1887

Abstract

A Gram-stain-negative, catalase- and oxidase-positive, facultatively aerobic bacterium, strain DSG-S6, was isolated from Dasugan Lake (salinity 3.1 %, w/w), China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S6 were non-spore-forming, slightly bent rods, and motile by means of a single polar flagellum. Growth occurred in the presence of 0–7.0  % (w/v) NaCl (optimum, 2.0  %), at 4–35 °C (optimum, 30 °C) and at pH 6.0–10.5 (optimum, pH 8.0–8.5). C, Cω7 and Cω7 and/or Cω6 were the major fatty acids. Six alleles of the 16S rRNA gene sharing 98.9–99.9  % similarity were detected in strain DSG-S6, which showed highest 16S rRNA gene sequence similarity to ATCC 35048 (97.7  %), then to LMG 19999 (97.6  %) and CIP 69.14 (96.8  %). Multilocus sequence analysis of four housekeeping genes and 16S rRNA genes clearly clustered it as a member of the Anguillarum clade. Mean DNA–DNA relatedness between strain DSG-S6 and NBRC 15629, CGMCC 1.12557 and JCM 21189 was 20.6 ± 2.3, 38.1 ± 3.5 and 24.2 ± 2.8  %, respectively. The DNA G+C content was 46.8 mol% ( ). Based on the data, it is concluded that strain DSG-S6 represents a novel species of the genus , for which the name Vibrio sp. nov. is proposed. The type strain is DSG-S6 ( = CGMCC 1.12427 = JCM 19265).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000316
2015-08-01
2021-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2653.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000316&mimeType=html&fmt=ahah

References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. ( 1990;). Basic local alignment search tool. J Mol Biol 215 403410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Beaz-Hidalgo R., Doce A., Balboa S., Barja J.L., Romalde J.L. ( 2010;). Aliivibrio finisterrensis sp. nov., isolated from Manila clam, Ruditapes philippinarum and emended description of the genus Aliivibrio . Int J Syst Evol Microbiol 60 223228 [CrossRef] [PubMed].
    [Google Scholar]
  3. Birkbeck T.H., Treasurer J.W. ( 2014;). Vibrio splendidus Vibrio ichthyoenteri Vibrio pacinii isolated from the digestive tract microflora of larval ballan wrasse, Labrus bergylta Ascanius, and goldsinny wrasse, Ctenolabrus rupestris (L.). J Fish Dis 37 6974 [CrossRef] [PubMed].
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [CrossRef] [PubMed].
    [Google Scholar]
  5. Dong X.Z., Cai M.Y. ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);.
    [Google Scholar]
  6. Farmer J.J., Janda J.M., Brenner F.W., Cameron D.N., Birkhead K.M. ( 2005;). Genus I. Vibrio Pacini 1854, 411AL . . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 2B, pp. 494546. Edited by Garrity G. M, Brenner D. J, Krieg N. R, Staley J. R. New York: Springer;.
    [Google Scholar]
  7. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Fitch W.M. ( 1971;). Toward defining course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [CrossRef].
    [Google Scholar]
  9. Gao Z.M., Xiao J., Wang X.N., Ruan L.W., Chen X.L., Zhang Y.Z. ( 2012;). Vibrio xiamenensis sp. nov., a cellulase-producing bacterium isolated from mangrove soil. Int J Syst Evol Microbiol 62 19581962 [CrossRef] [PubMed].
    [Google Scholar]
  10. Gomez-Gil B., Thompson F.L., Thompson C.C., Swings J. ( 2003;). Vibrio pacinii sp. nov., from cultured aquatic organisms. Int J Syst Evol Microbiol 53 15691573 [CrossRef] [PubMed].
    [Google Scholar]
  11. Gomez-Gil B., Thompson F.L., Thompson C.C., Garcia-Gasca A., Roque A., Swings J. ( 2004;). Vibrio hispanicus sp. nov., isolated from Artemia sp. and sea water in Spain. Int J Syst Evol Microbiol 54 261265 [CrossRef] [PubMed].
    [Google Scholar]
  12. Harth E., Romero J., Torres R., Espejo R.T. ( 2007;). Intragenomic heterogeneity and intergenomic recombination among Vibrio parahaemolyticus 16S rRNA genes. Microbiology 153 26402647 [CrossRef] [PubMed].
    [Google Scholar]
  13. Hoffmann M., Monday S.R., Allard M.W., Strain E.A., Whittaker P., Naum M., McCarthy P.J., Lopez J.V., Fischer M., Brown E.W. ( 2012;). Vibrio caribbeanicus sp. nov., isolated from the marine sponge Scleritoderma cyanea . Int J Syst Evol Microbiol 62 17361743 [CrossRef] [PubMed].
    [Google Scholar]
  14. Huss V.A., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kimura M. ( 1983). The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef].
    [Google Scholar]
  16. Kirchberger P.C., Turnsek M., Hunt D.E., Haley B.J., Colwell R.R., Polz M.F., Tarr C.L., Boucher Y. ( 2014;). Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens. Int J Syst Evol Microbiol 64 32083214 [CrossRef] [PubMed].
    [Google Scholar]
  17. Lagesen K., Hallin P., Rødland E.A., Staerfeldt H.H., Rognes T., Ussery D.W. ( 2007;). RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 35 31003108 [CrossRef] [PubMed].
    [Google Scholar]
  18. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., other authors. ( 2007;). clustal w clustal_x version 2.0. Bioinformatics 23 29472948 [CrossRef] [PubMed].
    [Google Scholar]
  19. Lasa A., Diéguez A.L., Romalde J.L. ( 2013;). Vibrio toranzoniae sp. nov., a new member of the Splendidus clade in the genus Vibrio . Syst Appl Microbiol 36 96100 [CrossRef] [PubMed].
    [Google Scholar]
  20. Lasa A., Diéguez A.L., Romalde J.L. ( 2014;). Vibrio cortegadensis sp. nov., isolated from clams. Antonie van Leeuwenhoek 105 335341 [CrossRef] [PubMed].
    [Google Scholar]
  21. Lee J.V., Donovan T.J., Furniss A.L. ( 1978;). Characterization, taxonomy, and emended description of Vibrio metschnikovii . Int J Syst Bacteriol 28 99111 [CrossRef].
    [Google Scholar]
  22. López-López A., Benlloch S., Bonfá M., Rodríguez-Valera F., Mira A. ( 2007;). Intragenomic 16S rDNA divergence in Haloarcula marismortui is an adaptation to different temperatures. J Mol Evol 65 687696 [CrossRef] [PubMed].
    [Google Scholar]
  23. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [CrossRef] [PubMed].
    [Google Scholar]
  24. Nokhal T.H., Schlegel H.G. ( 1983;). Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol 33 2637 [CrossRef].
    [Google Scholar]
  25. Prado S., Dubert J., Romalde J.L., Toranzo A.E., Barja J.L. ( 2014;). Vibrio ostreicida sp. nov., a new pathogen of bivalve larvae. Int J Syst Evol Microbiol 64 16411646 [CrossRef] [PubMed].
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  27. Sawabe T., Kita-Tsukamoto K., Thompson F.L. ( 2007;). Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189 79327936 [CrossRef] [PubMed].
    [Google Scholar]
  28. Sawabe T., Ogura Y., Matsumura Y., Feng G., Amin A.R., Mino S., Nakagawa S., Sawabe T., Kumar R., other authors. ( 2013;). Updating the Vibrio clades defined by multilocus sequence phylogeny: proposal of eight new clades, and the description of Vibrio tritonius sp. nov. Front Microbiol 4 414 [PubMed].
    [Google Scholar]
  29. Sun D.L., Jiang X., Wu Q.L., Zhou N.Y. ( 2013;). Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity. Appl Environ Microbiol 79 59625969 [CrossRef] [PubMed].
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed].
    [Google Scholar]
  31. Thompson F.L., Iida T., Swings J. ( 2004;). Biodiversity of vibrios. Microbiol Mol Biol Rev 68 403431 [CrossRef] [PubMed].
    [Google Scholar]
  32. Thompson F.L., Gevers D., Thompson C.C., Dawyndt P., Naser S., Hoste B., Munn C.B., Swings J. ( 2005;). Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl Environ Microbiol 71 51075115 [CrossRef] [PubMed].
    [Google Scholar]
  33. Thompson F.L., Gomez-Gil B., Vasconcelos A.T.R., Sawabe T. ( 2007;). Multilocus sequence analysis reveals that Vibrio harveyi V. campbellii are distinct species. Appl Environ Microbiol 73 42794285 [CrossRef] [PubMed].
    [Google Scholar]
  34. Tison D.L., Seidler R.J. ( 1983;). Vibrio aestuarianus: a new species from estuarine waters and shellfish. Int J Syst Bacteriol 33 699702 [CrossRef].
    [Google Scholar]
  35. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697703 [PubMed].
    [Google Scholar]
  36. Wilkinson S.G. ( 1988;). Gram-negative bacteria. . In Microbial Lipids vol. 1, pp. 299488. Edited by Ratledge C, Wilkinson S. G. New York: Academic Press;.
    [Google Scholar]
  37. Xu X.W., Wu Y.H., Wang C.S., Oren A., Wu M. ( 2009;). Vibrio hangzhouensis sp. nov., isolated from sediment of the East China Sea. Int J Syst Evol Microbiol 59 20992103 [CrossRef] [PubMed].
    [Google Scholar]
  38. Yoshizawa S., Wada M., Yokota A., Kogure K. ( 2010;). Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water. J Gen Appl Microbiol 56 499507 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000316
Loading
/content/journal/ijsem/10.1099/ijs.0.000316
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error