Strain NX02, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil, and its taxonomic position was investigated using a polyphasic approach. Chemotaxonomic analysis revealed that strain NX02 possessed Q-10 as the predominant ubiquinone, -homospermidine as the major polyamine and C 7, C and C 2-OH as the major fatty acids. The main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and an unidentified glycolipid. The DNA GC content was 66.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NX02 belongs to the -4 subgroup of the , exhibiting the highest sequence similarity with respect to NBRC 15497 (95.9 %), DSM 13101 (95.8 %) and KCTC 12541 (95.8 %). On the basis of these results, strain NX02 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NX02 (=DSM 19645 =CGMCC 1.6417).


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vol. , part 4, pp. 719 - 723

Two-dimensional TLC of polar lipids of strain NX02 . DPG, Diphosphatidylglycerol; PMME, phosphatidylmonomethylethanolamine; PE, phosphatidylethanolamine; SGL, sphingoglycolipid; PDME, phosphatidyldimethylethanolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; GL, unidentified glycolipid.


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