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Abstract

A novel aerobic, Gram-staining-negative bacterium, designated strain LAM-WHM-ZC, was isolated from coastal sediment samples from the Bohai Sea, near Yantai, China. Cells of LAM-WHM-ZC were non-motile, short-rod- or coccoid-shaped. The temperature and pH ranges for growth were 4–40  °C (optimum: 20–33  °C) and pH 5–9 (optimum: pH 7.5). The strain did not require NaCl for growth but tolerated up to 10 % NaCl (w/v). The major fatty acids of strain LAM-WHM-ZC were summed feature 3, C, C, summed feature 2 and summed feature 8. The predominant respiratory quinone was ubiquinone Q-8. The main polar lipids were diphosphatidyglycerol, phosphatigylethanolamine, phosphatidyglycerol, one phospholipid and four unidentified glycolipids. The DNA G+C content was 59.3 mol% as determined by the melting temperature ( ) method. Analysis of the 16S rRNA gene sequence indicated that the isolate represented a member of the genus and was closely related to KCTC 23013, DSM 15406, KACC 15117 and KCTC 12522 with 97.7 %, 97.1 %, 96.6 % and 96.6 % sequence similarity, respectively. The DNA–DNA hybridization values between strain LAM-WHM-ZC and the four reference strains were 47.4 ± 2.8 %, 33.5 ± 2.2 %, 28.4 ± 1.8 % and 13.7 ± 0.8 %, respectively. Based on its phenotypic and genotypic properties, strain LAM-WHM-ZC is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LAM-WHM-ZC ( = ACCC 06516 = JCM 30466).

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2015-09-01
2020-01-22
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