1887

Abstract

A Gram-reaction-negative, facultatively anaerobic and rod-shaped bacterium, designated strain JN14CK-3, was isolated from surface sediment of the Jiulong River of China and was characterized phenotypically and phylogenetically. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain JN14CK-3 belonged to the genus , with the highest sequence similarity (98.3 %) to FH5. By contrast, strain JN14CK-3 shared low 16S rRNA gene sequence similarities ( < 91.0 %) with other type strains. The sole respiratory quinone was MK-7.The polar lipids were phosphatidylethanolamine and several unidentified phospholipids and lipids. The major fatty acids were iso-C, iso-C, anteiso-C, C 2-OH, iso-C 3-OH and iso-C 3-OH. The G+C content of the genomic DNA was 40.9 mol%. The digital DNA–DNA hybridization value and average nucleotide identity (ANI) between strain JN14CK-3 and FH5 were 34.2 ± 2.5 % and 87.1 %, respectively. The combined genotypic and phenotypic data showed that strain JN14CK-3 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain JN14CK-3 ( = MCCC 1A00734 = KCTC 42152).

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2015-07-01
2020-01-20
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