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Abstract

A novel endophytic actinomycete, designated strain EGI 60009, was isolated from the roots of F. collected from Xinjiang Province, north-west China. The isolate was able to grow in the presence of 0–9 % (w/v) NaCl. Strain EGI 60009 had particular morphological properties: the substrate mycelia fragmented into rod-like elements and aerial mycelia differentiated into short spore chains. -2, 6-Diaminopimelic acid was the cell-wall diamino acid and rhamnose, galactose and glucose were the cell-wall sugars. MK-9(H) was the predominant menaquinone. The major fatty acids of strain EGI 60009 were iso-C, anteiso-C, anteiso-C, iso-C, iso-C and I/anteiso-C B. Mycolic acids were absent. The DNA G+C content of strain EGI 60009 was 70.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain EGI 60009 belongs to the family and formed a distinct clade in the phylogenetic tree. 16S rRNA gene sequence similarities between strain EGI 60009 and other members of the genera and were 96.1–96.4 and 95.7–96.0 %, respectively. Based on these results and supported by morphological, physiological and chemotaxonomic data and numerous phenotypic differences, a novel species of a new genus, gen. nov., sp. nov., is proposed. The type strain of is EGI 60009 ( = KCTC 29657 = CPCC204078).

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2015-08-01
2021-10-21
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