1887

Abstract

A Gram-reaction-negative, facultatively anaerobic and rod-shaped bacterium, designated strain JN14CK-3, was isolated from surface sediment of the Jiulong River of China and was characterized phenotypically and phylogenetically. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain JN14CK-3 belonged to the genus , with the highest sequence similarity (98.3 %) to FH5. By contrast, strain JN14CK-3 shared low 16S rRNA gene sequence similarities ( < 91.0 %) with other type strains. The sole respiratory quinone was MK-7.The polar lipids were phosphatidylethanolamine and several unidentified phospholipids and lipids. The major fatty acids were iso-C, iso-C, anteiso-C, C 2-OH, iso-C 3-OH and iso-C 3-OH. The G+C content of the genomic DNA was 40.9 mol%. The digital DNA–DNA hybridization value and average nucleotide identity (ANI) between strain JN14CK-3 and FH5 were 34.2 ± 2.5 % and 87.1 %, respectively. The combined genotypic and phenotypic data showed that strain JN14CK-3 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain JN14CK-3 ( = MCCC 1A00734 = KCTC 42152).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000260
2015-07-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2310.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000260&mimeType=html&fmt=ahah

References

  1. Collins M. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  2. Du Z.J., Wang Y., Dunlap C., Rooney A.P., Chen G.J. ( 2014;). Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64 16901696. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376. [CrossRef]
    [Google Scholar]
  4. Goris J., Konstantinidis K.T., Klappenbach J.A., Coenye T., Vandamme P., Tiedje J.M. ( 2007;). DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57 8191. [CrossRef]
    [Google Scholar]
  5. Iino T., Mori K., Itoh T., Kudo T., Suzuki K., Ohkuma M. ( 2014;). Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov. Int J Syst Evol Microbiol 64 36603667. [CrossRef]
    [Google Scholar]
  6. Kates M. ( 1972;). Techniques of lipidology: isolation, analysis and identification of lipids. . In Laboratory Techniques in Biochemistry and Molecular Biology, pp. 269610. Edited by Work T. S., Work E. 3 Amsterdam: Elsevier;.
    [Google Scholar]
  7. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721. [CrossRef]
    [Google Scholar]
  8. Lai Q., Yuan J., Gu L., Shao Z. ( 2009;). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59 12781281. [CrossRef]
    [Google Scholar]
  9. Liu C., Shao Z. ( 2005;). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55 11811186. [CrossRef]
    [Google Scholar]
  10. Meier-Kolthoff J.P., Auch A.F., Klenk H.P., Göker M. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14 60. [CrossRef]
    [Google Scholar]
  11. Rzhetsky A., Nei M. ( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35 367375. [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425.
    [Google Scholar]
  13. Sambrook J., Russell D.W. ( 2001). Molecular Cloning: a Laboratory Mannual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  14. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;.
  15. Skerman V. ( 1967). A Guide to the Identification of the Genera of Bacteria: with Methods and Digests of Generic Characteristics , 2nd edn. Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739. [CrossRef]
    [Google Scholar]
  17. Thompson C.C., Chimetto L., Edwards R.A., Swings J., Stackebrandt E., Thompson F.L. ( 2013;). Microbial genomic taxonomy. BMC Genomics 14 913. [CrossRef]
    [Google Scholar]
  18. Tindall B., Sikorski J., Smibert R., Krieg N. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. , 3rd edn. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  19. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000260
Loading
/content/journal/ijsem/10.1099/ijs.0.000260
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error