Two isolates from water, D16Q19 and D16R27, were shown to be highly similar in their 16S rRNA, 16S–23S internal transcribed spacer (ITS), hsp65 and rpoB gene sequences to ‘Mycobacterium franklinii’ DSM 45524, described in 2011 but with the name not validly published. They are all nonpigmented rapid growers and are related phenotypically and genetically to the Mycobacterium chelonae–Mycobacterium abscessus group. Extensive characterization by phenotypic analysis, biochemical tests, drug susceptibility testing, PCR restriction enzyme analysis of the hsp65 gene and ITS, DNA sequencing of housekeeping genes and DNA–DNA hybridization demonstrated that ‘M. franklinii’ DSM 45524, D16Q19 and D16R27 belong to a single species that is separated from other members of the M. chelonae–M. abscessus group. On the basis of these results we propose the formal recognition of Mycobacterium franklinii sp. nov. Strain DSM 45524T ( = ATCC BAA-2149T) is the type strain.
AdékambiT.,
Reynaud-GaubertM.,
GreubG.,
GevaudanM.J.,
La ScolaB.,
RaoultD.,
DrancourtM.
( 2004;). Amoebal coculture of “Mycobacterium massiliense” sp. nov. from the sputum of a patient with hemoptoic pneumonia. J Clin Microbiol425493–5501
doi:10.1128/JCM.42.12.5493-5501.2004 Medline . [CrossRef]
AdékambiT.,
Reynaud-GaubertM.,
GreubG.,
GevaudanM.J.,
La ScolaB.,
RaoultD.,
DrancourtM.
( 2006b;). Mycobacterium massiliense sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 111. Int J Syst Evol Microbiol562025–2027
doi:10.1099/ijs.0.64643-0 . [CrossRef]
Brown-ElliottB.A.,
WallaceR.J.Jr
( 2012;). Nontuberculous mycobacteria. . In Hospital Epidemiology and Infection Control, pp. 593–608. Edited by
MayhallC. G.
, 4th edn.
Philadelphia, PA:
Lippincott Williams & Wilkins;.
DevulderG.,
Pérouse de MontclosM.,
FlandroisJ.P.
( 2005;). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int J Syst Evol Microbiol55293–302
doi:10.1099/ijs.0.63222-0 Medline . [CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.
( 1989;). Flourometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39224–229
doi:10.1099/00207713-39-3-224 . [CrossRef]
GomilaM.,
RamirezA.,
LalucatJ.
( 2007;). Diversity of environmental Mycobacterium isolates from hemodialysis water as shown by a multigene sequencing approach. Appl Environ Microbiol733787–3797
doi:10.1128/AEM.02934-06 Medline . [CrossRef]
LeãoS.C.,
TortoliE.,
Viana-NieroC.,
UekiS.Y.,
LimaK.V.,
LopesM.L.,
YuberoJ.,
MenendezM.C.,
GarciaM.J.
( 2009;). Characterization of mycobacteria from a major Brazilian outbreak suggests that revision of the taxonomic status of members of the Mycobacterium chelonae-M. abscessus group is needed. J Clin Microbiol472691–2698
doi:10.1128/JCM.00808-09 Medline . [CrossRef]
MarmurJ.
( 1961;). A procedure for isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol3208–218
doi:10.1016/S0022-2836(61)80047-8 . [CrossRef]
MesbahM.,
WhitmanW.B.
( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A479297–306
doi:10.1016/S0021-9673(01)83344-6 Medline . [CrossRef]
PitcherD.G.,
SaundersN.A.,
OwenR.J.
( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol8151–156
doi:10.1111/j.1472-765X.1989.tb00262.x . [CrossRef]
TelentiA.,
MarchesiF.,
BalzM.,
BallyF.,
BöttgerE.C.,
BodmerT.
( 1993;). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol31175–178
Medline .
WhippsC.M.,
ButlerW.R.,
PourahmadF.,
WatralV.G.,
KentM.L.
( 2007;). ). Molecular systematics support the revival of Mycobacterium salmoniphilum (ex Ross 1960) sp. nov., nom. rev., a species closely related to Mycobacterium chelonae
. Int J Syst Evol Microbiol572525–2531
doi:10.1099/ijs.0.64841-0 Medline . [CrossRef]
WilsonR.W.,
SteingrubeV.A.,
BöttgerE.C.,
SpringerB.,
Brown-ElliottB.A.,
VincentV.,
JostK.C.Jr,
ZhangY.,
GarciaM.J.,
other authors.
( 2001;). Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy. Int J Syst Evol Microbiol511751–1764
doi:10.1099/00207713-51-5-1751 Medline . [CrossRef]