1887

Abstract

An extremely halophilic archaeal strain, halo-7, was isolated from brine sediment of the Gomso solar saltern, Republic of Korea. Cells of strain halo-7 were pleomorphic, stained Gram-negative, lysed in distilled water and formed red-pigmented colonies. Strain halo-7 grew in the range of 25–45 °C (optimum 37–40 °C), pH 6.5–9.5 (optimum pH 7.0–8.0), 15–30 % (w/v) NaCl (optimum, 20–25 %), and 0.05–0.5 M MgCl (optimum 0.1–0.3 M). The minimal NaCl concentration to prevent cell lysis of strain halo-7 was 10 % (w/v). The major polar lipids of the isolate were phosphatidylglycerol, phosphatidylglycerolphosphate methyl ester, an unidentified phospholipid, an unidentified lipid, and two unidentified glycolipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-7 is closely related to the members of the genus , WSY15-H3 (98.7 % 16S rRNA gene sequence similarity), TBN21 (98.2 %) and CBA1107 (97.4 %). The genomic DNA G+C content determined for strain halo-7 (68.0 mol%) was slightly higher than those of JCM 18549 and CBA1107. DNA–DNA hybridization values between strain halo-7 and reference strains were < 25 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe a novel species of the genus , represented by strain halo-7, for which we propose the name sp. nov. The type strain is halo-7 ( = JCM 30187 = CECT 8739).

Funding
This study was supported by the:
  • the project on survey of indigenous species of Korea of the National Institute of Biological Resources (Award 2012R1A1A2040922)
  • Basic Science Research Program
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000287
2015-08-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2479.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000287&mimeType=html&fmt=ahah

References

  1. Atlas R.M. ( 2004). Handbook of Microbiological Media , 3rd edn. Boca Raton, FL: CRC Press; [View Article].
    [Google Scholar]
  2. Benson H.J. ( 2002). Microbiological Applications: Laboratory Manual in General Microbiology New York: McGraw-Hill;.
    [Google Scholar]
  3. Cha I.T., Yim K.J., Song H.S., Lee H.W., Hyun D.W., Kim K.N., Choi J.S., Kim D., Lee S.J., other authors. ( 2014;). Halolamina rubra sp. nov., a haloarchaeon isolated from non-purified solar salt. Antonie van Leeuwenhoek 105 907914 [View Article] [PubMed] .
    [Google Scholar]
  4. Cui H.L., Lin Z.Y., Dong Y., Zhou P.J., Liu S.J. ( 2007;). Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57 22042206 [View Article] [PubMed] .
    [Google Scholar]
  5. Cui H.L., Gao X., Yang X., Xu X.W. ( 2011;). Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae . Int J Syst Evol Microbiol 61 16171621 [View Article] [PubMed].
    [Google Scholar]
  6. DeLong E.F. ( 1992;). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89 56855689 [View Article] [PubMed].
    [Google Scholar]
  7. Dittmer J.C., Lester R.L. ( 1964;). A Simple, Specific Spray for the Detection of Phospholipids on Thin-Layer Chromatograms. J Lipid Res 5 126127 [PubMed].
    [Google Scholar]
  8. Dussault H.P. ( 1955;). An improved technique for staining red halophilic bacteria. J Bacteriol 70 484485 [PubMed].
    [Google Scholar]
  9. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229. [CrossRef]
    [Google Scholar]
  10. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  11. Gonzalez J.M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773 [View Article] [PubMed].
    [Google Scholar]
  12. Gonzalez C., Gutierrez C., Ramirez C. ( 1978;). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24 710715 [View Article] [PubMed].
    [Google Scholar]
  13. Gupta R.S., Naushad S., Baker S. ( 2015;). Phylogenomic analyses and molecular signatures for the class Halobacteria and its two major clades: a proposal for division of the class Halobacteria into an emended order Halobacteriales and two new orders, Haloferacales ord. nov. and Natrialbales ord. nov., containing the novel families Haloferacaceae fam. nov. and Natrialbaceae fam. nov. Int J Syst Evol Microbiol 65 10501069 [View Article] [PubMed].
    [Google Scholar]
  14. Gutiérrez M.C., Castillo A.M., Kamekura M., Ventosa A. ( 2008;). Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake. Int J Syst Evol Microbiol 58 28802884 [View Article] [PubMed].
    [Google Scholar]
  15. Kidd K.K., Sgaramella-Zonta L.A. ( 1971;). Phylogenetic analysis: concepts and methods. Am J Hum Genet 23 235252 [PubMed] .
    [Google Scholar]
  16. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  17. Kimura M. ( 1983). The Neutral Theory of Molecular Evolution Cambridge, UK: Cambridge University Press; [View Article].
    [Google Scholar]
  18. Oren A., Duker S., Ritter S. ( 1996;). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138 135140 [View Article].
    [Google Scholar]
  19. Oren A., Ventosa A., Grant W.D. ( 1997;). Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47 233238 [View Article].
    [Google Scholar]
  20. Park S.J., Cha I.T., Kim S.J., Shin K.S., Hong Y., Roh D.H., Rhee S.K. ( 2012;). Salinisphaera orenii sp. nov., isolated from a solar saltern. Int J Syst Evol Microbiol 62 18771883 [View Article] [PubMed].
    [Google Scholar]
  21. Pruesse E., Peplies J., Glöckner F.O. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28 18231829 [View Article] [PubMed].
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  23. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  24. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  25. Tittsler R.P., Sandholzer L.A. ( 1936;). The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31 575580 [PubMed].
    [Google Scholar]
  26. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697703 [PubMed].
    [Google Scholar]
  27. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. ( 2000;). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50 12971303 [View Article] [PubMed].
    [Google Scholar]
  28. Zhang W.Y., Huo Y.Y., Zhang X.Q., Zhu X.F., Wu M. ( 2013;). Halolamina salifodinae sp. nov. and Halolamina salina sp. nov., two extremely halophilic archaea isolated from a salt mine. Int J Syst Evol Microbiol 63 43804385 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000287
Loading
/content/journal/ijsem/10.1099/ijs.0.000287
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error