1887

Abstract

Strain M-SA3-94, an aerobic, Gram-stain-positive, ovoid- to rod-shaped, non-motile bacterium, was isolated from the marine sediment of Ardley cove, King George Island, Antarctica. Strain M-SA3-94 grew optimally at pH 5.0–6.0, 20 °C and in the presence of 1.0 % (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain M-SA3-94 belonged to the genus in the family , clustering with NCIMB 12834, BX5-10, CL-GY44 and D94-1 (with 96.1 %, 95.9 %, 94.5 % and 94.7 % 16S rRNA gene sequence similarity, respectively). The chemotaxonomic properties of strain M-SA3-94 were similar to those of members of the genus with validly published names. The major fatty acid was iso-C. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and three unknown phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan was -2, 6-diaminopimelic acid. MK-8(H4) was the predominant menaquinone and the DNA G+C content of this strain was 66.7 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain M-SA3-94 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-SA3-94 ( = CCTCC AB2014053 = LMG 28254).

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2015-08-01
2019-10-20
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References

  1. Bauer A.W. , Kirby W.M.M. , Sherris J.C. , Turck M. . ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496 [PubMed].
    [Google Scholar]
  2. Bowman J.P. . ( 2000;). ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50: 1861–1868 [PubMed].[CrossRef]
    [Google Scholar]
  3. Busse H.-J. , Schumann P. . ( 1999;). Polyamine profiles within genera of the class Actinobacteria with -diaminopimelic acid in the peptidoglycan. Int J Syst Bacteriol 49: 179–184 [CrossRef] [PubMed].
    [Google Scholar]
  4. Cappuccino J.G. , Sherman N. . ( 2002;). Microbiology: a Laboratory Manual , 6th edn. Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  5. Cho Y. , Jang G.I. , Cho B.C. . ( 2013;). Nocardioides marinquilinus sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 63: 2594–2599 [CrossRef] [PubMed].
    [Google Scholar]
  6. Chou J.H. , Cho N.T. , Arun A.B. , Young C.C. , Chen W.M. . ( 2008;). Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water. Int J Syst Evol Microbiol 58: 1864–1868 [CrossRef] [PubMed].
    [Google Scholar]
  7. Collins M.D. , Keddie R.M. , Kroppenstedt R.M. . ( 1983;). Lipid composition of Arthrobacter simplex, Arthrobacter tumescens and possibly related taxa. Syst Appl Microbiol 4: 18–26 [CrossRef] [PubMed].
    [Google Scholar]
  8. Collins M.D. , Cockcroft S. , Wallbanks S. . ( 1994;). Phylogenetic analysis of a new LL-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44: 523–526 [CrossRef] [PubMed].
    [Google Scholar]
  9. Dastager S.G. , Lee J.C. , Ju Y.J. , Park D.J. , Kim C.J. . ( 2008;). Nocardioides koreensis sp. nov., Nocardioides bigeumensis sp. nov. and Nocardioides agariphilus sp. nov., isolated from soil from Bigeum Island, Korea. Int J Syst Evol Microbiol 58: 2292–2296 [CrossRef] [PubMed].
    [Google Scholar]
  10. Dastager S.G. , Lee J.C. , Ju Y.J. , Park D.J. , Kim C.J. . ( 2009;). Nocardioides sediminis sp. nov., isolated from a sediment sample. Int J Syst Evol Microbiol 59: 280–284 [CrossRef] [PubMed].
    [Google Scholar]
  11. Doetsch R.N. . ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P , Murray R. G. E , Costilow R. N , Nester E. W , Wood W. A , Krieg N. R , Phillips G. H . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  12. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  13. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  14. Holt J.G. , Krieg N.R. , Sneath P.H.A. , Staley J.T. , Williams S.T. . ( 1994;). Nocardioform actinomycetes. . In Shorter Bergey's Manual of Determinative Bacteriology, pp. 625–650. Edited by Hensyl W. R . Baltimore: Williams & Wilkins;.
    [Google Scholar]
  15. Hopwood D.A. , Bibb M.J. , Chater K.F. , Kieser T. , Bruton C.J. , Kieser H.M. , Lydiate D.J. , Smith C.P. , Ward J.M. , Schrempf H. , (editors) . ( 1985;). Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;.
    [Google Scholar]
  16. Kim O.S. , Cho Y.J. , Lee K. , Yoon S.H. , Kim M. , Na H. , Park S.C. , Jeon Y.S. , Lee J.H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  17. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–207 [CrossRef].
    [Google Scholar]
  18. Mesbah M. , Whitman W.B. . ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479: 297–306 [CrossRef] [PubMed].
    [Google Scholar]
  19. Minnikin D. , O'Donnell A. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  20. Prauser H. . ( 1976;). Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26: 58–65 [CrossRef].
    [Google Scholar]
  21. Rzhetsky A. , Nei M. . ( 1992;). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9: 945–967.
    [Google Scholar]
  22. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  23. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  24. Schippers A. , Schumann P. , Spröer C. . ( 2005;). Nocardioides oleivorans sp. nov., a novel crude-oil-degrading bacterium. Int J Syst Evol Microbiol 55: 1501–1504 [CrossRef] [PubMed].
    [Google Scholar]
  25. Song G.C. , Yasir M. , Bibi F. , Chung E.J. , Jeon C.O. , Chung Y.R. . ( 2011;). Nocardioides caricicola sp. nov., an endophytic bacterium isolated from a halophyte, Carex scabrifolia Steud. Int J Syst Evol Microbiol 61: 105–109 [CrossRef] [PubMed].
    [Google Scholar]
  26. Stackebrandt E. , Rainey F.A. , Ward-Rainey N.L. . ( 1997;). Proposal for a new hierarchic classification system, Actinobacteria classic nov. Int J Syst Bacteriol 47: 479–491 [CrossRef].
    [Google Scholar]
  27. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  28. Thompson J.D. , Gibson T.J. , Plewniak F. , Jeanmougin F. , Higgins D.G. . ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  29. Tindall B.J. . ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  30. Tóth E.M. , Kéki Z. , Homonnay Z.G. , Borsodi A.K. , Márialigeti K. , Schumann P. . ( 2008;). Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). Int J Syst Evol Microbiol 58: 78–83 [CrossRef] [PubMed].
    [Google Scholar]
  31. Xie C.H. , Yokota A. . ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49: 345–349 [CrossRef] [PubMed].
    [Google Scholar]
  32. Yoon J.H. , Cho Y.G. , Lee S.T. , Suzuki K. , Nakase T. , Park Y.H. . ( 1999;). Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49: 675–680 [CrossRef] [PubMed].
    [Google Scholar]
  33. Yoon J.H. , Kim I.G. , Lee M.H. , Lee C.H. , Oh T.K. . ( 2005;). Nocardioides alkalitolerans sp nov., isolated from an alkaline serpentinite soil in Korea. Int J Syst Evol Micr 55: 809–814.[CrossRef]
    [Google Scholar]
  34. Zhang D.C. , Schumann P. , Redzic M. , Zhou Y.G. , Liu H.C. , Schinner F. , Margesin R. . ( 2012a;). Nocardioides alpinus sp. nov., a psychrophilic actinomycete isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 62: 445–450 [CrossRef] [PubMed].
    [Google Scholar]
  35. Zhang J. , Ma Y. , Yu H. . ( 2012b;). Nocardioides lianchengensis sp. nov., an actinomycete isolated from soil. Int J Syst Evol Microbiol 62: 2698–2702 [CrossRef] [PubMed].
    [Google Scholar]
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