1887

Abstract

Strain T258 was isolated from forest soil at Bongnae Falls, South Korea. The strain exhibited antimicrobial and antifungal activity against the following strains: , , , , , and . Growth occurred on all ISP media tested (2, 3, 4, 5, 6 and 7), Czapek–Dox agar, potato dextrose agar, trypticase soy agar, Bennett's modified agar and nutrient agar at 28 °C. Aerial spores were produced solely on ISP Medium 4; the colour of the aerial mycelium was white and the substrate mycelium was ivory. Melanin production was negative on peptone–yeast extract iron agar (ISP Medium 6). The cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates contained glucose, ribose and galactose. The predominant menaquinones were MK-9(H) and MK-9(H) while the minor menaquinone was MK-10(H). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids (>10 %) were C (29.8 %), summed feature 3 (Cω7 and/or Cω6) (15.1 %), anteiso-C (13.5 %) and iso-C (10.3 %). DNA–DNA similarity with other strains ranged between 37.84 ± 1.15 % and 50.25 ± 1.91 %. On the basis of these data, we suggest that strain T258 represents a novel species that belong to the genus , for which we propose a name sp. nov. The type strain is T258 ( = KACC 18247 = KEMB 9005-212 = NBRC 110905).

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2015-08-01
2021-10-24
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