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Abstract

A novel Gram-stain-positive, coccoid, non-motile bacterium, designated strain AMV4, was isolated from a soil sample collected from a mud volcano located in the Andaman Islands, India. The colony was pale orange. Strain AMV4 was positive for oxidase, aesculinase, lysine decarboxylase and ornithine decarboxylase activities and negative for amylase, catalase, cellulase, protease, urease and lipase activities. 16S rRNA gene sequence analysis indicated that strain AMV4 was a member of the order and was closely related to with a sequence similarity of 97.13 % (pairwise alignment). Phylogenetic analyses showed that strain AMV4 clustered with and was distantly related to the other genera of the family . DNA–DNA hybridization between strains AMV4 and IV-75 showed a relatedness of 28 %. The predominant cellular fatty acids were iso-C (6.9 %), anteiso-C (25.3 %), C (12.9 %), anteiso-C (5.6 %), Cω9 (19.8 %) and Cω6,9,12 (9.1 %). The diagnostic diamino acid in the cell-wall peptidoglycan of strain AMV4 was -diaminopimelic acid. Strain AMV4 contained MK-10(H) as the predominant respiratory quinone. The polar lipids consisted of phosphatidylglycerol, one unidentified glycolipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content of strain AMV4 was 74.3 mol%. Based on data from this taxonomic study using a polyphasic approach, it is proposed that strain AMV4 represents a novel species of the genus , with the suggested name sp. nov. The type strain is AMV4 ( = CCUG 58430 = DSM 22863 = NBRC 107137).

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2015-08-01
2019-10-20
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