1887

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, short rod-shaped and moderately halophilic bacterial strain, CAU 1105, was isolated from soil on reclaimed tidal land in Modo, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1105 grows optimally at a temperature of 37 °C at pH 7 in the presence of 3 % (w/v) sea salt. Based on 16S rRNA gene sequence similarity analyses, the novel isolate was assigned to the genus within the class and showed the highest 16S rRNA gene sequence similarity with B33 (95.4 %). Strain CAU 1105 contained ubiquinone-10 as the only respiratory quinone and Cω7 as the major cellular fatty acid. The DNA G+C content of strain CAU 1105 was 64.3 mol%. On the basis of phenotypic differentiation, phylogenetic and chemotaxonomic data, strain CAU 1105 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 1105 ( = KCTC 42298 = NBRC 110740). An emended description of the genus is also provided.

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2015-08-01
2020-09-20
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References

  1. Benson D.A., Cavanaugh M., Clark K., Karsch-Mizrachi I., Lipman D.J., Ostell J., Sayers E.W. ( 2013;). GenBank. Nucleic Acids Res 41 (D1), D36D42 [CrossRef] [PubMed].
    [Google Scholar]
  2. Buchan A., González J.M., Moran M.A. ( 2005;). Overview of the marine roseobacter lineage. Appl Environ Microbiol 71 56655677 [CrossRef] [PubMed].
    [Google Scholar]
  3. Cappuccino J.G., Sherman N. ( 2002). Microbiology: A Laboratory Manual , 6th edn. Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  4. Choi D.H., Cho J.-C., Lanoil B.D., Giovannoni S.J., Cho B.C. ( 2007;). Maribius salinus gen. nov., sp. nov., isolated from a solar saltern and Maribius pelagius sp. nov., cultured from the Sargasso Sea, belonging to the Roseobacter clade. Int J Syst Evol Microbiol 57 270275 [CrossRef] [PubMed].
    [Google Scholar]
  5. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  7. Felsenstein J. ( 1989;). phylip – phylogeny inference package (version 3.2). Cladistics 5 164166.
    [Google Scholar]
  8. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [CrossRef].
    [Google Scholar]
  9. Fitch W.M., Margoliash E. ( 1967;). Construction of phylogenetic trees. Science 155 279284 [CrossRef] [PubMed].
    [Google Scholar]
  10. Gordon R.E., Mihm J.M. ( 1962;). Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98 628636 [CrossRef].
    [Google Scholar]
  11. Jukes T.H., Cantor C.R. ( 1969). [CrossRef] Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. H. New York: Academic Press;.
    [Google Scholar]
  12. Kim J.M., Le N.T., Chung B.S., Park J.H., Bae J.-W., Madsen E.L., Jeon C.O. ( 2008;). Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o., m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 74 73137320 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [CrossRef].
    [Google Scholar]
  15. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  16. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 167 [CrossRef].
    [Google Scholar]
  17. Larkin M.A., Blackshields G,, Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., other authors. ( 2007;). clustal w and clustal x version 2.0. Bioinformatics 23 29472948 [CrossRef] [PubMed].
    [Google Scholar]
  18. Lee K., Choo Y.-J., Giovannoni S.J., Cho J.-C. ( 2007;). Maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 57 16531658 [CrossRef] [PubMed].
    [Google Scholar]
  19. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [CrossRef].
    [Google Scholar]
  20. Martínez-Checa F., Quesada E., Martínez-Cánovas M.J., Llamas I., Béjar V. ( 2005;). Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the ‘Alphaproteobacteria’, isolated from a saline soil. Int J Syst Evol Microbiol 55 25252530 [CrossRef] [PubMed].
    [Google Scholar]
  21. Minnikin D.E., Hutchinson I.G., Caldicott A.B., Goodfellow M. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188 221233 [CrossRef].
    [Google Scholar]
  22. Moraine R.A., Rogovin P. ( 1966;). Kinetics of polysaccharide B-1459 fermentation. Biotechnol Bioeng 8 511524 [CrossRef].
    [Google Scholar]
  23. Parte A.C. ( 2014;). LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42 (D1), D613D616 [CrossRef] [PubMed].
    [Google Scholar]
  24. Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1981;). Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microb Ecol 7 235243 [CrossRef] [PubMed].
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  26. Shiba T. ( 1991;). Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a . Syst Appl Microbiol 14 140145 [CrossRef].
    [Google Scholar]
  27. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  28. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [CrossRef].
    [Google Scholar]
  29. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., Vandamme P. ( 2009;). Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 59 811817 [CrossRef] [PubMed].
    [Google Scholar]
  30. Wang Y.-X., Wang Z.-G., Liu J.-H., Chen Y.-G., Zhang X.-X., Wen M.-L., Xu L.-H., Peng Q., Cui X.-L. ( 2009;). Sediminimonas qiaohouensis gen. nov., sp. nov., a member of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 59 15611567 [CrossRef] [PubMed].
    [Google Scholar]
  31. Yoon J.-H., Kim I.-G., Lee M.-H., Lee C.-H., Oh T.-K. ( 2005;). Nocardioides alkalitolerans sp. nov., isolated from an alkaline serpentinite soil in Korea. Int J Syst Evol Microbiol 55 809814 [CrossRef] [PubMed].
    [Google Scholar]
  32. Yoon J.-H., Kang S.J., Lee J.S., Oh T.K. ( 2009;). Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 59 4852 [CrossRef] [PubMed].
    [Google Scholar]
  33. Zan J., Liu Y., Fuqua C., Hill R.T. ( 2014;). Acyl-homoserine lactone quorum sensing in the Roseobacter clade. Int J Mol Sci 15 654669 [CrossRef] [PubMed].
    [Google Scholar]
  34. Zheng Q., Chen C., Yan X.-J., Wang Y.-N., Zeng Y.-H., Hao L.-K., He W.-H., Jiao N.-Z. ( 2010;). Mameliella alba gen. nov., sp. nov., a marine bacterium of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 60 953957 [CrossRef] [PubMed].
    [Google Scholar]
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