1887

Abstract

A hyperthermophilic crenarchaeon, designated strain CBA1502, was isolated from volcanic soil in the Mayon volcano in the Philippines. The 16S rRNA gene sequence of strain CBA1502 was most closely related to that of DSM 5263 (99.2 % similarity) and Kra 1 (99.0 %). The organism grew at 75–90 °C and pH 4.0–6.0 and in the presence of 0–0.5 % (w/v) NaCl, with optimal growth at 85 °C and pH 5.0. Strain CBA1502 utilized -arabinose, beef extract, Casamino acids, formate, fumarate, peptone, pyruvate, trimethylamine and yeast extract as energy substrates, and -arabinose, formate, pyruvate and yeast extract as carbon sources. Fumarate, sulfate, sulfur and thiosulfate functioned as electron acceptors, but not ferric chloride, nitrate, malate or oxidized glutathione. DNA–DNA hybridization studies showed that there was less than 46.1 % relatedness between strain CBA1502 and other members of the genus . The DNA G+C content of strain CBA1502 was 62.0 mol%. We conclude that, according to its phylogenetic, phenotypic and genotypic characteristics, strain CBA1502 represents a novel species of the genus , and propose the name sp. nov., with the type strain CBA1502 ( = ATCC BAA-2416 = JCM 17229).

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2015-08-01
2024-03-28
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References

  1. Bonch-Osmolovskaya E., Miroshnichenko M., Kostrikina N., Chernych N., Zavarzin G. ( 1990;). Thermoproteus uzoniensis sp. nov., a new extremely thermophilic archaebacterium from Kamchatka continental hot springs. Arch Microbiol 154 556559. [CrossRef]
    [Google Scholar]
  2. Burggraf S., Huber H., Stetter K.O. ( 1997;). Reclassification of the crenarchael orders and families in accordance with 16S rRNA sequence data. Int J Syst Bacteriol 47 657660 [View Article] [PubMed].
    [Google Scholar]
  3. Chan P.P., Cozen A.E., Lowe T.M. ( 2013;). Reclassification of Thermoproteus neutrophilus Stetter and Zillig 1989 as Pyrobaculum neutrophilum comb. nov. based on phylogenetic analysis. Int J Syst Evol Microbiol 63 751754 [View Article] [PubMed].
    [Google Scholar]
  4. Cui H.L., Lin Z.Y., Dong Y., Zhou P.J., Liu S.J. ( 2007;). Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57 22042206 [View Article] [PubMed].
    [Google Scholar]
  5. Ezaki T., Hashimoto H., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  6. Garrity G.M., Holt J.G. ( 2001;). Phylum AI. Crenarchaeota phy. nov. . In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, The Archaea and the Deeply Branching and Phototrophic Bacteria, p. 169. Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer; [View Article].
    [Google Scholar]
  7. González J.M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773 [View Article] [PubMed].
    [Google Scholar]
  8. Lee H.W., Roh S.W., Shin N.R., Lee J., Whon T.W., Jung M.J., Yun J.H., Kim M.S., Hyun D.W., other authors. ( 2013;). Blastopirellula cremea sp. nov., isolated from a dead ark clam. Int J Syst Evol Microbiol 63 23142319 [View Article] [PubMed].
    [Google Scholar]
  9. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  10. Stetter K.O., Zillig W. ( 1989;). Genus I. Thermoproteus Zillig and Stetter 1982, 267VP (effective publication Zillig and Stetter in Zillig, Stetter, Shäfer, Janekovic, Wunderl, Holz and Palm 1981, 225). . In Bergey's Manual of Systematic Bacteriology, p. 2241. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  11. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology, Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  12. Yim K.J., Cha I.T., Rhee J.K., Song H.S., Hyun D.W., Lee H.W., Kim D., Kim K.N., Nam Y.D., other authors. ( 2015;). Vulcanisaeta thermophila sp. nov., a hyperthermophilic and acidophilic crenarchaeon isolated from solfataric soil. Int J Syst Evol Microbiol 65 201205 [View Article] [PubMed].
    [Google Scholar]
  13. Zillig W., Stetter K.O., Schäfer W., Janekovic D., Wunderl S., Holz I., Palm P. ( 1981;). Thermoproteales: a novel type of extremely thermoacidophilic anaerobic archaebacteria isolated from Icelandic solfataras. Zentralbl Bakteriol Mikrobiol Hyg 1 Abt Orig C 2 205227.
    [Google Scholar]
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