1887

Abstract

sp. nov. (ex-type culture CCTU 1813 = OPU 1626 = CBS 139567) is a novel oomycete species isolated from with snow rot symptoms in a natural grassland in East-Azarbaijan province, Iran. Phylogenetic analyses based on sequence data from internal transcribed spacer (ITS)-rDNA, and mitochondrial genes clustered our isolates in group E as a unique, well supported clade. sp. nov. is phylogenetically and morphologically distinct from the other closely related species in this clade, namely and . sp. nov. can be distinguished from these two species by its cylindrical sporangia and lower temperatures for optimum and maximum growth rate. The development of zoospores released through a shorter discharge tube is an additional morphological feature which can be used to differentiate sp. nov. from . Laboratory inoculation tests demonstrated the pathogenicity of sp. nov. to under wet cold (0–3 °C) conditions.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000291
2015-08-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2500.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000291&mimeType=html&fmt=ahah

References

  1. Ali-Shtayeh M.S. , Len L.C. , Dick M.W. . ( 1986;). An improved method and medium for quantitative estimates of populations of Pythium species from soil. Transactions of the British Mycological Society 86: 39–47 [CrossRef].
    [Google Scholar]
  2. Bakhshi M. , Arzanlou M. , Babai-Ahari A. , Groenewald J.Z. , Braun U. , Crous P.W. . ( 2014;). Multi-gene analysis of Pseudocercospora spp. from Iran. Phytotaxa 184: 245–264 [CrossRef].
    [Google Scholar]
  3. Bakhshi M. , Arzanlou M. , Babai-Ahari A. , Groenewald J.Z. , Braun U. , Crous P.W. . ( 2015;). Application of the consolidated species concept to Cercospora spp. from Iran. Persoonia 34: 65–86.[CrossRef]
    [Google Scholar]
  4. de Cock A.W.A.M. , Lévesque C.A. . ( 2004;). New species of Pythium and Phytophthora. Stud Mycol 50: 481–487.
    [Google Scholar]
  5. Drummond A. , Ashton B. , Buxton S. , Cheung M. , Cooper A. , Duran C. , Field M. , Heled J. , Kearse M. , other authors . ( 2012;). Geneious v 5.6, Available from http://www.geneious.com .
  6. Hirane S. . ( 1960;). [Studies on Pythium snow blight of wheat and barley, with special reference to the taxonomy of the pathogens]. Trans Mycol Soc Jpn 2: 82–87 (in Japanese with English abstract).
    [Google Scholar]
  7. Hong C.X. , Moorman G.W. . ( 2005;). Plant pathogens in irrigation water: challenges and opportunities. Crit Rev Plant Sci 24: 189–208 [CrossRef].
    [Google Scholar]
  8. Ito S. . ( 1935;). Notae mycologicae asiae orientalis I. Transactions of the Sapporo Natural History Society 14: 11–33.
    [Google Scholar]
  9. Kageyama K. . ( 2014;). Molecular taxonomy and its application to ecological studies of Pythium species. J Gen Plant Pathol 80: 314–326 [CrossRef].
    [Google Scholar]
  10. Katoh K. , Toh H. . ( 2008;). Recent developments in the mafft multiple sequence alignment program. Brief Bioinform 9: 286–298 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kawamura Y. , Yokoo K. , Tojo M. , Hishiike M. . ( 2005;). Distribution of Pythium porphyrae, the causal agent of red rot disease of Porphyra spp., in the Ariake Sea, Japan. Plant Dis 89: 1041–1047 [CrossRef].
    [Google Scholar]
  12. Kirk P.M. , Cannon P.F. , Minter D.W. , Stalpers J.A. . ( 2008;). Ainsworth & Bisby's Dictionary of the Fungi , 10th edn.., Wallingford: CAB International;.
    [Google Scholar]
  13. Lévesque C.A. , de Cock A.W.A.M. . ( 2004;). Molecular phylogeny and taxonomy of the genus Pythium. Mycol Res 108: 1363–1383 [CrossRef] [PubMed].
    [Google Scholar]
  14. Lipps P.E. . ( 1980;). A new species of Pythium isolated from wheat beneath snow in Washington. Mycologia 72: 1127–1133 [CrossRef].
    [Google Scholar]
  15. Martin F.N. . ( 1992;). Pythium. . In Methods for Research on Soilborne Phytopathogenic Fungi, pp. 39–49. Edited by Singleton L. L. , Mihail J. D. , Rush C. M. . St. Paul, MN: American Phytopathological Society;.
    [Google Scholar]
  16. Martin F.N. . ( 2000;). Phylogenetic relationships among some Pythium species inferred from sequence analysis of the mitochondrially encoded cytochrome oxidase II gene. Mycologia 92: 711–727 [CrossRef].
    [Google Scholar]
  17. Martin F.N. , Loper J.E. . ( 1999;). Soil-borne plant diseases caused by Pythium spp.: Ecology, epidemiology, and prospects for biological control. Crit Rev Plant Sci 18: 111–181 [CrossRef].
    [Google Scholar]
  18. Miller P.M. . ( 1955;). V-8 juice agar as a general purpose medium for fungi and bacteria. Phytopathology 45: 461–462.
    [Google Scholar]
  19. Miura M. , Hatai K. , Tojo M. , Wada S. , Kobayashi S. , Okazaki T. . ( 2010;). Visceral mycosis in Ayu Plecoglossus altivelis larvae caused by Pythium flevoense. Fish Pathol 45: 24–30 [CrossRef].
    [Google Scholar]
  20. Möller E.M. , Bahnweg G. , Sandermann H. , Geiger H.H. . ( 1992;). A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 20: 6115–6116 [CrossRef] [PubMed].
    [Google Scholar]
  21. Morita Y. , Tojo M. . ( 2007;). Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil. Plant Dis 91: 1591–1599 [CrossRef].
    [Google Scholar]
  22. Mostowfizadeh-Ghalamfarsa R. , Banihashemi Z. . ( 2005;). Identification of soil Pythium species in Fars province of Iran. Iran J Sci Technol Trans A Sci 29: 79–87.
    [Google Scholar]
  23. Nechwatal J. , Wielgoss A. , Mendgen K. . ( 2008;). Diversity, host, and habitat specificity of oomycete communities in declining reed stands (Phragmites australis) of a large freshwater lake. Mycol Res 112: 689–696 [CrossRef] [PubMed].
    [Google Scholar]
  24. Nylander J.A.A. . ( 2004;). MrModeltest v2.0.Program distributed by the author. Sweden: Evolutionary Biology Centre, Uppsala University;.
  25. Petrov V.F. . ( 1983;). [Pathogenic microflora of root growth in perennial grasses in Khibiny]. Bulletin of Applied Botany, Genetics and Plant Breeding 82: 38–45 (in Russian with English abstract).
    [Google Scholar]
  26. Phillips A.J. , Anderson V.L. , Robertson E.J. , Secombes C.J. , van West P. . ( 2008;). New insights into animal pathogenic oomycetes. Trends Microbiol 16: 13–19 [CrossRef] [PubMed].
    [Google Scholar]
  27. Ronquist F. , Huelsenbeck J.P. . ( 2003;). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574 [CrossRef] [PubMed].
    [Google Scholar]
  28. Takamatsu S. , Takenaka S. . ( 2001;). Snow rot caused by Pythium species. . In Low Temperature Plant Microbe Interactions Under Snow, pp. 87–100. Edited by Iriki N. , Gaudet D. A. , Tronsmo A. M. , Matsumoto N. , Yoshida M. , Nishimune A. . Sapporo: Hokkaido National Agricultural Experimental Station;.
    [Google Scholar]
  29. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  30. Tojo M. , Newsham K.K. . ( 2012;). Snow mould in polar environments. Fungal Ecol 5: 395–402 [CrossRef].
    [Google Scholar]
  31. Van der Plaats-Niterink A.J. . ( 1981;). Monograph of the genus Pythium. Studies in Mycology no. 21. Baarn: Centraalbureau voor Schimmelcultures;.
  32. Villa N.O. , Kageyama K. , Asano T. , Suga H. . ( 2006;). Phylogenetic relationships of Pythium and Phytophthora species based on ITS rDNA, cytochrome oxidase II and beta-tubulin gene sequences. Mycologia 98: 410–422 [CrossRef] [PubMed].
    [Google Scholar]
  33. White T.J. , Bruns T. , Lee S. , Taylor J. . ( 1990;). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. [CrossRef] In PCR Protocols, a Guide to Methods and Applications, pp. 315–322. Edited by Innis M. A. , Gelfand D. H. , Sninsky J. J. , White T. J. . San Diego, CA: Academic Press;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000291
Loading
/content/journal/ijsem/10.1099/ijs.0.000291
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error