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Volume 65, Issue Pt_8

sp. nov., an extremely alkaliphilic bacterium, and emended description of genus Free

Abstract

A novel aerobic, alkaliphilic, Gram-staining-positive, endospore-forming bacterium, strain OMN17, was isolated from a typical sandy loam soil under long-term OMN fertilization (half organic manure N plus half mineral N fertilizer) in northern China and was subjected to a polyphasic taxonomic study. The best growth was achieved at 30 °C and pH 8–10 in medium containing 0.5 % (w/v) NaCl. The cell-wall peptidoglycan of strain OMN17 was type A4α; ( Lys–Gly- Asp) and the cell-wall sugars were ribose, traces of galactose and arabinose. The only respiratory quinone found in strain OMN17 was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C and anteiso-C. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis of strain OMN17 based on the 16S rRNA gene sequence showed that the strain was most closely related to (97.8 %), (97.5 %), (97.4 %) and (97.0 %). The DNA–DNA hybridization results indicated that this strain was distinct from other species of the genus , the degree of relatedness being 21.8 ± 0.2 % with , 45.6 ± 1.8 % with , 33.7 ± 1.2 % with and 23.7 ± 0.7 % with . The DNA G+C content of strain OMN17 was 38.1 mol%. The phenotypic, chemotaxonomic and genetic analyses identified strain OMN17 as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OMN17 ( = DSM 28019 = CCTCC AB 2014073). An emended description of the genus is also provided.

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2015-08-01
2024-04-18
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References

  1. Ahmed I., Yokota A., Yamazoe A., Fujiwara T. ( 2007;). Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. Int J Syst Evol Microbiol 57 1117–1125 [View Article] [PubMed].
     [Google Scholar]
  2. Claus D., Berkeley R.C.W. ( 1986;). Genus Bacillus Cohn 1872. . In Bergey's Manual of Systematic Bacteriology vol. 2, pp. 1105–1139. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore, MD: Williams & Wilkins;.
     [Google Scholar]
  3. Conn H.J., Bartholomew J.W., Jennison M.W. ( 1957;). Staining methods. . In Manual of Microbial Methods, pp. 30–60. Edited by Society of American Bacteriologists. New York: McGraw-Hill;.
     [Google Scholar]
  4. Cowan S.T., Steel K.J. ( 1965). Manual of the Identification of Medical Bacteria London: Cambridge University Press;.
     [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133–142 [View Article] [PubMed].
     [Google Scholar]
  6. Duan Y.Q., He S.T., Li Q.Q., Wang M.F., Wang W.Y., Zhe W., Cao Y.H., Mo M.H., Zhai Y.L., Li W.J. ( 2013;). Lysinibacillus tabacifolii sp. nov., a novel endophytic bacterium isolated from Nicotiana tabacum leaves. J Microbiol 51 289–294 [View Article] [PubMed].
     [Google Scholar]
  7. Glazunova O.O., Raoult D., Roux V. ( 2006;). Bacillus massiliensis sp. nov., isolated from cerebrospinal fluid. Int J Syst Evol Microbiol 56 1485–1488 [View Article] [PubMed].
     [Google Scholar]
  8. Gordon R.E., Barnett D.A., Handerhan J.E., Pang C.-N. ( 1974;). Nacardia coeliaca Nacardia autotrophica, and the nocardin strain. Int J Syst Evol Microbiol 24 54–63.
     [Google Scholar]
  9. Jung M.Y., Kim J.S., Paek W.K., Styrak I., Park I.S., Sin Y., Paek J., Park K.A., Kim H., authors other. ( 2012;). Description of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis Bacillus odysseyi to the genus Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended description of the genus Lysinibacillus . Int J Syst Evol Microbiol 62 2347–2355 [View Article] [PubMed].
     [Google Scholar]
  10. Kämpfer P., Martin K., Glaeser S.P. ( 2013;). Lysinibacillus contaminans sp. nov., isolated from surface water. Int J Syst Evol Microbiol 63 3148–3153 [View Article] [PubMed].
     [Google Scholar]
  11. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., authors other. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  12. Kim S.J., Jang Y.H., Hamada M., Ahn J.H., Weon H.Y., Suzuki K., Whang K.S., Kwon S.W. ( 2013;). Lysinibacillus chungkukjangi sp. nov., isolated from Chungkukjang, Korean fermented soybean food. J Microbiol 51 400–404 [View Article] [PubMed].
     [Google Scholar]
  13. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  14. Kong D., Wang Y., Zhao B., Li Y., Song J., Zhai Y., Zhang C., Wang H., Chen X., authors other. ( 2014;). Lysinibacillus halotolerans sp. nov., isolated from saline-alkaline soil. Int J Syst Evol Microbiol 64 2593–2598 [View Article] [PubMed].
     [Google Scholar]
  15. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 1–67 [View Article].
     [Google Scholar]
  16. Lee C.S., Jung Y.T., Park S., Oh T.K., Yoon J.H. ( 2010;). Lysinibacillus xylanilyticus sp. nov., a xylan-degrading bacterium isolated from forest humus. Int J Syst Evol Microbiol 60 281–286 [View Article] [PubMed].
     [Google Scholar]
  17. Liu H., Song Y., Chen F., Zheng S., Wang G. ( 2013;). Lysinibacillus manganicus sp. nov., isolated from manganese mining soil. Int J Syst Evol Microbiol 63 3568–3573 [View Article] [PubMed].
     [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  19. Miwa H., Ahmed I., Yokota A., Fujiwara T. ( 2009;). Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil. Int J Syst Evol Microbiol 59 1427–1432 [View Article] [PubMed].
     [Google Scholar]
  20. Schumann P. ( 2011;). Peptidoglycan structure. Methods Microbiol 38 101–129 [View Article].
     [Google Scholar]
  21. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhart P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  23. Timke M., Wang-Lieu N.Q., Altendorf K., Lipski A. ( 2005;). Community structure and diversity of biofilms from a beer bottling plant as revealed using 16S rRNA gene clone libraries. Appl Environ Microbiol 71 6446–6452 [View Article] [PubMed].
     [Google Scholar]
  24. Tindall B.J., Sikorski J., Smibert R.M., Kreig N.R. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology , 3rd edn., pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington DC: American Society for Microbiology;.
     [Google Scholar]
  25. You Z.Q., Li J., Qin S., Tian X.P., Wang F.Z., Zhang S., Li W.J. ( 2013;). Bacillus abyssalis sp. nov., isolated from a sediment of the South China Sea. Antonie van Leeuwenhoek 103 963–969 [View Article] [PubMed].
     [Google Scholar]
  26. Zhao F., Feng Y.Z., Chen R.R., Zhang H.Y., Wang J.H., Lin X.G. ( 2014;). Bacillus fengqiuensis sp. nov., isolated from a typical sandy loam soil under long-term fertilization. Int J Syst Evol Microbiol 64 2849–2856 [View Article] [PubMed].
     [Google Scholar]
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Lysinibacillus alkaliphilus sp. nov., an extremely alkaliphilic bacterium, and emended description of genus Lysinibacillus
Int J Syst Evol Microbiol 65, 2426 (2015); https://doi.org/10.1099/ijs.0.000280
/content/journal/ijsem/10.1099/ijs.0.000280
/content/journal/ijsem/10.1099/ijs.0.000280
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