- and catalase-positive, non-flagellated, oval to rod-shaped (0.5–1.0 μm wide and 1.1–6.6 μm long) and divided by binary fission. Growth was observed in the presence of 0–10.0 % (w/v) NaCl with optimal growth at 0–3.0 %, at pH 6.0–9.0 (optimum pH 7.0–8.5) and at 10–45 °C (optimum 30–37 °C). The isolate could reduce nitrate to nitrite and hydrolyse aesculin and gelatin (weakly), but was unable to degrade Tween 80 or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YYQ-30 belongs to the family and forms a distinct lineage with the type strain of and forms a branch within a cluster constituted by the type strains of species of the genera , , , and ; levels of 16S rRNA gene sequence similarity between strain YYQ-30 and members of related genera ranged from 94.1 to 89.7  %. Strain YYQ-30 contained Q-10 as the predominant ubiquinone and summed feature 8 (Cω7 and/or Cω6; 70.0  %), C (9.5  %), summed feature 2 (one or more of C 3-OH, iso-C I and C aldehyde; 6.9  %) and 11-methyl Cω7 (6.0  %) as the principal fatty acids. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, two unidentified aminolipids and five unknown lipids. The gene was detected. The G+C content of the genomic DNA was 71.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain YYQ-30 is considered to represent a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is YYQ-30 ( = LMG 27418 = MCCC 1A06483).

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2015-09-01
2019-10-14
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