1887

Abstract

An orange-pigmented, Gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13 was isolated from the brood pouch of a diseased seahorse male of the species from the animal facility of the University of Giessen, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13 into the monophyletic cluster of the genus within the family . However, the strain shared only 92.2–93.8 % sequence similarity to type strains of species of the genus , with highest sequence similarity to the type strain of Cellular fatty acid analysis showed a -typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C (40.9 %) followed by iso-C 3-OH (14.8 %). In the polyamine pattern, -homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The quinone system contained exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate group, an amino group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13 was 30 mol%. Based on genotypic, chemotaxonomic and physiological characterizations we propose a novel species of the genus , sp. nov., with strain 96_Hippo_TS_3/13 ( = CIP 110839 =  LMG 28572 = CCM 8557) as the type strain. An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000245
2015-07-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2241.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000245&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. ( 1996;). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47 3952 [View Article].
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  3. Brosius J., Dull T.J., Sleeter D.D., Noller H.F. ( 1981;). Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli . J Mol Biol 148 107127 [View Article] [PubMed].
    [Google Scholar]
  4. Busse H.-J., Auling G. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11 18 [View Article].
    [Google Scholar]
  5. Busse H.-J., Bunka S., Hensel A., Lubitz W. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47 698708 [View Article].
    [Google Scholar]
  6. Choi A., Baek K., Lee H., Cho J.C. ( 2015;). Mesonia aquimarina sp. nov., a marine bacterium isolated from coastal seawater. Int J Syst Evol Microbiol 65 135140 [View Article] [PubMed].
    [Google Scholar]
  7. Coloqhoun J.A. ( 1997;). Discovery of Deep-Sea Actinomycetes. ., PhD Dissertation. Research School of Biosciences. University of Kent, Canterbury .
    [Google Scholar]
  8. Felsenstein J. ( 1985;). Confidence limits on phylogenies :  an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  9. Felsenstein J. ( 2005;). phylip – phylogeny inference package (version 3.6). Distributed by the author. Department of Genome Sciences; University of Washington, Seattle, USA:.
    [Google Scholar]
  10. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors), ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC: :  American Society for Microbiology;.
    [Google Scholar]
  11. Glaeser S.P., Falsen E., Martin K., Kämpfer P. ( 2013;). Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen. Int J Syst Evol Microbiol 63 36233627 [View Article] [PubMed].
    [Google Scholar]
  12. Gonzalez J.M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773 [View Article] [PubMed].
    [Google Scholar]
  13. Hamana K., Nakagawa Y. ( 2001;). Polyamine distribution profiles in the eighteen genera phylogenetically located within the Flavobacterium-Flexibacter-Cytophaga complex. Microbios 106 717 [PubMed].
    [Google Scholar]
  14. Jukes T.H., Cantor C.R. ( 1969;). Evolution of the protein molecules. In. Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. New York: :  [View Article] Academic Press;.
    [Google Scholar]
  15. Kämpfer P., Kroppenstedt R.M. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42 9891005 [View Article].
    [Google Scholar]
  16. Kang H.S., Lee S.D. ( 2010;). Mesonia phycicola sp. nov., isolated from seaweed, and emended description of the genus Mesonia. Int J Syst Evol Microbiol 60 591594 [View Article] [PubMed].
    [Google Scholar]
  17. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H. other authors ( 2012;). Introducing EzTaxon-e :  a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  18. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: :  Wiley;.
    [Google Scholar]
  19. Lee S.Y., Lee M.H., Yoon J.H. ( 2012;). Mesonia ostreae sp. nov., isolated from seawater of an oyster farm, and emended description of the genus Mesonia. Int J Syst Evol Microbiol 62 18041808 [View Article] [PubMed].
    [Google Scholar]
  20. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004;). arb :  a software environment for sequence data. Nucleic Acids Res 32 13631371 [View Article] [PubMed].
    [Google Scholar]
  21. Nedashkovskaya O.L., Kim S.B. ( 2011;). Genus XXXIV. Mesonia Nedashkovskaya, Kim, Han, Lysenko, Rohde, Zhukova, Falsen, Frolova, Mikhailov, and Bae 2003a, 1970VP emend. Nedashkovskaya, Kim, Zhukova, Kwak, Mikhailov and Bae 2006e, 2435. . In Bergey's Manual of Systematic Bacteriology vol. 4, 2nd edn., pp. 239240. Edited by Krieg N. R., Parte A., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N. L., Brown D. R. , 2nd edn.., New York: :  Springer;.
    [Google Scholar]
  22. Nedashkovskaya O.I., Kim S.B., Han S.K., Lysenko A.M., Rohde M., Zhukova N.V., Falsen E., Frolova G.M., Mikhailov V.V., Bae K.S. ( 2003;). Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53 19671971 [View Article] [PubMed].
    [Google Scholar]
  23. Nedashkovskaya O.I., Kim S.B., Zhukova N.V., Kwak J., Mikhailov V.V., Bae K.S. ( 2006;). Mesonia mobilis sp. nov., isolated from seawater, and emended description of the genus Mesonia. Int J Syst Evol Microbiol 56 24332436 [View Article] [PubMed].
    [Google Scholar]
  24. Pitcher D.G., Saunders N.A., Owen R.J. ( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8 151156 [View Article].
    [Google Scholar]
  25. Pruesse E., Quast C., Knittel K., Fuchs B.M., Ludwig W., Peplies J., Glöckner F.O. ( 2007;). silva :  a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . Nucleic Acids Res 35 71887196 [View Article] [PubMed].
    [Google Scholar]
  26. Pruesse E., Peplies J., Glöckner F.O. ( 2012;). sina :  accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28 18231829 [View Article] [PubMed].
    [Google Scholar]
  27. Reichenbach H. ( 1992;). Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List No. 41. Int J Syst Bacteriol 42 327328 [View Article].
    [Google Scholar]
  28. Schumann P. ( 2011;). Peptidoglycan Structure. Methods Microbiol 38 101129 [View Article].
    [Google Scholar]
  29. Seiler H., Bleicher A., Busse H.-J., Hüfner J., Scherer S. ( 2012;). Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese. Int J Syst Evol Microbiol 62 18501856 [View Article] [PubMed].
    [Google Scholar]
  30. Stamatakis A. ( 2006;). RAxML-VI-HPC :  maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 26882690 [View Article] [PubMed].
    [Google Scholar]
  31. Stolz A., Busse H.-J., Kämpfer P. ( 2007;). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57 572576 [View Article] [PubMed].
    [Google Scholar]
  32. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5 :  molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  33. Tindall B.J. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128130 [View Article].
    [Google Scholar]
  34. Tindall B.J. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66 199202 [View Article].
    [Google Scholar]
  35. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K.H., Ludwig W., Glöckner F.O., Rosselló-Móra R. ( 2008;). The All-Species Living Tree project :  a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31 241250 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000245
Loading
/content/journal/ijsem/10.1099/ijs.0.000245
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error