1887

International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_7

sp. nov., isolated from decomposing algal scum Free

Abstract

Bacterial strain THMBR28 was isolated from decomposing algal scum that was collected during an algal bloom in Taihu lake, China. Cells of strain THMBR28 were Gram-staining-positive, facultatively anaerobic and rod-shaped. Growth was observed at 20–45 °C (optimum, 30 °C), at pH 5.0–9.5 (optimum, pH 6.5–7.5), and in the presence of 0–1.0  % (w/v) NaCl (optimum, 0.5  %). Strain THMBR28 contained MK-7 as the major menaquinone and iso-C as the major cellular fatty acid. The polar lipid profile contained phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and six unidentified polar lipids. The diamino acid found in the cell-wall peptidoglycan was diaminopimelic acid. The DNA G+C content was 57.6 mol% ( ). Phylogenetic analysis of 16S rRNA gene sequences showed that strain THMBR28 belonged to the genus , most closely related to DSM 18389 (95.0  %) and Eur1 9.5 (93.4  %). Based on phylogenetic and phenotypic characterization, it is concluded that strain THMBR28 represents a novel species of the genus , for which the name sp. nov. is proposed, with THMBR28 ( = CGMCC 1.10949 = NBRC 108765) as the type strain.

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000240
2015-07-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2194.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000240&mimeType=html&fmt=ahah

References

  1. Baek S.H., Cui Y., Kim S.C., Cui C.H., Yin C., Lee S.T., Im W.T. ( 2011;). Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 61 1715–1719 [View Article] [PubMed].
     [Google Scholar]
  2. Barrow G.I., Feltham R.K.A. ( 1993). Cowan and Steel's Manual for Identification of Medical Bacteria , 3rd edn.. Cambridge: Cambridge University Press; doi:10.1017/CBO9780511527104 . [CrossRef]
    [Google Scholar]
  3. Collins M.D. ( 1985;). Isoprenoid quinone analysis in bacterial classificaion and identification. . In Chemical Methods in Bacterial Sytematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
     [Google Scholar]
  4. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376. [CrossRef]
    [Google Scholar]
  5. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  6. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  7. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  8. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161–207. [CrossRef]
    [Google Scholar]
  9. Lányí B. ( 1987;). Classical and rapid identification methods for medi-cally important bacteria. Methods Microbiol 19 1–67. [CrossRef]
    [Google Scholar]
  10. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109–118 [View Article] [PubMed].
     [Google Scholar]
  11. Minnikin D.E., Odonnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  12. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  13. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. ., MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  14. Schaeffer A.B., Fulton M.D. ( 1933;). A simplified method of staining endospores. Science 77 194 [View Article] [PubMed].
     [Google Scholar]
  15. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  16. Steven B., Chen M.Q., Greer C.W., Whyte L.G., Niederberger T.D. ( 2008;). Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost. Int J Syst Evol Microbiol 58 1497–1501 [View Article] [PubMed].
     [Google Scholar]
  17. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 2725–2729 [View Article] [PubMed].
     [Google Scholar]
  18. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  19. Wang Q., Xie N., Qin Y., Shen N., Zhu J., Mi H., Huang R. ( 2013;). Tumebacillus flagellatus sp. nov., an α-amylase/pullulanase-producing bacterium isolated from cassava wastewater. Int J Syst Evol Microbiol 63 3138–3142 [View Article] [PubMed].
     [Google Scholar]
  20. Wu Y.F., Wu Q.L., Liu S.J. ( 2012;). Chryseobacterium taihuense sp. nov., isolated from aa eutrophic lake, and emended descriptions of the genus Chryseobacterium Chryseobacterium taiwanense Chryseobacterium jejuense Chryseobacterium indoltheticum . Int J Syst Evol Microbiol 63 913–919 [View Article] [PubMed].
     [Google Scholar]
  21. Zhang D., Yang H., Zhang W., Huang Z., Liu S.J. ( 2003;). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53 1111–1114 [View Article] [PubMed].
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000240
Loading
Tumebacillus algifaecis sp. nov., isolated from decomposing algal scum
Int J Syst Evol Microbiol 65, 2194 (2015); https://doi.org/10.1099/ijs.0.000240
/content/journal/ijsem/10.1099/ijs.0.000240
/content/journal/ijsem/10.1099/ijs.0.000240
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error