1887

Abstract

A Gram-stain-negative, diesel-oil-degrading, rod-shaped bacterium (designated JC234) was isolated from a water sample collected from diesel-oil-contaminated backwaters in Kerala, India. Strain JC234 was oxidase- and catalase-positive, and grew at 20–35 °C and at pH 7–9. Cells contained bacteriochlorophyll-, hydroxydemethylspheroidene and three unidentified carotenoids. Growth occurred under aerobic, microaerobic and phototrophic anaerobic conditions. Strain JC234 could utilize diesel-oil as a sole source of carbon and energy. Based on the 16S rRNA gene sequence analysis, strain JC234 belonged to the genus within the family , and was closely related to AM1V30 (98.1 % 16S rRNA gene sequence similarity), JG120-1 (97.6 %) and other members of the genus ( < 96.4 %). Strain JC234 showed 22 ± 2 % and 28 ± 1.5 % DNA–DNA hybridization with KCTC 22096 and KCTC 23107, respectively. The DNA G+C content of strain JC234 was 54.3 mol %. The major cellular fatty acids were Cω7/Cω6, C and Cω7/Cω6. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol were the major polar lipids. Strain JC234 contained Q10 as the predominant ubiquinone. On the basis of morphological, physiological, genetic, phylogenetic and chemotaxonomical analyses, we conclude that strain JC234 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC234 ( = KCTC 42071 = LMG 28200). An emended description of the genus is also provided.

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2015-08-01
2024-10-06
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