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Volume 65, Issue Pt_8

sp. nov., isolated from oyster Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and ovoid or rod-shaped bacterial strain, designated TYO-8, was isolated from an oyster collected from the South Sea in South Korea. Strain TYO-8 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain TYO-8 fell within the clade comprising the type strains of species of the genus , clustering coherently with the type strain of with a sequence similarity of 99.3 %. Strain TYO-8 exhibited 16S rRNA gene sequence similarity values of 95.3–97.5 % to the type strains of other species of the genus and of less than 92.9 % to the type strains of other species with validly published names. Strain TYO-8 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and iso-C G as the major fatty acids. The major polar lipids of strain TYO-8 were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain TYO-8 was 33.8 mol% and its DNA–DNA relatedness values with the type strains of , and were 13–27 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain TYO-8 is distinct from other species of the genus . On the basis of the data presented, strain TYO-8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TYO-8 ( = KCTC 42461 = NBRC 110923).

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2015-08-01
2024-04-19
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References

  1. Barrow G. I., Feltham R. K. A. (editors), ( 1993). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press; [View Article].
     [Google Scholar]
  2. Baumann P., Baumann L. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;.
     [Google Scholar]
  3. Bernardet J.-F. ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 4, pp. 106–111. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. New York: Springer;.
     [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 1049–1070 [View Article] [PubMed].
     [Google Scholar]
  5. Bowman J.P. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50 1861–1868 [PubMed]. [CrossRef]
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51 1997–2006 [View Article] [PubMed].
     [Google Scholar]
  7. Choi D.H., Cho B.C. ( 2006;). Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. Int J Syst Evol Microbiol 56 771–776 [View Article] [PubMed].
     [Google Scholar]
  8. Choi A., Yang S.-J., Cho J.-C. ( 2013;). Lutibacter flavus sp. nov., a marine bacterium isolated from a tidal flat sediment. Int J Syst Evol Microbiol 63 946–951 [View Article] [PubMed].
     [Google Scholar]
  9. Cohen-Bazire G., Sistrom W.R., Stanier R.Y. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49 25–68 [View Article] [PubMed].
     [Google Scholar]
  10. Embley T.M., Wait R. ( 1994;). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O'Donnell A. G. Chichester: John Wiley & Sons;.
     [Google Scholar]
  11. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in micro dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224–229 [View Article].
     [Google Scholar]
  12. Hunter I.S. ( 1985;). Gene cloning in Streptomyces . . In DNA cloning vol. 2, p. 32. Edited by Glover D. M. Oxford: IRL Press;.
     [Google Scholar]
  13. Komagata K., Suzuki K.-I. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161–207 [View Article].
     [Google Scholar]
  14. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 1–67 [View Article].
     [Google Scholar]
  15. Lee S.-Y., Lee M.-H., Oh T.-K., Yoon J.-H. ( 2012;). Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006. Int J Syst Evol Microbiol 62 420–424 [View Article] [PubMed].
     [Google Scholar]
  16. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  17. Park S., Kang S.-J., Oh T.-K., Yoon J.-H. ( 2010;). Lutibacter maritimus sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 60 610–614 [View Article] [PubMed].
     [Google Scholar]
  18. Park S.C., Choe H.N., Hwang Y.M., Baik K.S., Seong C.N. ( 2013;). Lutibacter agarilyticus sp. nov., a marine bacterium iso lated from shallow coastal seawater. Int J Syst Evol Microbiol 63 2678–2683 [View Article] [PubMed].
     [Google Scholar]
  19. Park S., Park D.-S., Bae K.S., Yoon J.-H. ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64 1378–1383 [View Article] [PubMed].
     [Google Scholar]
  20. Reichenbach H. ( 1992;). The order Cytophagales . . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn., pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;.
     [Google Scholar]
  21. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  22. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846–849 [View Article].
     [Google Scholar]
  23. Staley J.T. ( 1968;). Prosthecomicrobium Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95 1921–1942 [PubMed].
     [Google Scholar]
  24. Sung H.-R., Shin K.-S., Ghim S.-Y. ( 2015;). Lutibacter oricola sp. nov., a marine bacterium isolated from seawater. Int J Syst Evol Microbiol 65 485–490 [View Article] [PubMed].
     [Google Scholar]
  25. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125–128 [View Article].
     [Google Scholar]
  26. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
  27. Yoon J.-H., Lee S.T., Park Y.-H. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48 187–194 [View Article] [PubMed].
     [Google Scholar]
  28. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K.H., Park Y.-H. ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53 53–57 [View Article] [PubMed].
     [Google Scholar]
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Lutibacter crassostreae sp. nov., isolated from oyster
Int J Syst Evol Microbiol 65, 2689 (2015); https://doi.org/10.1099/ijs.0.000324
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