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Abstract

Bacterial strain THMBR28 was isolated from decomposing algal scum that was collected during an algal bloom in Taihu lake, China. Cells of strain THMBR28 were Gram-staining-positive, facultatively anaerobic and rod-shaped. Growth was observed at 20–45 °C (optimum, 30 °C), at pH 5.0–9.5 (optimum, pH 6.5–7.5), and in the presence of 0–1.0  % (w/v) NaCl (optimum, 0.5  %). Strain THMBR28 contained MK-7 as the major menaquinone and iso-C as the major cellular fatty acid. The polar lipid profile contained phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and six unidentified polar lipids. The diamino acid found in the cell-wall peptidoglycan was diaminopimelic acid. The DNA G+C content was 57.6 mol% ( ). Phylogenetic analysis of 16S rRNA gene sequences showed that strain THMBR28 belonged to the genus , most closely related to DSM 18389 (95.0  %) and Eur1 9.5 (93.4  %). Based on phylogenetic and phenotypic characterization, it is concluded that strain THMBR28 represents a novel species of the genus , for which the name sp. nov. is proposed, with THMBR28 ( = CGMCC 1.10949 = NBRC 108765) as the type strain.

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2015-07-01
2020-09-30
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References

  1. Baek S.H., Cui Y., Kim S.C., Cui C.H., Yin C., Lee S.T., Im W.T. ( 2011;). Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 61 17151719 [CrossRef] [PubMed].
    [Google Scholar]
  2. Barrow G.I., Feltham R.K.A. ( 1993). Cowan and Steel's Manual for Identification of Medical Bacteria , 3rd edn.. Cambridge: Cambridge University Press; doi:10.1017/CBO9780511527104 . [CrossRef]
    [Google Scholar]
  3. Collins M.D. ( 1985;). Isoprenoid quinone analysis in bacterial classificaion and identification. . In Chemical Methods in Bacterial Sytematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  4. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376. [CrossRef]
    [Google Scholar]
  5. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  6. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [CrossRef].
    [Google Scholar]
  7. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  8. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161207. [CrossRef]
    [Google Scholar]
  9. Lányí B. ( 1987;). Classical and rapid identification methods for medi-cally important bacteria. Methods Microbiol 19 167. [CrossRef]
    [Google Scholar]
  10. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [CrossRef] [PubMed].
    [Google Scholar]
  11. Minnikin D.E., Odonnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [CrossRef].
    [Google Scholar]
  12. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  13. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. ., MIDI Technical Note 101 Newark, DE: MIDI Inc;.
  14. Schaeffer A.B., Fulton M.D. ( 1933;). A simplified method of staining endospores. Science 77 194 [CrossRef] [PubMed].
    [Google Scholar]
  15. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  16. Steven B., Chen M.Q., Greer C.W., Whyte L.G., Niederberger T.D. ( 2008;). Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost. Int J Syst Evol Microbiol 58 14971501 [CrossRef] [PubMed].
    [Google Scholar]
  17. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [CrossRef] [PubMed].
    [Google Scholar]
  18. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [CrossRef] [PubMed].
    [Google Scholar]
  19. Wang Q., Xie N., Qin Y., Shen N., Zhu J., Mi H., Huang R. ( 2013;). Tumebacillus flagellatus sp. nov., an α-amylase/pullulanase-producing bacterium isolated from cassava wastewater. Int J Syst Evol Microbiol 63 31383142 [CrossRef] [PubMed].
    [Google Scholar]
  20. Wu Y.F., Wu Q.L., Liu S.J. ( 2012;). Chryseobacterium taihuense sp. nov., isolated from aa eutrophic lake, and emended descriptions of the genus Chryseobacterium Chryseobacterium taiwanense Chryseobacterium jejuense Chryseobacterium indoltheticum . Int J Syst Evol Microbiol 63 913919 [CrossRef] [PubMed].
    [Google Scholar]
  21. Zhang D., Yang H., Zhang W., Huang Z., Liu S.J. ( 2003;). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53 11111114 [CrossRef] [PubMed].
    [Google Scholar]
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