1887

Abstract

Two Gram-staining-negative, facultatively anaerobic bacterial strains, SG-1 and SG-2, were isolated from a saline soil sample and a compost sample, respectively. The cells were non-motile rods that occurred singly or in chains, and endospores were not observed under tested growth conditions. Optimum growth occurred at 50 °C, pH 7.5–8.0 and with 5–7 % (w/v) NaCl. The DNA G+C content was 49.5–50.5 mol%. The strains contained MK-7 as the predominant menaquinone and iso-C and anteiso-C as the major fatty acids. The polar lipids consisted mainly of diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan type was A1γ (-DAP direct). Phylogenetic analyses revealed that the new isolates belonged to the family , exhibiting low 16S rRNA gene sequence similarity (90.8–91.3 %) to the nearest type strain, YM11-542, and formed a well-supported lineage that was clearly distinguished from all currently described genera in this family. Based on our polyphasic taxonomic characterization, we propose that strains SG-1 and SG-2 represent a novel genus and species within the family , for which we propose the name gen. nov., sp. nov. The type strain of is SG-1 ( = KCTC 33118 = CGMCC 1.12771).

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2015-08-01
2024-12-06
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References

  1. Baker G.C., Smith J.J., Cowan D.A. ( 2003;). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55 541555 [View Article] [PubMed] .
    [Google Scholar]
  2. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed] .
    [Google Scholar]
  3. Combet-Blanc Y., Ollivier B., Streicher C., Patel B.K., Dwivedi P.P., Pot B., Prensier G., Garcia J.L. ( 1995;). Bacillus thermoamylovorans sp. nov., a moderately thermophilic and amylolytic bacterium. Int J Syst Bacteriol 45 916 [View Article] [PubMed] .
    [Google Scholar]
  4. Dong X., Cai M. ( 2001). Manual of Systematic and Determinative Bacteriology Beijing: Academic Press;.
    [Google Scholar]
  5. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  6. Groth I., Schumann P., Rainey F.A., Martin K., Schuetze B., Augsten K. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47 11291133 [View Article] [PubMed] .
    [Google Scholar]
  7. Khelifi N., Ben Romdhane E., Hedi A., Postec A., Fardeau M.L., Hamdi M., Tholozan J.L., Ollivier B., Hirschler-Réa A. ( 2010;). Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia. Extremophiles 14 297304 [View Article] [PubMed] .
    [Google Scholar]
  8. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  9. Logan N.A., Berge O., Bishop A.H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 21142121 [View Article] [PubMed] .
    [Google Scholar]
  10. Márquez M.C., Carrasco I.J., Xue Y., Ma Y., Cowan D.A., Jones B.E., Grant W.D., Ventosa A. ( 2008;). Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. Int J Syst Evol Microbiol 58 19221926 [View Article] [PubMed] .
    [Google Scholar]
  11. Matsuo Y., Katsuta A., Matsuda S., Shizuri Y., Yokota A., Kasai H. ( 2006;). `Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov.. Int J Syst Evol Microbiol 56 28372842 [View Article] [PubMed] .
    [Google Scholar]
  12. Mayr R., Busse H.-J., Worliczek H.L., Ehling-Schulz M., Scherer S. ( 2006;). Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov.. Int J Syst Evol Microbiol 56 13831389 [View Article] [PubMed] .
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  14. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrate procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  15. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI;.
    [Google Scholar]
  16. Schleifer K.H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  17. Schumann P. ( 2011;). Peptidoglycan structure. Methods Microbiol 38 101129 [View Article].
    [Google Scholar]
  18. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P, Murray R. G. E, Wood W. A, Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed] .
    [Google Scholar]
  20. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed] .
    [Google Scholar]
  21. Tiago I., Pires C., Mendes V., Morais P.V., da Costa M.S., Veríssimo A. ( 2006;). Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater. Int J Syst Evol Microbiol 56 25712574 [View Article] [PubMed] .
    [Google Scholar]
  22. Tsubouchi T., Shimane Y., Mori K., Usui K., Hiraki T., Tame A., Uematsu K., Maruyama T., Hatada Y. ( 2013;). Polycladomyces abyssicola gen. nov., sp. nov., a thermophilic filamentous bacterium isolated from hemipelagic sediment. Int J Syst Evol Microbiol 63 19721981 [View Article] [PubMed] .
    [Google Scholar]
  23. Yu T.T., Zhang B.H., Yao J.C., Tang S.K., Zhou E.M., Yin Y.R., Wei D.Q., Ming H., Li W.J. ( 2012;). Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 102 711718 [View Article] [PubMed] .
    [Google Scholar]
  24. Yumoto I., Yamazaki K., Sawabe T., Nakano K., Kawasaki K., Ezura Y., Shinano H. ( 1998;). Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus. Int J Syst Bacteriol 48 565571 [View Article] [PubMed] .
    [Google Scholar]
  25. Zachara J.M., Fredrickson J.K., Li S.M., Kennedy D.W., Smith S.C., Gassman P.L. ( 1998;). Bacterial reduction of crystalline Fe3+ oxides in single phase suspensions and subsurface materials. Am Mineral 83 14261443.
    [Google Scholar]
  26. Zhang H., Sekiguchi Y., Hanada S., Hugenholtz P., Kim H., Kamagata Y., Nakamura K. ( 2003;). Gemmatimonas aurantiaca gen. nov., sp. nov., a Gram-negative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum Gemmatimonadetes phyl. nov.. Int J Syst Evol Microbiol 53 11551163 [View Article] [PubMed] .
    [Google Scholar]
  27. Zhang L., Wang Y., Dai J., Tang Y., Yang Q., Luo X., Fang C. ( 2009;). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. Int J Syst Evol Microbiol 59 17871792 [View Article] [PubMed] .
    [Google Scholar]
  28. Zhao W., Zhang C.L., Romanek C.S., Wiegel J. ( 2008;). Description of Caldalkalibacillus uzonensis sp. nov. and emended description of the genus Caldalkalibacillus . Int J Syst Evol Microbiol 58 11061108 [View Article] [PubMed] .
    [Google Scholar]
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