1887

Abstract

A Gram-staining negative, rod-shaped bacterium, designated strain YIM 78110, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China. The taxonomic status of strain YIM 78110 was confirmed by a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain YIM 78110 belongs to the genus , displaying 96.8 % and 94.7 % sequence similarity with the two most closely related type strains, RQ-33 and LW9, respectively. The low value of DNA–DNA hybridization (52.3 ± 2.3 %) between strain YIM 78110 and its closest neighbour, RQ-33, indicated that this new isolate represented a different genomic species in the genus . The temperature for growth ranged from 30 to 50 °C. The pH for growth ranged from pH 4.0 to 10.0, with NaCl tolerance of 0.5–6.0 % (w/v). The predominant menaquinone of strain YIM 78110 was MK-7 and the major polar lipid was phosphatidylethanolamine. The major fatty acids were iso-C and C. The DNA G+C content was 47.1 mol%. On the basis of physiological, biochemical and phylogenetic analyses, it is proposed that strain YIM 78110 represents a novel species of the genus , for which the name Cecembia sp. nov. is proposed. The type strain is YIM 78110 ( = CCTCC AB2013287 = DSM 28057).

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2015-07-01
2020-09-29
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