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Abstract

An extremely halophilic archaeon, strain DA50, was isolated from a brine sample of Urmia lake, a hypersaline environment in north-west Iran. Strain DA50 was orange-pigmented, motile, pleomorphic and required at least 2.5 M NaCl but not MgCl for growth. Optimal growth was achieved at 4.0 M NaCl and 0.3 M MgCl. The optimum pH and temperature for growth were pH 7.0 and 45 °C, while it was able to grow over a pH range of 6.5–8.0 and a temperature range of 25–50 °C. Analysis of 16S rRNA gene sequences revealed that strain DA50 is a member of the family , showing a low level of similarity with other members of this family. Highest similarities, 94.4, 94.0 and 93.9 %, were obtained with the 16S rRNA gene sequences of the type strains of , and , respectively. Polar lipid analyses revealed that strain DA50 contains phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. Four unidentified glycolipids and two minor phospholipids were also observed. The only quinone present was MK-8(II-H). The G+C content of its DNA was 62.3 mol%. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain DA50 is thus considered to represent a novel species of a new genus within the family , order , for which the name gen. nov., sp. nov. is proposed. The type strain of is DA50 ( = IBRC-M 10912 = CECT 8510).

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2015-08-01
2021-04-20
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