1887

Abstract

A Gram-stain-negative, diesel-oil-degrading, rod-shaped bacterium (designated JC234) was isolated from a water sample collected from diesel-oil-contaminated backwaters in Kerala, India. Strain JC234 was oxidase- and catalase-positive, and grew at 20–35 °C and at pH 7–9. Cells contained bacteriochlorophyll-, hydroxydemethylspheroidene and three unidentified carotenoids. Growth occurred under aerobic, microaerobic and phototrophic anaerobic conditions. Strain JC234 could utilize diesel-oil as a sole source of carbon and energy. Based on the 16S rRNA gene sequence analysis, strain JC234 belonged to the genus within the family , and was closely related to AM1V30 (98.1 % 16S rRNA gene sequence similarity), JG120-1 (97.6 %) and other members of the genus ( < 96.4 %). Strain JC234 showed 22 ± 2 % and 28 ± 1.5 % DNA–DNA hybridization with KCTC 22096 and KCTC 23107, respectively. The DNA G+C content of strain JC234 was 54.3 mol %. The major cellular fatty acids were Cω7/Cω6, C and Cω7/Cω6. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol were the major polar lipids. Strain JC234 contained Q10 as the predominant ubiquinone. On the basis of morphological, physiological, genetic, phylogenetic and chemotaxonomical analyses, we conclude that strain JC234 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC234 ( = KCTC 42071 = LMG 28200). An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000277
2015-08-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2403.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000277&mimeType=html&fmt=ahah

References

  1. Ahrens R. . ( 1968;). Taxonomische Untersuchungen an sternbildenden Agrobacterium-Arten aus der westlichen Ostsee. Kiel Meeresforsch 24: 147–173 (in German).
    [Google Scholar]
  2. Altschul S.F. , Madden T.L. , Schäffer A.A. , Zhang J. , Zhang Z. , Miller W. , Lipman D.J. . ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402 [CrossRef] [PubMed].
    [Google Scholar]
  3. Biebl H. , Tindall B.J. , Pukall R. , Lünsdorf H. , Allgaier M. , Wagner-Döbler I. . ( 2006;). Hoeflea phototrophica sp. nov., a novel marine aerobic alphaproteobacterium that forms bacteriochlorophyll a . Int J Syst Evol Microbiol 56: 821–826 [CrossRef] [PubMed].
    [Google Scholar]
  4. Cappuccino J.G. , Sherman N. . ( 1998;). Microbiology: a Laboratory Manual , 5th edition. Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  5. Chung E.J. , Park J.A. , Pramanik P. , Bibi F. , Jeon C.O. , Chung Y.R. . ( 2013;). Hoeflea suaedae sp. nov., an endophytic bacterium isolated from the root of the halophyte Suaeda maritima . Int J Syst Evol Microbiol 63: 2277–2281 [CrossRef] [PubMed].
    [Google Scholar]
  6. Greub G. , Raoult D. . ( 2003;). Rhodobacter massiliensis sp. nov., a new amoebae-resistant species isolated from the nose of a patient. Res Microbiol 154 154: 631–635 [CrossRef] [PubMed].
    [Google Scholar]
  7. Hanada S. , Takaichi S. , Matsuura K. , Nakamura K. . ( 2002;). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52: 187–193 [PubMed].[CrossRef]
    [Google Scholar]
  8. Helsel L.O. , Hollis D. , Steigerwalt A.G. , Morey R.E. , Jordan J. , Aye T. , Radosevic J. , Jannat-Khah D. , Thiry D. , other authors . ( 2007;). Identification of “Haematobacter,” a new genus of aerobic Gram-negative rods isolated from clinical specimens, and reclassification of Rhodobacter massiliensis as “Haematobacter massiliensis comb. nov.”. J Clin Microbiol 45: 1238–1243 [CrossRef] [PubMed].
    [Google Scholar]
  9. Imhoff J.F. , Caumette P. . ( 2004;). Recommended standards for the description of new species of anoxygenic phototrophic bacteria. Int J Syst Evol Microbiol 54: 1415–1421 [CrossRef] [PubMed].
    [Google Scholar]
  10. Jung M.-Y. , Shin K.-S. , Kim S. , Kim S.-J. , Park S.-J. , Kim J.-G. , Cha I.-T. , Kim M.-N. , Rhee S.-K. . ( 2013;). Hoeflea halophila sp. nov., a novel bacterium isolated from marine sediment of the East Sea, Korea. Antonie van Leeuwenhoek 103: 971–978 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kim O.S. , Cho Y.J. , Lee K. , Yoon S.H. , Kim M. , Na H. , Park S.C. , Jeon Y.S. , Lee J.H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  13. Lakshmi K.V.N.S. , Sasikala Ch. , Ashok Kumar G.V. , Chandrasekaran R. , Ramana Ch.V. . ( 2011;). Phaeovibrio sulfidiphilus gen. nov., sp. nov., phototrophic alphaproteobacteria isolated from brackish water. Int J Syst Evol Microbiol 61: 828–833 [CrossRef] [PubMed].
    [Google Scholar]
  14. Marmur J. . ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208–218 [CrossRef].
    [Google Scholar]
  15. Mesbah M. , Premachandran U. , Whitman W.B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  16. Nagashima K.V.P. , Hiraishi A. , Shimada K. , Matsuura K. . ( 1997;). Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria. J Mol Evol 45: 131–136 [CrossRef] [PubMed].
    [Google Scholar]
  17. Oren A. , Duker S. , Ritter S. . ( 1996;). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138: 135–140 [CrossRef].
    [Google Scholar]
  18. Palacios L. , Arahal D.R. , Reguera B. , Marín I. . ( 2006;). Hoeflea alexandrii sp. nov., isolated from the toxic dinoflagellate Alexandrium minutum AL1V. Int J Syst Evol Microbiol 56: 1991–1995 [CrossRef] [PubMed].
    [Google Scholar]
  19. Peix A. , Rivas R. , Trujillo M.E. , Vancanneyt M. , Velázquez E. , Willems A. . ( 2005;). Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 55: 1163–1166 [CrossRef] [PubMed].
    [Google Scholar]
  20. Rüger H.J. , Höfle M.G. . ( 1992;). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42: 133–143 [CrossRef] [PubMed].
    [Google Scholar]
  21. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  22. Schaeffer A.B. , Fulton M.D. . ( 1933;). A Simplified method of staining endospores. Science 77: 194 [CrossRef] [PubMed].
    [Google Scholar]
  23. Seldin L. , Dubnau D. . ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35: 151–154 [CrossRef].
    [Google Scholar]
  24. Smibert R.M. , Krieg N.R. . ( 1981;). General characterization. . In Manual of Methods for General Bacteriology, pp. 409–443. Edited by Gerhardt P , Murray R. G. E , Costilow R. N , Nester E. W , Wood W. A , Krieg N. R , Phillips G. B . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  25. Stackebrandt E. , Goebel B.M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  26. Stevenson B.S. , Suflita M.T. , Stamps B.W. , Moore E.R.B. , Johnson C.N. , Lawson P.A. . ( 2011;). Hoeflea anabaenae sp. nov., an epiphytic symbiont that attaches to the heterocysts of a strain of Anabaena . Int J Syst Evol Microbiol 61: 2439–2444 [CrossRef] [PubMed].
    [Google Scholar]
  27. Subhash Y. , Tushar L. , Sasikala Ch. , Ramana Ch.V. . ( 2013;). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. Int J Syst Evol Microbiol 63: 2132–2137 [CrossRef] [PubMed].
    [Google Scholar]
  28. Subhash Y. , Sasikala Ch. , Ramana Ch.V. . ( 2014;). Bacillus luteus sp. nov., isolated from soil. Int J Syst Evol Microbiol 64: 1580–1586 [CrossRef] [PubMed].
    [Google Scholar]
  29. Suresh G. , Sasikala Ch. , Ramana Ch.V. . ( 2015;). Reclassification of Gemmobacter changlensis to a new genus as Cereibacter changlensis gen. nov., comb. nov. Int J Syst Evol Microbiol 65: 794–798 [CrossRef] [PubMed].
    [Google Scholar]
  30. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  31. Tindall B.J. . ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  32. Tindall B.J. , Tomlinson G.A. , Hochstein L.I. . ( 1987;). Polar lipid composition of a new halobacterium. Syst Appl Microbiol 9: 6–8 [CrossRef] [PubMed].
    [Google Scholar]
  33. Tourova T.P. , Antonov A.S. . ( 1987;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19: 333–355 [CrossRef].
    [Google Scholar]
  34. Trüper H.G. , Pfennig N. . ( 1981;). Isolation of members of the families Chromatiaceae Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279–289. Edited by Starr M. P , Stolp H , Trüper H. G , Balows A , Schlegel H. G . Berlin: Springer;.
    [Google Scholar]
  35. Xie C.H. , Yokota A. . ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49: 345–349 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000277
Loading
/content/journal/ijsem/10.1099/ijs.0.000277
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error