1887

Abstract

Phenotypic and phylogenetic studies were performed on new isolates of a novel Gram-stain-positive, anaerobic, non-sporulating, rod-shaped bacterium isolated from a thermophilic biogas plant. The novel organisms were able to degrade crystalline cellulose. 16S rRNA gene comparative sequence analysis demonstrated that the isolates formed a hitherto unknown subline within the family . As a representative of the whole group of isolates, strain T3/55 was further characterized. The closest relative of T3/55 among the taxa with validly published names is , sharing 93.9 % 16S rRNA gene sequence similarity. Strain T3/55 was catalase-negative, indole-negative, and produced acetate, ethanol and propionic acid as major end products from cellulose metabolism. The major cellular fatty acids (>1 %) were 16 : 0 dimethyl acetal, 16 : 0 fatty acid methyl ester and 16 : 0 aldehyde. The DNA G+C content was 36.6 mol%. A novel genus and species, gen. nov., sp. nov., is proposed based on phylogenetic analysis and physiological properties of the novel isolate. Strain T3/55 ( = DSM 29228 = CECT 8801), represents the type strain of gen. nov., sp. nov.

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2015-08-01
2021-03-09
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