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Volume 65, Issue Pt_8

gen. nov., sp. nov., a mesophilic member of the family isolated from an agricultural soil sample Free

Abstract

A mesophilic strain, designed MC 210, was isolated from an agricultural soil sample from Pyongyang, Democratic People's Republic of Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on PYI medium, and no diffusible pigments were produced. The optimum temperature for growth was 37 °C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-reaction-positive and non-motile and formed endospores on the aerial mycelium. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MC 210 belongs to the family . Strain MC 210 showed 16S rRNA gene sequence similarities of 92.90 and 92.54  % to the type strains of and , respectively. The cell wall of strain MC 210 contained -diaminopimelic acid, glutamic acid and alanine as the diagnostic amino acids, and whole-cell hydrolysates contained glucose, arabinose and galactose. Strain MC 210 contained anteiso-C, iso-C, C, anteiso-C, C and iso-C as the major cellular fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown aminophospholipids, an unknown aminolipid, three unknown phospholipids and five unknown polar lipids. The predominant menaquinone was MK-7.The G+C content of the genomic DNA was 42.1 mol%. On the basis of our phylogenetic, physiological and chemotaxonomic data, strain MC 210 is considered to represent a novel genus and species, for which we propose the name gen. nov., sp. nov., in the family . The type strain of is MC 210 (CCTCC AA 2013021 = NRRL B-59118).

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2015-08-01
2024-04-25
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References

  1. Addou A.N., Schumann P., Spröer C., Hacene H., Cayol J.L., Fardeau M.L. ( 2012;). Melghirimyces algeriensis gen. nov., sp. nov., a member of the family Thermoactinomycetaceae, isolated from a salt lake. Int J Syst Evol Microbiol 62 1491–1498 [View Article] [PubMed].
     [Google Scholar]
  2. Atlas R.M. ( 1993). Handbook of Microbiological Media, pp. 126–127. Edited by Parks L. C. Boca Raton, FL: CRC Press;.
     [Google Scholar]
  3. Buss S.N., Cole J.A., Hannett G.E., Nazarian E.J., Nazarian L., Coorevits A., Van Landschoot A., De Vos P., Schumann P., authors other. ( 2013;). Hazenella coriacea gen. nov., sp. nov., isolated from clinical specimens. Int J Syst Evol Microbiol 63 4087–4093 [View Article] [PubMed].
     [Google Scholar]
  4. Chen J.J., Lin L.B., Zhang L.L., Zhang J., Tang S.K., Wei Y.L., Li W.J. ( 2012;). Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 62 38–42 [View Article] [PubMed].
     [Google Scholar]
  5. Chun J., Lee J.H., Jung Y., Kim M., Kim S., Kim B.K., Lim Y.W. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57 2259–2261 [View Article] [PubMed].
     [Google Scholar]
  6. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221–230 [View Article] [PubMed].
     [Google Scholar]
  7. Cowan S.T., Steel K.J. ( 1965). Manual for the Identification of Medical Bacteria New York: Cambridge University Press;.
     [Google Scholar]
  8. Dong X.Z., Cai M.Y. ( 2001). Manual of Systematics and Identification of General Bacteria Beijing: Science Press;.
     [Google Scholar]
  9. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  10. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  11. Fitch W.M. ( 1971;). Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  12. Gerhardt P., Murray R.G.E., Wood W.A., Krieg N.R. ( 1994). Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  13. Gonzalez C., Gutierrez C., Ramirez C. ( 1978;). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24 710–715 [View Article] [PubMed].
     [Google Scholar]
  14. Hasegawa T., Takizawa M., Tanida S. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Microbiol 29 319–322 [View Article].
     [Google Scholar]
  15. Hatayama K., Shoun H., Ueda Y., Nakamura A. ( 2005;). Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family ‘Thermoactinomycetaceae’ isolated from compost. Int J Syst Evol Microbiol 55 2101–2104 [View Article] [PubMed].
     [Google Scholar]
  16. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  17. Kovacs N. ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178 703 [View Article] [PubMed].
     [Google Scholar]
  18. Kubica G.P., Kim T.H., Dunbar F.P. ( 1972;). Designation of strain H37Rv as the neotype of Mycobacterium tuberculosis . Int J Syst Bacteriol 22 99–106 [View Article].
     [Google Scholar]
  19. Lacey J. ( 1971;). Thermoactinomyces sacchari sp. nov., a thermophilic actinomycete causing bagassosis. J Gen Microbiol 66 327–338 [View Article] [PubMed].
     [Google Scholar]
  20. Lacey J., Cross T. ( 1989;). Genus Thermoactinomyces Tsiklinsky 1899, 501AL . . In Bergey's Manual of Systematic Bacteriology vol. 4, pp. 2574–2585. Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;.
     [Google Scholar]
  21. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
     [Google Scholar]
  22. Li J., Zhang G.T., Yang J., Tian X.P., Wang F.Z., Zhang C.S., Zhang S., Li W.J. ( 2012;). Marininema mesophilum gen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae . Int J Syst Evol Microbiol 62 1383–1388 [View Article] [PubMed].
     [Google Scholar]
  23. Lin Y.C., Uemori K., de Briel D.A., Arunpairojana V., Yokota A. ( 2004;). Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae . Int J Syst Evol Microbiol 54 1669–1676 [View Article] [PubMed].
     [Google Scholar]
  24. Locci R. ( 1989;). Streptomyces and related genera. . In Bergey's Manual of Systematic Bacteriology vol. 4, pp. 2451–2508. Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;.
     [Google Scholar]
  25. Matsuo Y., Katsuta A., Matsuda S., Shizuri Y., Yokota A., Kasai H. ( 2006;). Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov.. Int J Syst Evol Microbiol 56 2837–2842 [View Article] [PubMed].
     [Google Scholar]
  26. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  27. Moore D.D., Dowhan D. ( 1995;). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;.
     [Google Scholar]
  28. Nonomura H., Ohara Y. ( 1971;). Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes in soil. J Ferment Technol 49 895–903.
     [Google Scholar]
  29. Park D.J., Dastager S.G., Lee J.C., Yeo S.H., Yoon J.H., Kim C.J. ( 2007;). Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae . Int J Syst Evol Microbiol 57 2660–2664 [View Article] [PubMed].
     [Google Scholar]
  30. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  31. Sambrook J., Fritsch E.F., Maniatis T. ( 1989). Molecular Cloning: a Laboratory Manual , 2nd edn. NY: Cold Spring Harbor Laboratory;.
     [Google Scholar]
  32. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  33. Shirling E.B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313–340 [View Article].
     [Google Scholar]
  34. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 226–231 [PubMed].
     [Google Scholar]
  35. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  36. Tang S.K., Wang Y., Chen Y., Lou K., Cao L.L., Xu L.H., Li W.J. ( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59 2025–2031 [View Article] [PubMed].
     [Google Scholar]
  37. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  38. Tindall B.J. ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199–202 [View Article].
     [Google Scholar]
  39. Tsilinsky P. ( 1899;). [On the thermophilic moulds]. Ann Inst Pasteur (Paris) 13 500–505 (in French) .
     [Google Scholar]
  40. Tsubouchi T., Shimane Y., Mori K., Usui K., Hiraki T., Tame A., Uematsu K., Maruyama T., Hatada Y. ( 2013;). Polycladomyces abyssicola gen. nov., sp. nov., a thermophilic filamentous bacterium isolated from hemipelagic sediment. Int J Syst Evol Microbiol 63 1972–1981 [View Article] [PubMed].
     [Google Scholar]
  41. von Jan M., Riegger N., Pötter G., Schumann P., Verbarg S., Spröer C., Rohde M., Lauer B., Labeda D.P., Klenk H.P. et al. ( 2011;). Kroppenstedtia eburnea gen. nov., sp. nov., a thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009. Int J Syst Evol Microbiol 61 2304–2310 [View Article] [PubMed].
     [Google Scholar]
  42. Williams S.T., Goodfellow M., Alderson G. ( 1989;). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey's Manual of Systematic Bacteriology vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;.
     [Google Scholar]
  43. Xie C.H., Yokota A. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49 345–349 [View Article] [PubMed].
     [Google Scholar]
  44. Xu P., Li W.J., Tang S.K., Zhang Y.Q., Chen G.Z., Chen H.H., Xu L.H., Jiang C.L. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55 1149–1153 [View Article] [PubMed].
     [Google Scholar]
  45. Yao S., Liu Y., Zhang M., Zhang X., Li H., Zhao T., Xin C., Xu L., Zhang B., Cheng C. ( 2014;). Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu. Int J Syst Evol Microbiol 64 206–210 [View Article] [PubMed].
     [Google Scholar]
  46. Yassin A.F., Hupfer H., Klenk H.P., Siering C. et al. ( 2009;). Desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006. Int J Syst Evol Microbiol 59 454–459 [View Article] [PubMed].
     [Google Scholar]
  47. Yoon J.H., Kim I.G., Shin Y.K., Park Y.H. ( 2005;). Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella Thermoflavimicrobium Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 55 395–400 [View Article] [PubMed].
     [Google Scholar]
  48. Yu T.T., Zhang B.H., Yao J.C., Tang S.K., Zhou E.M., Yin Y.R., Wei D.Q., Ming H., Li W.J. ( 2012;). Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 102 711–718 [View Article] [PubMed].
     [Google Scholar]
  49. Zhang J., Tang S.K., Zhang Y.Q., Yu L.Y., Klenk H.P., Li W.J. ( 2010;). Laceyella tengchongensis sp. nov., a thermophile isolated from soil of a volcano. Int J Syst Evol Microbiol 60 2226–2230 [View Article] [PubMed].
     [Google Scholar]
  50. Zhou E.M., Yu T.T., Liu L., Ming H., Yin Y.R., Dong L., Tseng M., Nie G.X., Li W.J. ( 2014;). Geothermomicrobium terrae gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae . Int J Syst Evol Microbiol 64 2998–3004 [View Article] [PubMed].
     [Google Scholar]
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Risungbinella pyongyangensis gen. nov., sp. nov., a mesophilic member of the family Thermoactinomycetaceae isolated from an agricultural soil sample
Int J Syst Evol Microbiol 65, 2726 (2015); https://doi.org/10.1099/ijs.0.000328
/content/journal/ijsem/10.1099/ijs.0.000328
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