1887

Abstract

A halotolerant, Gram-negative, rod-shaped and light-red-pigmented bacterium, designated LP51, was isolated from pond sediment near a hexachlorocyclohexane dumpsite located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LP51 formed a distinct phyletic clade along with the members of the genus The 16S rRNA gene sequence similarity to members of the genus ranged from 94.2 to 99.4  %. The cells were motile, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C (17.8  %), iso-C 3-OH (8.8  %), iso-C 3-OH (5.7  %), summed feature 3 (Cω7 and/or Cω6; 6.5  %) and summed feature 4 (iso-C I and/or anteiso-C B; 30.7  %). The polar lipid profile of strain LP51 showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids, unknown polar lipids and unknown glycolipids. DNA–DNA relatedness of strain LP51 with respect to the most closely related type strain, X14-1, was 47.2  %. On the basis of this information, it is proposed that the isolate be assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LP51 ( = CCM 8436 = MCC 2070).

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2015-07-01
2024-12-05
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References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. ( 1990;). Basic local alignment search tool. J Mol Biol 215 403410 [View Article] [PubMed].
    [Google Scholar]
  2. Arden Jones M.P., McCarthy A.J., Cross T. ( 1979;). Taxonomic and serological studies on Micropolyspora faeni Micropolyspora strains from soil bearing the specific epithet rectivirgula . J Gen Microbiol 115 343354 [View Article] [PubMed].
    [Google Scholar]
  3. Bernardet J.F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  4. Bligh E.G., Dyer W.J. ( 1959;). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37 911917 [View Article] [PubMed].
    [Google Scholar]
  5. Bowman J.P. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50 18611868 [PubMed].
    [Google Scholar]
  6. Bowman J.P., Nichols C.M., Gibson J.A.E. ( 2003;). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53 13431355 [View Article] [PubMed].
    [Google Scholar]
  7. Busse J., Auling G. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11 18 [View Article].
    [Google Scholar]
  8. Cao H., Nie Y., Zeng X.C., Xu L., He Z., Luo X., Wu R. ( 2014;). Pontibacter yuliensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 64 968972 [View Article] [PubMed].
    [Google Scholar]
  9. Christensen W.B. ( 1946;). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella Shigella types. J Bacteriol 52 461466 [PubMed].
    [Google Scholar]
  10. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed].
    [Google Scholar]
  11. Cowan S.T., Steel K.J. ( 1965). Manual for the Identification of Medical Bacteria London: Cambridge University Press;.
    [Google Scholar]
  12. Dai J., Xu M., Peng F., Jiang F., Chen X., Wang Z., Fang C. ( 2014;). Pontibacter soli sp. nov., isolated from the soil of a Populus rhizosphere in Xinjiang, China. Antonie van Leeuwenhoek 105 6572 [View Article] [PubMed].
    [Google Scholar]
  13. Dastager S.G., Raziuddin Q.S., Deepa C.K., Li W.J., Pandey A. ( 2010;). Pontibacter niistensis sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 60 28672870 [View Article] [PubMed].
    [Google Scholar]
  14. Dwivedi V., Niharika N., Lal R. ( 2013;). Pontibacter lucknowensis sp. nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 63 309313 [View Article] [PubMed].
    [Google Scholar]
  15. Farmer J.J. III ( 1999;). Enterobacteriaceae: introduction and identification. . In Manual of Clinical Microbiology, pp. 448452. Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. ., , 7th edn.., Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  16. Gonzalez J.M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773 [View Article] [PubMed].
    [Google Scholar]
  17. Gordon R.E., Barnett D.A., Handerhan J.E., Pang C.H.-N. ( 1974;). Nocardia coeliaca Nocardia autotrophica and the nocardin strain. Int J Syst Bacteriol 24 5463 [View Article].
    [Google Scholar]
  18. Joung Y., Kim H., Ahn T.S., Jon K. ( 2011;). Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea. J Microbiol 49 290293 [View Article] [PubMed].
    [Google Scholar]
  19. Joung Y., Kim H., Lee B.-I.I., Kang H., Jang T.Y., Kwon O.S., Joh K. ( 2013;). Pontibacter jeungdoensis sp. nov., isolated from a solar saltern in Korea. J Microbiol 51 531535 [View Article] [PubMed].
    [Google Scholar]
  20. Jukes T., Cantor C.R. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism. Edited by Munro H. N. 3 New York: [View Article] Academic Press;.
    [Google Scholar]
  21. Kang J.Y., Joung Y., Chun J., Kim H., Joh K., Jahng K.Y. ( 2013;). Pontibacter saemangeumensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63 565569 [View Article] [PubMed].
    [Google Scholar]
  22. Kim O.S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  23. Kumar M., Verma M., Lal R. ( 2008;). Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. Int J Syst Evol Microbiol 58 861865 [View Article] [PubMed].
    [Google Scholar]
  24. Kumari R., Subudhi S., Suar M., Dhingra G., Raina V., Dogra C., Lal S., van der Meer J.R., Holliger C., Lal R. ( 2002;). Cloning and characterization of lin genes responsible for the degradation of hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90. Appl Environ Microbiol 68 60216028 [View Article] [PubMed].
    [Google Scholar]
  25. Kuykendall L.D., Roy M.A., O'Neill J.J., Devine T.E. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38 358361 [View Article].
    [Google Scholar]
  26. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  27. Maidak B.L., Cole J.R., Lilburn T.G., Parker C.T. Jr, Saxman P.R., Farris R.J., Garrity G.M., Olsen G.J., Schmidt T.M., Tiedje J.M. ( 2001;). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29 173174 [View Article] [PubMed].
    [Google Scholar]
  28. McCarthy A.J., Cross T. ( 1984;). A taxonomic study of Thermomonospora and other monosporic actinomycetes. J Gen Microbiol 130 525.
    [Google Scholar]
  29. Miller L.T. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16 584586 [PubMed].
    [Google Scholar]
  30. Nedashkovskaya O.I., Kim S.B., Suzuki M., Shevchenko L.S., Lee M.S., Lee K.H., Park M.S., Frolova G.M., Oh H.W., other authors. ( 2005;). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. Int J Syst Evol Microbiol 55 25832588, 2588 [View Article] [PubMed].
    [Google Scholar]
  31. Prakash O., Kumari K., Lal R. ( 2007;). Pseudomonas delhiensis sp. nov., isolated from fly ash dumping site of thermal power plant, Delhi, India. Int J Syst Evol Microbiol 57 527531 [View Article] [PubMed].
    [Google Scholar]
  32. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  33. Singh A.K., Garg N., Sangwan N., Negi V., Kumar R., Vikram S., Lal R. ( 2013;). Pontibacter ramchanderi sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment. Int J Syst Evol Microbiol 63 28292834 [View Article] [PubMed].
    [Google Scholar]
  34. Singh A.K., Garg N., Lata P., Kumar R., Negi V., Vikram S., Lal R. ( 2014;). Pontibacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil. Int J Syst Evol Microbiol 64 254259 [View Article] [PubMed].
    [Google Scholar]
  35. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  36. Srinivasan S., Lee J.J., Lee S.S., Kim M.K. ( 2014;). Pontibacter humi sp. nov., isolated from mountain soil. Curr Microbiol 69 263269 [View Article] [PubMed].
    [Google Scholar]
  37. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch.V. ( 2013;). Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63 45244532 [View Article] [PubMed].
    [Google Scholar]
  38. Subhash Y., Sasikala Ch., Ramana Ch.V. ( 2014;). Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov., isolated from the desert. Int J Syst Evol Microbiol 64 10061011 [View Article] [PubMed].
    [Google Scholar]
  39. Suresh K., Mayilraj S., Chakrabarti T. ( 2006;). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Flexibacteraceae’. Int J Syst Evol Microbiol 56 17031707 [View Article] [PubMed].
    [Google Scholar]
  40. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  41. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  42. Tourova T.P., Antonov A.S. ( 1988;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19 333355 [View Article].
    [Google Scholar]
  43. Vanbroekhoven K., Ryngaert A., Bastiaens L., Wattiau P., Vancanneyt M., Swings J., De Mot R., Springael D. ( 2004;). Streptomycin as a selective agent to facilitate recovery and isolation of introduced and indigenous Sphingomonas from environmental samples. Environ Microbiol 6 11231136 [View Article] [PubMed].
    [Google Scholar]
  44. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. ( 1996;). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60 407438 [PubMed].
    [Google Scholar]
  45. Wang Y., Zhang K., Cai F., Zhang L., Tang Y., Dai J., Fang C. ( 2010;). Pontibacter xinjiangensis sp. nov., in the phylum ‘Bacteroidetes’, and reclassification of [Effluviibacter roseus as Pontibacter roseus comb. nov. Int J Syst Evol Microbiol 60 99103 [View Article] [PubMed].
    [Google Scholar]
  46. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  47. Xu M., Wang Y., Dai J., Jiang F., Rahman E., Peng F., Fang C. ( 2012;). Pontibacter populi sp. nov., isolated from the soil of a Euphrates poplar (Populus euphratica) forest. Int J Syst Evol Microbiol 62 665670 [View Article] [PubMed].
    [Google Scholar]
  48. Xu L., Zeng X.C., Nie Y., Luo X., Zhou E., Zhou L., Pan Y., Li W. ( 2014;). Pontibacter diazotrophicus sp. nov., a novel nitrogen-fixing bacterium of the family Cytophagaceae . PLoS One 9 e92294 [View Article] [PubMed].
    [Google Scholar]
  49. Zhang L., Zhang Q., Luo X., Tang Y., Dai J., Li Y., Wang Y., Chen G., Fang C. ( 2008;). Pontibacter korlensis sp. nov., isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 58 12101214 [View Article] [PubMed].
    [Google Scholar]
  50. Zhang L., Zhu L., Wei L., Li C., Wang Y., Shen X. ( 2013;). Pontibacter toksunensis sp. nov., isolated from soil, and emended descriptions of Pontibacter roseus Pontibacter akesuensis . Int J Syst Evol Microbiol 63 44624468 [View Article] [PubMed].
    [Google Scholar]
  51. Zhou Y., Wang X., Liu H., Zhang K.Y., Zhang Y.Q., Lai R., Li W.J. ( 2007;). Pontibacter akesuensis sp. nov., isolated from a desert soil in China. Int J Syst Evol Microbiol 57 321325 [View Article] [PubMed].
    [Google Scholar]
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