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Abstract

A hyperthermophilic crenarchaeon, designated strain CBA1502, was isolated from volcanic soil in the Mayon volcano in the Philippines. The 16S rRNA gene sequence of strain CBA1502 was most closely related to that of DSM 5263 (99.2 % similarity) and Kra 1 (99.0 %). The organism grew at 75–90 °C and pH 4.0–6.0 and in the presence of 0–0.5 % (w/v) NaCl, with optimal growth at 85 °C and pH 5.0. Strain CBA1502 utilized -arabinose, beef extract, Casamino acids, formate, fumarate, peptone, pyruvate, trimethylamine and yeast extract as energy substrates, and -arabinose, formate, pyruvate and yeast extract as carbon sources. Fumarate, sulfate, sulfur and thiosulfate functioned as electron acceptors, but not ferric chloride, nitrate, malate or oxidized glutathione. DNA–DNA hybridization studies showed that there was less than 46.1 % relatedness between strain CBA1502 and other members of the genus . The DNA G+C content of strain CBA1502 was 62.0 mol%. We conclude that, according to its phylogenetic, phenotypic and genotypic characteristics, strain CBA1502 represents a novel species of the genus , and propose the name sp. nov., with the type strain CBA1502 ( = ATCC BAA-2416 = JCM 17229).

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2015-08-01
2019-10-21
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