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Abstract

A bacterial strain, designated KUFDS01, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20 % bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus . Strain KUFDS01 was closely related to JCM 13863 (96.6 % sequence similarity). An gene sequence analysis indicated that strain KUFDS01 was different from JCM 13863, with a sequence similarity value of 87.8 %. The major cellular fatty acids of strain KUFDS01 were C, iso-C, anteiso-C, Cω6, 9 and Cω9. The DNA G+C content of strain KUFDS01 was 57.7 ± 0.66 mol%. On the basis of these data, strain KUFDS01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is KUFDS01 ( = JCM 30531 = CCUG 66735).

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2015-08-01
2020-09-25
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References

  1. Dewhirst F.E., Chen T., Izard J., Paster B.J., Tanner A.C.R., Yu W.-H., Lakshmanan A., Wade W.G. ( 2010;). The human oral microbiome. J Bacteriol 192 50025017 [CrossRef] [PubMed].
    [Google Scholar]
  2. Felsenstein J. ( 1985;). Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  3. Holdeman L.V., Cato E.P., Moore W.E.C. ( 1977). Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  4. Kazor C.E., Mitchell P.M., Lee A.M., Stokes L.N., Loesche W.J., Dewhirst F.E., Paster B.J. ( 2003;). Diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients. J Clin Microbiol 41 558563 [CrossRef] [PubMed].
    [Google Scholar]
  5. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [CrossRef] [PubMed].
    [Google Scholar]
  6. Kuykendall L.D., Roy M.A., O'Neill J.J., Devine T.E. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38 358361 [CrossRef].
    [Google Scholar]
  7. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., other authors. ( 2007;). clustal w clustal_x version 2.0. Bioinformatics 23 29472948 [CrossRef] [PubMed].
    [Google Scholar]
  8. McClung L.S., Lindberg R.B. ( 1957;). The study of obligately anaerobic bacteria. . In Mannual of Microbiological Methods, pp. 120139. Edited by Pelczar M. J., other authors. New York: McGraw-Hill;.
    [Google Scholar]
  9. Miller L.T. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16 584586 [PubMed].
    [Google Scholar]
  10. Moore L.V.H., Moore W.E.C. ( 1994;). Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic gram-negative bacilli from the human gingival crevice. Int J Syst Bacteriol 44 187192 [CrossRef] [PubMed].
    [Google Scholar]
  11. Paster B.J., Boches S.K., Galvin J.L., Ericson R.E., Lau C.N., Levanos V.A., Sahasrabudhe A., Dewhirst F.E. ( 2001;). Bacterial diversity in human subgingival plaque. J Bacteriol 183 37703783 [CrossRef] [PubMed].
    [Google Scholar]
  12. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  13. Sakamoto M. ( 2014;). The family Porphyromonadaceae . . In The Prokaryotes , 4th edn., pp. 811824. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. Berlin: Springer; [CrossRef].
    [Google Scholar]
  14. Sakamoto M., Ohkuma M. ( 2010;). Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods. J Med Microbiol 59 12931302 [CrossRef] [PubMed].
    [Google Scholar]
  15. Sakamoto M., Ohkuma M. ( 2011;). Identification and classification of the genus Bacteroides by multilocus sequence analysis. Microbiology 157 33883397 [CrossRef] [PubMed].
    [Google Scholar]
  16. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002;). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 52 841849 [CrossRef] [PubMed].
    [Google Scholar]
  17. Sakamoto M., Huang Y., Umeda M., Ishikawa I., Benno Y. ( 2005;). Prevotella multiformis sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55 815819 [CrossRef] [PubMed].
    [Google Scholar]
  18. Sakamoto M., Suzuki N., Benno Y. ( 2010;). hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes . Int J Syst Evol Microbiol 60 29842990 [CrossRef] [PubMed].
    [Google Scholar]
  19. Shah H.N. ( 1992;). The genus Bacteroides and related taxa. . In The Prokaryotes , 2nd edn., pp. 35933607. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef].
    [Google Scholar]
  20. Takeshita T., Nakano Y., Kumagai T., Yasui M., Kamio N., Shibata Y., Shiota S., Yamashita Y. ( 2009;). The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status. ISME J 3 6578 [CrossRef] [PubMed].
    [Google Scholar]
  21. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [CrossRef].
    [Google Scholar]
  22. Willems A., Collins M.D. ( 1995;). Reclassification of Oribaculum catoniae (Moore and Moore 1994) as Porphyromonas catoniae comb. nov. and emendation of the genus Porphyromonas . Int J Syst Bacteriol 45 578581 [CrossRef] [PubMed].
    [Google Scholar]
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