1887

Abstract

Strawberry red leaf phytoplasma was found in strawberry plants from production fields in Lules (Tucumán province) and Bella Vista (Corrientes province), Argentina. Characteristic strawberry red leaf symptoms were stunting, young leaves with yellowing at the edges, mature leaves which curled and were reddish at the abaxial face, flower and fruit deformation and death. The pathogen was detected with phytoplasma-universal primer pairs P1/P7 followed by R16F2n/R16R2 as nested primers in 13 diseased plants. Based on RFLP and sequence analysis of the amplified 16S rRNA gene, the phytoplasma was related to the 16SrXIII group (Mexican periwinkle virescence). the RFLP profile of all the samples analysed revealed the presence of a unique pattern, showing that the novel phytoplasma is different from all the phytoplasmas currently composing the 16SrXIII group. The phylogenetic analysis was consistent with RFLP analysis as the strawberry red leaf phytoplasma was grouped within the 16SrXIII group, but formed a particular cluster. On this basis, the Strawberry red leaf phytoplasma associated with strawberry red leaf disease was assigned to a new subgroup, 16SrXIII-F.

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2015-08-01
2019-10-14
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References

  1. Amarel-Mello A. , Bedendo I.P. , Kitajima E.W. , Ribeiro L.F.C. , Kobori R. . ( 2006;). Tomato big bud associated with a phytoplasma belonging to group 16SrIII in Brazil. Int J Pest Manage 52: 233–237 [CrossRef].
    [Google Scholar]
  2. Amarel-Mello A.P. , Eckstein B. , Flores D. , Kreyci P.F. , Bedendo I.P. . ( 2011;). Identification by computer-simulated RFLP of phytoplasmas associated with eggplant giant calyx representative of two subgroups, a lineage of 16SrIII-J and the new subgroup 16SrIII-U. Int J Syst Evol Microbiol 61: 1454–1461 [CrossRef] [PubMed].
    [Google Scholar]
  3. Arneodo J. , Galdeano E. , Orrego A. , Stauffer A. , Nome S. , Conci L. . ( 2005;). Identification of two phytoplasmas detected in China-trees with decline symptoms in Paraguay. Australas Plant Pathol 34: 583–585 [CrossRef].
    [Google Scholar]
  4. Arneodo J. , Marini D.C. , Galdeano E. , Meneguzzi N. , Bacci M. , Domecq C. , Nome S.F. , Conci L. . ( 2007;). Diversity and geographical distribution of phytoplasmas infecting China-tree in Argentina. J Phytopathol 155: 70–75 [CrossRef].
    [Google Scholar]
  5. Arocha Y. , López M. , Piñol B. , Fernández M. , Picornell B. , Almeida R. , Palenzuela I. , Wilson M.R. , Jones P. . ( 2005;). Candidatus Phytoplasma graminis’ and ‘Candidatus Phytoplasma caricae’, two novel phytoplasmas associated with diseases of sugarcane, weeds and papaya in Cuba. Int J Syst Evol Microbiol 55: 2451–2463 [CrossRef] [PubMed].
    [Google Scholar]
  6. Barros T.S.L. , Davis R.E. , Resende R. , Dally E. . ( 2002;). Erigeron witches’-broom phytoplasma in Brazil represents rew subgroup VII-B in 16S rRNA gene group VII, the ash yellows phytoplasma group. Plant Dis 86: 1142–1148 [CrossRef].
    [Google Scholar]
  7. Bertaccini A. , Duduk B. , Paltrinieri S. , Contaldo N. . ( 2014;). Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. American Journal of Plant Sciences 5: 1763–1788 [CrossRef].
    [Google Scholar]
  8. Conci L. , Meneguzzi N. , Galdeano E. , Torres L. , Nome C. , Nome S. . ( 2005;). Detection and molecular characterization of an alfalfa phytoplasma in Argentina that represents a new subgroup in the 16S rDNA ash yellows group ‘Candidatus Phytoplasma fraxin’). Eur J Plant Pathol 113: 255–265 [CrossRef].
    [Google Scholar]
  9. Davis R.E. , Lee I.-M. . ( 2000;). Phytoplasma. . In Encyclopedia of Microbiology , 2nd edn., pp. 640–646. Edited by Lederberg J. , other authors . New York: Academic Press;.
    [Google Scholar]
  10. Davis R.E. , Zhao Y. , Dally E.L. , Jomantiene R. , Lee I.M. , Wei W. , Kitajima E.W. . ( 2012;). Candidatus Phytoplasma sudamericanum’, a novel taxon, and strain PassWB-Br4, a new subgroup 16SrIII-V phytoplasma, from diseased passion fruit (Passiflora edulis f. flavicarpa Deg.). Int J Syst Evol Microbiol 62: 984–989 [CrossRef] [PubMed].
    [Google Scholar]
  11. Deng S. , Hiruki D. . ( 1991;). Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. J Microbiol Methods 14: 53–61 [CrossRef].
    [Google Scholar]
  12. Doyle J.J. . ( 1990;). Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
    [Google Scholar]
  13. Eckstein B. , Barbosa J.C. , Kreyci P.F. , Canale M.C. , Brunelli K.R. , Bedendo I.P. . ( 2013;). Broccoli stunt, a new disease in broccoli plants associated with three distinct phytoplasma groups in Brazil. J Phytopathol 161: 442–444 [CrossRef].
    [Google Scholar]
  14. Fernández F.D. , Conci V.C. , Kirschbaum D.S. , Conci L.R. . ( 2013;). Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina. Eur J Plant Pathol 135: 1–4 [CrossRef].
    [Google Scholar]
  15. Fiore N. , Longone V. , González F. , Zamorano A. , Pino A.M. , Araya J. , Picciau L. , Alma A. , Paltrinieri S. , Contaldo N. , Bertaccini A. . ( 2012;). Transmission of 16SrIII-J phytoplasma by Paratanus exitiosus leafhopper. . In Proceedings of the 17th Congress of Virus and Virus-like Diseases (ICVG), Davis, CA, USA, October 7–14, 2012, pp. 230–231.
    [Google Scholar]
  16. Galdeano E. , Torres L.E. , Meneguzzi N. , Guzmán F. , Gomez G.G. , Docampo D.M. , Conci L. . ( 2004;). Molecular characterization of 16S ribosomal DNA and phylogenetic analysis of two X-disease group phytoplasmas affecting China-tree (Melia azedarach L.) and garlic (Allium sativum L.) in Argentina. J Phytopathol 152: 174–181 [CrossRef].
    [Google Scholar]
  17. Galdeano E. , Guzmán F. , Fernandez F. , Conci L. . ( 2013;). Genetic diversity of 16SrIII group phytoplasmas in Argentina. Predominance of subgroups 16SrIII-J and B and two new subgroups 16SrIII-W and X. Eur J Plant Pathol 137: 753–764 [CrossRef].
    [Google Scholar]
  18. Gundersen D.E. , Lee I.M. . ( 1996;). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 35: 144–151.
    [Google Scholar]
  19. Harrison N.A. , Legard D.E. , Di Bonito R. , Richardson P.A. . ( 1997;). Detection and differentiation of phytoplasmas associated with diseases of strawberry in Florida. Plant Dis 81: 230 [CrossRef].
    [Google Scholar]
  20. Harrison N. , Boa E. , Carpio M.L. . ( 2003;). Characterization of phytoplasmas detected in chinaberry trees with symptoms of leaf yellowing and decline in Bolivia. Plant Pathol 52: 147–157 [CrossRef].
    [Google Scholar]
  21. IRPCM Phytoplasma/Spiroplasma Working Team–Phytoplasma Taxonomy Group ( 2004;). Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54: 1243–1255 [CrossRef] [PubMed].
    [Google Scholar]
  22. Jomantiene R. , Davis R.E. , Maas J. , Dally E.L. . ( 1998;). Classification of new phytoplasmas associated with diseases of strawberry in Florida, based on analysis of 16S rRNA and ribosomal protein gene operon sequences. Int J Syst Bacteriol 48: 269–277 [CrossRef] [PubMed].
    [Google Scholar]
  23. Jomantiene R. , Maas J.L. , Dally E.L. , Davis R.E. . ( 1999;). First report of clover yellow edge and STRAWB2 phytoplasmas in strawberry in Maryland. Plant Dis 83: 1072 [CrossRef].
    [Google Scholar]
  24. Jomantiene R.J. , Maas L. , Davis R.E. , Dally E.L. . ( 2001;). Molecular identification and classification of a phytoplasma associated with phyllody of strawberry fruit in Maryland. Plant Dis 85: 335 [CrossRef].
    [Google Scholar]
  25. Kirschbaum D.S. , Honorato J. , Cantliffe D.J. . ( 2000;). Strawberry waiting bed plants: a valid alternative to increase early and total yields in sub-tropical regions. Scientia Horticulturae 84: 83–90.[CrossRef]
    [Google Scholar]
  26. Kirschbaum D.S. , Real E. , Borquez A.M. . ( 2012;). Evolution of Argentina's strawberry transplant exports in the last decade. . In VII International Strawberry Symposium, 2012, Beijing, China, p. 184.
    [Google Scholar]
  27. Lee I.-M. , Gundersen-Rindal D.E. , Davis R.E. , Bartoszyk I.-M. . ( 1998;). Revised classification scheme of phytoplasmas based on RFLP analysis of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48: 1153–1169 [CrossRef].
    [Google Scholar]
  28. Lee I.-M. , Davis R.E. , Gundersen-Rindal D.E. . ( 2000;). Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54: 221–255 [CrossRef] [PubMed].
    [Google Scholar]
  29. Lee I.-M. , Bottner K.D. , Secor G. , Rivera-Varas V. . ( 2006;). Candidatus Phytoplasma americanum’, a phytoplasma associated with a potato purple top wilt disease complex. Int J Syst Evol Microbiol 56: 1593–1597 [CrossRef] [PubMed].
    [Google Scholar]
  30. Lee I.-M. , Bottner-Parker K.D. , Zhao Y. , Villalobos W. , Moreira L. . ( 2011;). Candidatus Phytoplasma costaricanum’ a novel phytoplasma associated with an emerging disease in soybean (Glycine max). Int J Syst Evol Microbiol 61: 2822–2826 [CrossRef] [PubMed].
    [Google Scholar]
  31. Maas J.L. . ( 1998;). Compendium of Strawberry Diseases , 2nd edn. St. Paul, MN: American Phytopathological Society;.
    [Google Scholar]
  32. Melo L. , Silva E. , Flôres D. , Ventura J. , Costa H. , Bedendo I. . ( 2013;). A phytoplasma representative of a new subgroup, 16SrXIII-E, associated with Papaya apical curl necrosis. Eur J Plant Pathol 137: 445–450 [CrossRef].
    [Google Scholar]
  33. Meneguzzi N. , Torres L.E. , Galdeano E. , Guzmán F.A. , Nome S.F. , Conci L.R. . ( 2008;). Molecular characterization of a phytoplasma of the ash yellows group (16Sr VII-B) occurring in Artemisia annua Conyza bonariensis weeds. AgriScientia 25: 7–15.
    [Google Scholar]
  34. Montano H.G. , Davis R.E. , Dally E.L. , Pimentel J.P. , Brioso P.S.T. . ( 2000;). Identification and phylogenetic analysis of a new phytoplasma from diseased chayote in Brazil. Plant Dis 84: 429–436 [CrossRef].
    [Google Scholar]
  35. Montano H.G. , Davis R.E. , Dally E.L. , Hogenhout S. , Pimentel J.P. , Brioso P.S. . ( 2001;). ‘Candidatus Phytoplasma brasiliense’, a new phytoplasma taxon associated with hibiscus witches’ broom disease. Int J Syst Evol Microbiol 51: 1109–1118 [CrossRef] [PubMed].
    [Google Scholar]
  36. Nejat N. , Vadamalai G. , Davis R.E. , Harrison N.A. , Sijam K. , Dickinson M. , Abdullah S.N. , Zhao Y. . ( 2013;). ‘Candidatus Phytoplasma malaysianum’, a novel taxon associated with virescence and phyllody of Madagascar periwinkle (Catharanthus roseus). Int J Syst Evol Microbiol 63: 540–548 [CrossRef] [PubMed].
    [Google Scholar]
  37. Padovan A. , Gibb K. , Persley D. . ( 2000;). Association of ‘Candidatus Phytoplasma australiense’ with green petal and lethal yellows diseases in strawberry. Plant Pathol 49: 362–369 [CrossRef].
    [Google Scholar]
  38. Quaglino F. , Zhao Y. , Casati P. , Bulgari D. , Bianco P.A. , Wei W. , Davis R.E. . ( 2013;). Candidatus Phytoplasma solani’, a novel taxon associated with stolbur- and bois noir-related diseases of plants. Int J Syst Evol Microbiol 63: 2879–2894 [CrossRef] [PubMed].
    [Google Scholar]
  39. Sambrook J. , Fritsch E.F. , Maniatis T. . ( 1989;). Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  40. Santos-Cervantes M.E. , Chávez-Medina J.A. , Acosta-Pardini J. , Flores-Zamora G.L. , Méndez-Lozano J. , Leyva-López N.E. . ( 2010;). Genetic diversity and geographical distribution of phytoplasmas associated with Potato purple top disease in Mexico. Plant Dis 94: 388–395 [CrossRef].
    [Google Scholar]
  41. Schneider B. , Seemueller E. , Smart C.D. , Kirkpatrick B. . ( 1995;). Phylogenetic classification of plant pathogenic mycoplasmalike organisms or phytoplasmas. . In Molecular and Diagnostic Procedures in Mycoplasmology vol. 1, pp. 369–380. Edited by Razin S. , Tully J. G. . San Diego, CA: Academic Press; [CrossRef].
    [Google Scholar]
  42. SENASA ( 2012;). Certificaciones del Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA) a productos de origen animal y vegetal. http://www.senasa.gov.ar/estadistica.php.
  43. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  44. Valiunas D. , Staniulis J. , Davis R.E. . ( 2006;). Candidatus Phytoplasma fragariae’, a novel phytoplasma taxon discovered in yellows diseased strawberry, Fragaria x ananassa . Int J Syst Evol Microbiol 56: 277–281 [CrossRef] [PubMed].
    [Google Scholar]
  45. Wei W. , Lee I.-M. , Davis R.E. , Suo X. , Zhao Y. . ( 2008;). Automated RFLP pattern comparison and similarity coefficient calculation for rapid delineation of new and distinct phytoplasma 16Sr subgroup lineages. Int J Syst Evol Microbiol 58: 2368–2377 [CrossRef] [PubMed].
    [Google Scholar]
  46. Zhao Y. , Wei W. , Lee I.-M. , Shao J. , Suo X. , Davis R.E. . ( 2009;). Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59: 2582–2593 [CrossRef] [PubMed].
    [Google Scholar]
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