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Abstract

A Gram-stain-negative, aerobic bacterium, designated strain YIM 77409, was isolated from the Niujie hot spring in the Eryuan county of Dali, Yunnan province, south-west China. Cells of the strain were rod-shaped and colonies were yellow and circular. The strain grew at pH 6.0–8.0 (optimum, pH 7.0) and 50–70°C (optimum, 60–65°C). The predominant menaquinone was MK-8 and the DNA G+C content was 66.4 mol%. Major fatty acids (>10 %) were iso-C and iso-C.The polar lipids consisted of one aminophospholipid, one phospholipid and two glycolipids. 16S rRNA gene sequence analysis showed that strain YIM 77409 formed a cluster with DSM 8553, DSM 12462, YIM 77925 and YIM 77924, with highest 16S rRNA gene sequence similarity to DSM 8553 (97.57 %). However, DNA–DNA hybridization indicated that strain YIM 77409 should be viewed as a representative of a novel species, as there was only 30.6 ± 1.6 % reassociation with DSM 8553. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain YIM 77409 represents a novel species of the genus , with the name sp. nov. The type strain is YIM 77409 ( = DSM 25898 = KCTC 32024).

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2015-08-01
2019-10-14
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