RT Journal Article SR Electronic(1) A1 Huang, Hai-Dong A1 Wang, Wei A1 Ma, Ting A1 Li, Guo-Qiang A1 Liang, Feng-Lai A1 Liu, Ru-LinYR 2009 T1 Sphingomonas sanxanigenens sp. nov., isolated from soil JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 4 SP 719 OP 723 DO https://doi.org/10.1099/ijs.0.000257-0 PB Microbiology Society, SN 1466-5034, AB Strain NX02T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil, and its taxonomic position was investigated using a polyphasic approach. Chemotaxonomic analysis revealed that strain NX02T possessed Q-10 as the predominant ubiquinone, sym-homospermidine as the major polyamine and C18 : 1 ω7c, C16 : 0 and C14 : 0 2-OH as the major fatty acids. The main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and an unidentified glycolipid. The DNA G+C content was 66.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NX02T belongs to the α-4 subgroup of the Proteobacteria, exhibiting the highest sequence similarity with respect to Sphingomonas azotifigens NBRC 15497T (95.9 %), Sphingomonas pituitosa DSM 13101T (95.8 %) and Sphingomonas dokdonensis KCTC 12541T (95.8 %). On the basis of these results, strain NX02T represents a novel species of the genus Sphingomonas sensu stricto, for which the name Sphingomonas sanxanigenens sp. nov. is proposed. The type strain is NX02T (=DSM 19645T =CGMCC 1.6417T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000257-0