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Volume 65, Issue Pt_8

sp. nov., isolated from seawater, and emended description of the genus Free

Abstract

A Gram-stain-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, white-pigmented and rod-shaped bacterium, designated strain XH122, was isolated from a surface seawater sample collected from the South Pacific Gyre (45° 58′ E 163° 11′ S) during Integrated Ocean Drilling Program Expedition 329.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH122 belonged to the genus Leucothrix and showed highest 16S rRNA gene sequence similarity to Leucothrix mucor DSM 2157 (94.3 %). It showed lower sequence similarities ( < 90.7 %) with all other representatives of the class . Optimal growth occurred in the presence of 2 % (w/v) NaCl, at pH 8.0 and at 28 °C. The DNA G+C content of strain XH122 was 46.2 mol%. The major fatty acids were C, Cω9 and Cω9. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of data from this polyphasic study, strain XH122 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XH122 ( = DSM 25984 = JCM 18388).

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2015-08-01
2024-04-16
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References

  1. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K.(editors). ( 1995). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology , 3rd edn. New York: Wiley;.
     [Google Scholar]
  2. Bernardet J.F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 1049–1070 [View Article] [PubMed] .
     [Google Scholar]
  3. Beveridge T., Lawrence J., Murray R. ( 2007;). Sampling and staining for light microscopy Methods for General and Molecular Microbiology. In Methods for General and Molecular Microbiology, pp. 19–33. Edited by Reddy C. A, Beveridge T. J, Breznak J. A, Marzluf G, Schmidt T. M, Snyder L. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  4. Brock T.D. ( 1974;). Family IV. Leucotrichaceae. Buchanan 1957. . In Bergey's Manual of Determinative Bacteriology , 8th edn., pp. 118–119. Edited by Buchanan R. E, Gibbons N. E. Baltimore: Williams & Wilkins;.
     [Google Scholar]
  5. Brock T.D. ( 2006;). The genus Leucothrix . . In The Prokaryotes , 3rd edn., pp. 931–938. Edited by Dworkin M, Schleifer K. H. New York: Springer; [View Article].
     [Google Scholar]
  6. Buchanan R.E. ( 1957;). Family III. Leucotrichaceae fam. nov. . In Bergey's Manual of Determinative Bacteriology , 7th edn., pp. 850–851. Edited by Breed R. S, Murray E. G. D, Smith N. R. Baltimore: Williams & Wilkins;.
     [Google Scholar]
  7. Collins M.D., Shah H.N. ( 1984;). Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa . Arch Microbiol 137 247–249 [View Article].
     [Google Scholar]
  8. Dul'tseva N.M., Dubinina G.A., Lysenko A.M. ( 1996;). [Bioantimutagenic effect of the culture fluid of Streptococcus faecalis on mutagenesis induced by 2-nitrofluorene in Salmonella typhimurium TA 1538 and TA 98]. Microbiologiia 65 79–87 [in Russian] [PubMed] .
     [Google Scholar]
  9. Garrity G.M., Bell J.A., Lilburn T. ( 2005;). Family I. Thiotrichaceae fam. nov. . In Bergey's Manual of Systematic Bacteriology , 2nd edn., pp. 131–210. Edited by Garrity B. M, Brenner D. J, Krieg N. R, Staley J. T. New York: Springer; [View Article].
     [Google Scholar]
  10. Grabovich M.Iu., Dul'tseva N.M., Dubinina G.A. ( 2002;). [Carbon and sulfur metabolism in representatives of two clusters of bacteria of the genus Leucothrix: a comparative study]. Mikrobiologiia 71 301–307 [in Russian] [PubMed] .
     [Google Scholar]
  11. Harold R., Stanier R.Y. ( 1955;). The genera Leucothrix Thiothrix . Bacteriol Rev 19 49–64 [PubMed].
     [Google Scholar]
  12. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed] .
     [Google Scholar]
  13. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed] .
     [Google Scholar]
  14. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 1–207.
     [Google Scholar]
  15. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479 297–306 [View Article] [PubMed] .
     [Google Scholar]
  16. Minnikin D., O'Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  17. Montero-Calasanz M., del C., Göker M., Rohde M., Spröer C., Schumann P., Busse H.J., Schmid M., Tindall B.J., Klenk H.P., Camacho M. ( 2013;). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii Chryseobacterium indologenes Chryseobacterium wanjuense Chryseobacterium gregarium . Int J Syst Evol Microbiol 63 4386–4395 [View Article] [PubMed] .
     [Google Scholar]
  18. Moore E.R.B., Arnscheidt A., Krüger A., Strömpl C., Mau M. ( 1999;). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Molecular Microbial Ecology Manual 1.6.1, pp. 1–15. Edited by Akkermans A. D. L, van Elsas J. D, de Bruijn F. J. Dordrecht: Kluwer;.
     [Google Scholar]
  19. Oersted A.S. ( 1844). De regionibus marinis, elementa topographiae historiconaturalis freti Oeresund Copenhagen: J.C. Scharling;.
     [Google Scholar]
  20. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed] .
     [Google Scholar]
  22. Tanaka N., Romanenko L.A., Iino T., Frolova G.M., Mikhailov V.V. ( 2011;). Cocleimonas flava gen. nov., sp. nov., a gammaproteobacterium isolated from sand snail (Umbonium costatum). Int J Syst Evol Microbiol 61 412–416 [View Article] [PubMed] .
     [Google Scholar]
  23. Teske A., Salman V. ( 2014;). The family Leucotrichaceae. . In The Prokaryotes: Gammaproteobacteria, pp. 391–409. Edited by Rosenberg E, DeLong E. F, Lory S, Stackebrandt E, Thompson F. Berlin: Springer;.
     [Google Scholar]
  24. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed] .
     [Google Scholar]
  25. Tindall B.J., Sikorski J., Smibert R.M., Krieg N.R. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A, Beveridge T. J, Breznak J. A, Marzluf G, Schmidt T. M, Snyder L. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  26. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697–703 [PubMed].
     [Google Scholar]
  27. Yoon J.H., Lee K.C., Kho Y.H., Kang K.H., Kim C.J., Park Y.H. ( 2002;). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52 123–130 [PubMed]. [CrossRef]
    [Google Scholar]
  28. Zhang Z., Yu T., Xu T., Zhang X.H. ( 2014;). Aquimarina pacifica sp. nov., isolated from seawater. Int J Syst Evol Microbiol 64 1991–1997 [View Article] [PubMed] .
     [Google Scholar]
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Leucothrix pacifica sp. nov., isolated from seawater, and emended description of the genus Leucothrix
Int J Syst Evol Microbiol 65, 2397 (2015); https://doi.org/10.1099/ijs.0.000270
/content/journal/ijsem/10.1099/ijs.0.000270
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