1887

Abstract

The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain, designated S11-41, was isolated from a sediment sample of the Yellow Sea in China. The strain was able to grow well over a wide temperature range (1–35 °C). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S11-41 was related to the members of the genus , sharing highest sequence similarities with the type strains of (98.2 %), (97.4 %) and (96.9 %). The genomic DNA G+C content of strain S11-41 was 39.2 mol%. The predominant cellular fatty acids were iso-C (13.2 %), iso-C 3-OH (12.8 %) and iso-C G (12.2 %). Phosphatidylethanolamine was identified as the major polar lipid. Combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain S11-41 is a representative of a novel species of the genus , for which we propose the name sp. nov. (type strain S11-41 = DSM 28135 = LMG 28076).

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2015-07-01
2019-10-17
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References

  1. Alarico S. , Rainey F.A. , Empadinhas N. , Schumann P. , Nobre M.F. , da Costa M.S. . ( 2002;). Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the α-1 subclass of the Proteobacteria . Syst Appl Microbiol 25: 198–206 doi:10.1078/0723-2020-00116 Medline.[CrossRef]
    [Google Scholar]
  2. Bernardet J.-F. , Bowman J.P. . ( 2006;). The genus Flavobacterium . . In The Prokaryotes: a Handbook on the Biology of Bacteria, pp. 481–531. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . 7 , 3rd edn.., New York: Springer;.
    [Google Scholar]
  3. Bowman J.P. , Nichols D.S. . ( 2005;). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55: 1471–1486 doi:10.1099/ijs.0.63527-0 Medline.[CrossRef]
    [Google Scholar]
  4. Bowman J.P. , McCammon S.A. , Brown J.L. , Nichols P.D. , McMeekin T.A. . ( 1997;). Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47: 670–677 doi:10.1099/00207713-47-3-670 Medline.[CrossRef]
    [Google Scholar]
  5. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 doi:10.1111/j.1432-1033.1970.tb00830.x Medline.[CrossRef]
    [Google Scholar]
  6. Farmer J.J. III , Janda J.M. , Brenner F.W. , Cameron D.N. , Birkhead K.M. , Genus I. . ( 2005;). Genus I. Vibrio Pacini 1854, 411AL. . In Bergey's Manual of Systematic Bacteriology, pp. 494–546. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . 2 , 2nd edn.., New York: Springer;.
    [Google Scholar]
  7. Feller G. , Gerday C. . ( 2003;). Psychrophilic enzymes: hot topics in cold adaptation. Nat Rev Microbiol 1: 200–208 doi:10.1038/nrmicro773 Medline.[CrossRef]
    [Google Scholar]
  8. Huß V.A.R. , Festl H. , Schleifer K.-H. . ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 doi:10.1016/S0723-2020(83)80048-4 Medline.[CrossRef]
    [Google Scholar]
  9. Kim O.S. , Cho Y.J. , Lee K. , Yoon S.H. , Kim M. , Na H. , Park S.C. , Jeon Y.S. , Lee J.H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 doi:10.1099/ijs.0.038075-0 Medline.[CrossRef]
    [Google Scholar]
  10. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar H. , Buchner A. , Lai T. , Steppi S. , other authors . ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32: 1363–1371 doi:10.1093/nar/gkh293 Medline.[CrossRef]
    [Google Scholar]
  11. Macián M.C. , Pujalte M.J. , Márquez M.C. , Ludwig W. , Ventosa A. , Garay E. , Schleifer K.H. . ( 2002;). Gelidibacter mesophilus sp. nov., a novel marine bacterium in the family Flavobacteriaceae . Int J Syst Evol Microbiol 52: 1325–1329 doi:10.1099/ijs.0.02009-0 Medline.[CrossRef]
    [Google Scholar]
  12. Margesin R. . ( 2009;). Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. Extremophiles 13: 257–262 doi:10.1007/s00792-008-0213-3 Medline.[CrossRef]
    [Google Scholar]
  13. Margesin R. , Zhang D.-C. . ( 2013;). Humitalea rosea gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium of the family Acetobacteraceae isolated from soil. Int J Syst Evol Microbiol 63: 1411–1416 doi:10.1099/ijs.0.043018-0 Medline.[CrossRef]
    [Google Scholar]
  14. Margesin R. , Gander S. , Zacke G. , Gounot A.M. , Schinner F. . ( 2003;). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7: 451–458 doi:10.1007/s00792-003-0347-2 Medline.[CrossRef]
    [Google Scholar]
  15. Margesin R. , Schinner F. , Marx J. C. , Gerday C. . ), ( 2008;). Psychrophiles: from Biodiversity to Biotechnology. ., Berlin: Springer;.
  16. Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 doi:10.1016/S0022-2836(62)80066-7 Medline.[CrossRef]
    [Google Scholar]
  17. Morita R.Y. . ( 1975;). Psychrophilic bacteria. Bacteriol Rev 39: 144–167 Medline.
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 Medline.
    [Google Scholar]
  19. Sambrook J. , Frisch E.F. , Maniatis T. . ( 1989;). Molecular Cloning: a Laboratory Manual , 2nd edn.., Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  20. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 MIDI: Newark, DE;.
  21. Süßmuth R. , Eberspächer J. , Haag R. , Springer W. . ( 1987;). Biochemisch-mikrobiologisches Praktikum Stuttgart: Georg Thieme;.
    [Google Scholar]
  22. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 doi:10.1093/molbev/msr121 Medline.[CrossRef]
    [Google Scholar]
  23. Tindall B.J. . ( 1990a;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66: 199–202 doi:10.1111/j.1574-6968.1990.tb03996.x.[CrossRef]
    [Google Scholar]
  24. Tindall B.J. . ( 1990b;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13: 128–130 doi:10.1016/S0723-2020(11)80158-X.[CrossRef]
    [Google Scholar]
  25. Wayne L.G. , Brenner D.J. , Colwell R.R. , Grimont P.A.D. , Kandler O. , Krichevsky M.I. , Moore L.H. , Moore W.E.C. , Murray R.G.E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 doi:10.1099/00207713-37-4-463.[CrossRef]
    [Google Scholar]
  26. Zhang D.-C. , Margesin R. . ( 2014;). Martelella radicis sp. nov. and Martelella mangrovi sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 64: 3104–3108 doi:10.1099/ijs.0.066373-0 Medline.[CrossRef]
    [Google Scholar]
  27. Zhang D.-C. , Wang H.-X. , Liu H.-C. , Dong X.-Z. , Zhou P.-J. . ( 2006;). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56: 2921–2925 doi:10.1099/ijs.0.64564-0 Medline.[CrossRef]
    [Google Scholar]
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