1887

Abstract

A novel bacterial strain, designated JBR12, was isolated from sediment of a shallow stream in Cheonan, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JBR12 belongs to the genus , and indicated that its closest relatives are DSM 17448 (97.8  % sequence similarity) and Gsoil 085 (94.3  %). A DNA–DNA hybridization experiment revealed < 70  % genomic relatedness between strain JBR12 and DSM 17448. The major fatty acids (>5  % of the total) were iso-C, summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH, anteiso-C and iso-C 3-OH. The DNA G+C content of strain JBR12 was 37.7 mol%. According to data from the present polyphasic taxonomic study, strain JBR12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBR12 ( = KACC 17466 = JCM 19321).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000292
2015-08-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2496.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000292&mimeType=html&fmt=ahah

References

  1. Álvarez B., Secades P., Prieto M., McBride M.J., Guijarro J.A.. ( 2006;). A mutation in Flavobacterium psychrophilum tlpB inhibits gliding motility and induces biofilm formation. Appl Environ Microbiol 72: 4044–4053 [CrossRef] [PubMed].
    [Google Scholar]
  2. Baik K.S., Park Y.D., Seong C.N., Kim E.M., Bae K.S., Chun J.. ( 2006;). Glaciecola nitratireducens sp. nov., isolated from seawater. Int J Syst Evol Microbiol 56: 2185–2188 [CrossRef] [PubMed].
    [Google Scholar]
  3. Bernardet J.F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [CrossRef] [PubMed].
    [Google Scholar]
  4. Hall T.A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
    [Google Scholar]
  5. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  6. Kim W.H., Lee S., Ahn T.Y.. ( 2014;). Flavihumibacter cheonanensis sp. nov., isolated from sediment of a shallow stream. Int J Syst Evol Microbiol 64: 3235–3239 [CrossRef] [PubMed].
    [Google Scholar]
  7. Lee S.W., Oh H.W., Lee K.H., Ahn T.Y.. ( 2009;). Methylobacterium dankookense sp. nov., isolated from drinking water. J Microbiol 47: 716–720 [CrossRef] [PubMed].
    [Google Scholar]
  8. Lee S., Weon H.Y., Han K., Ahn T.Y.. ( 2012;). Flavobacterium dankookense sp. nov., isolated from a freshwater reservoir, and emended descriptions of Flavobacterium cheonanense F. chungnamense F. koreense F. aquatile. Int J Syst Evol Microbiol 62: 2378–2382 [CrossRef] [PubMed].
    [Google Scholar]
  9. Liu Q.M., Ten L.N., Yu H.S., Jin F.X., Im W.T., Lee S.T.. ( 2008;). Emticicia ginsengisoli sp. nov., a species of the family ‘Flexibacteraceae’ isolated from soil of a ginseng field. Int J Syst Evol Microbiol 58: 1100–1105 [CrossRef] [PubMed].
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W.B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  11. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  12. Moore D.D., Dowhan D.. ( 1995;). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. New York Wiley:.
    [Google Scholar]
  13. Saha P., Chakrabarti P.. ( 2006;). Emticicia oligotrophica gen. nov., sp. nov., a new member of the family ‘Flexibacteraceae’, phylum Bacteroidetes. Int J Syst Evol Microbiol 56: 991–995 [CrossRef] [PubMed].
    [Google Scholar]
  14. Seldin L., Dubnau D.. ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35: 151–154 [CrossRef].
    [Google Scholar]
  15. Smibert R.M., Krieg N.R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000292
Loading
/content/journal/ijsem/10.1099/ijs.0.000292
Loading

Data & Media loading...

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error